Posted by admin on Mar 1, 2014 in |
Microbial fibrinolytic proteases are of considerable interest in view of their specific activity and stability at biological pH. This review describes different isolation methods which enable the screening and selection of promising organisms for industrial production. Further, strain improvement using mutagenesis and/or recombinant DNA technology can be applied to augment the efficiency of the producer strain to a commercial status. The various nutritional and environmental parameters affecting the production of fibrinolytic proteases are delineated. The purification and properties of these fibrinolytic proteases is discussed, and the use of fibrinolytic proteases is...
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Posted by admin on Mar 1, 2014 in |
A simple, precise, accurate and stability indicating RP-HPLC method was developed and validated for the determination of choline fenofibrate in delayed release tablet dosage form. Separation was achieved under optimized chromatographic conditions on an inertsil ODS column (150 x 4.6 mm, 5µ particle size). The mobile phase consisted of potassium dihydrogen phosphate buffer pH 2.5 and acetonitrile in the ratio 32:68, an isocratic elution at a flow rate of 1.5mL/minute at ambient temperature. The detection was carried out at 300nm using Shimadzu UV-visible detector HPLC system. The retention time of fenofibrate was found at 3.70 minutes and the calibration curve was linear in the concentration range of 18-42µg/mL (r2=1). The limit of detection and the limit of quantification were found to be 2.40µg/mL and 7.28µg/mL respectively. The amount of fenofibrate present in dosage form was 100.9%. The proposed method was validated as per the ICH guidelines and during recovery studies the % recovery was found to be 98.37%. The method was found to be simple, linear, specific, rugged and...
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Posted by admin on Mar 1, 2014 in |
Protocol for efficient callogenesis in Hildegardia populifolia has been developed by using leaf, node, internode and petiole explants. Preliminary phytochemical analysis and quantitative estimation of flavonoids, phenols and tannins were made along with the evaluation of antioxidant properties by DPPH• and ABTS•+ assays in in vitro callus cultures of the same species. It is an indigenous endangered multipurpose medium sized tree being used for traditional medical practices in Tamil Nadu and Andra Pradesh. Among the explants, the efficient callogenesis was accelerated within 10-19 days in leaf explant on Murashige and Skoog (MS) medium supplemented with 2, 4-D alone at 2 mg/L. Effective calli were produced from node (BAP and IAA at 2 mg/L and 0.4 mg/L respectively in MS medium), internode (2,4-D at 2 mg/L in MS medium) and petiole (2,4-D at 2 mg/L in MS medium) explants. The phytochemical study revealed that phenolics and tannin content were higher in 60 days old leaf callus cultures. Flavonoid content was more in 60 days old internode derived callus culture. Evaluation...
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Posted by admin on Mar 1, 2014 in |
Neurodegenerative disorders are heterogeneous group of diseases of the nervous system, including the brain, spinal cord and peripheral nerves that have many different etiologies. Due to the prevalence, morbidity, and mortality of the neurodegenerative diseases, they represent significant medical, social, and financial burden on the society. Dalbergia sissoo (family: Fabaceae) is an Asian deciduous rosewood tree and state tree of Punjab called as Shisham used for aphrodisiac, expectorant, anthelmintic, antipyretic, emesis, ulcers, skin disease, memory enhancer etc. The leaf of extract of D. sissoo having more concentration of flavonoids and its neuroprotective effect in animals is unclear. So this study wasto explore the neuroprotective activity of ethanolic leaf extract of Dalbergia sissoo (ELDS) against chemically induced neurotoxicity in rats. This study was designed with 30 albino wistar rats divides into 5 groups. They were treated for 7 days with glutamate salt followed by ELDS. During treatment physico- pharmacological parameters were recorded, 24hr after experiment i.e on 8th day antioxidant profile from brain isolate were estimated and histopathology of brain...
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Posted by admin on Mar 1, 2014 in |
Background: Bacterial resistance to antimicrobial agents is on the rise and this is causing serious complications resulting in increased morbidity and mortality of bacterial infections. There is a need for new antimicrobial molecules in order to fight against Multi-Drug-Resistant Organisms. The Mediterranean area is rich in a variety of medicinal plants and this may represent a potential for new compounds and molecules with enhanced antibacterial activity. Methods: The antimicrobial effects of three traditionally used Lebanese plants were investigated against 24 clinical isolates of Staphylococcus aureus with different phenotypes of resistance. Rosmarinus officinalis, Rheum rhaponticum, and Origanum majorana where extracted with ethanol, then further subfractionated with petroleum ether, dichloromethane and ethyl acetate. The remaining aqueous fraction was also collected, thus a total of five extracts were studied for each plant. The MIC and MBC of these extracts were determined using the micro-dilution technique. Results: Rosmarinus officinalis was the most effective against most of the strains studied including MRSA, QS, QR, MS and MR. The ethyl acetate fraction of Rosmarinus officinalis, Rheum...
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