Posted by admin on Jun 1, 2014 in |
In the present endeavour, bioprospecting potentials (antimicrobial activity, antibiotic susceptibility pattern, enzyme activity and dye degradation ability) of endophytic bacteria isolated from different plants were explored. Total of thirty bacterial endophytes were isolated from the leaves of selected plants by using sterilization treatment followed by serial dilution agar plate technique. All the isolates were evaluated for the antimicrobial activity against 7 pathogenic strains including 2 Gram positive bacteria (Staphylococcus epidermidis and Bacillus amyloliquifaciens)and 2 Gram-negative bacteria (Escherichia coli and Salmonella enterica ser. typhi)and 3 fungi (Aspergillus fumigatus, Aspergillus sp. and Candida albicans) using agar well diffusion method. Of the 30 bacterial isolates, twenty endophytic bacterial isolates exhibited both antifungal and antibacterial activity. It was observed that 33.33% of isolates exhibited urease activity, 66.66% amylase activity, while 50% esterase activity. Malachite green degradation was observed in 16 (53.33%) endophytic bacterial isolates. The antibiotic susceptibility pattern of endophytic bacterial isolates was evaluated. Five Gram negative bacterial isolates were sensitive to Amikacin, Levofloxacin, Cephotaxime, four isolates sensitive to Aztreonam and three isolates...
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Posted by admin on Jun 1, 2014 in |
Background & objectives: Liver diseases are one of the major causes of disease burden all over world including our country so it is the need of hour to search for alternative drugs for the treatment of liver diseases. Aloe Vera is one of the most popular plants in the world, and is has been used frequently in history for its medicinal properties. Material &Methods: A total of 110 male and female patients of age group between 15- 65 years, diagnosed clinically and biochemically as the case of acute viral hepatitis and ready to give consent were recruited in the study from SVBP Hospital, LLRM Medical College Meerut (U.P), 250004. Subjects who fulfilled selection criteria were randomized into two groups. Fifty patients belonging to control group received conventional treatment for acute viral hepatitis while 60 patients enrolled in treated group were given conventional treatment for acute viral hepatitis supplemented with Aloe vera juice (Patanjali Ayurved ltd, Haridwar) in dose of 20 ml BD orally. Every patient was followed up for...
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Posted by admin on Jun 1, 2014 in |
The coriander seedextract in 80% methanol is examined for the polyphenol composition and the antimicrobial potential against pathogenic bacteria Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus pumilus. Gallic acid, caffeic acid, ellagic acid, quercetin and kaempferol are principle polyphenolics identified and quantified in the coriander extract by HPLC. The antimicrobial activity was examined by determining cell damage and measuring cell inhibition zone. The growth inhibition zones observed by agar well diffusion method are 11.93 to 17.27 mm in diameter in presence of coriander extract. Minimum inhibitory concentration of coriander was 4.16 mg/ml for the bacteria tested. Increased release of intracellular nucleotides and proteinaceous materials from the bacterial cells in the presence of methanolic coriander extract suggests that the primary mechanism of action of coriander extract is membrane damage, which leads to cell death. The results obtained herein further encourage the use of coriander in antibacterial formulations due to the fact that polyphenol rich coriander extract could effectively kill pathogenic bacteria related to foodborne...
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Posted by admin on Jun 1, 2014 in |
Bio-efficacy of plants and their derivatives have been reemphasized in recent times. The present investigation was carried out to study the antibacterial efficacy of Curcuma caesia (family: Zingiberaceae) commonly known as kali haldi. The root, stem and leaf of Curcuma caesia were extracted successively with polar (aqueous, methanol), dipolar (acetone) and non-polar (chloroform) solvents, and their physical characteristics were explored which revealed the presence of more phytocompounds in case of root followed by stem and leaf. The extracts were assessed for their potential antibacterial activity against gram positive and gram negative bacteria viz., Bacillus cerus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa and Klebsiella pneumoneae. The gram positive bacteria were found to be more sensitive than gram negative bacteria. The inhibition of both gram positive and gram negative bacteria by the extracts indicates the presence of broad spectrum antibacterial potentiality of the plant. The root extracts of the plant were found to be more effective in inhibiting the bacterial growth as compared to stem...
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Posted by admin on Jun 1, 2014 in |
The chloroform extracts of S. asoca bark were studied for the wound healing potential. The wound healing activity of S. asoca bark by excision, incision and dead space wound models in albino Wistar rats and antioxidant profile of this bark extract were also studied as there is an increase in the levels of free radicals during the damage of tissue. The parameters like wound contraction, period of epithelialisation, tensile strength and hydroxyproline content were evaluated in three wound models, namely; excision, incision and dead space wound models. Besides, their influences on oxidative stress in three wound models were also estimated. The results obtained were compared with standard drug nitrofurazone and control in terms of wound contraction, period of epithelialisation, tensile strength and hydroxyproline content. The chloroform extract of S. asoca bark showed significant results in all models when compared to standard and control. A subservient study made on the levels of superoxide-dismutase, catalase, glutathione, vitamin C and lipid peroxidation are recorded and a significant increase in the levels of...
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