Posted by admin on Mar 1, 2014 in |
Delonix elata bark was collected, authenticated, dried, coarse-finely powdered and extracted using 70% alcohol and subjected for preliminary phytochemical screening. Total phenolic and flavonoidal contents were determined using UV/ visible spectrophotometer. In addition the silica column chromatographic separation was carried out to separate the phytochemicals of D. elata bark extract. Preliminary phytochemical screening showed positive tests for saponins, flavonoids, tannins and carbohydrates in the alcoholic extract of Delonix elata bark. When the extract was analyzed quantitatively by UV/ visible spectrophotometer, 75 mg/gm of gallic acid equivalent total phenolics and 57.4 mg/gm of rutin equivalent flavonoids were observed.Two phenolic compounds, caffeic acid and apigenin, were isolated and characterized by spectral analysis from the alcoholic extract of the Delonix elata bark. Moreover, HPLC method was employed to analyze the isolated phytoconstituents i.e. caffeic acid and apigenin present in the fraction of alcoholic extract of D. elata bark which showed the similar Rt of separated constituents. The present study reveals the presence of flavonoids in D. elata bark as evidenced by the phytochemical screening, and...
Read More
Posted by admin on Mar 1, 2014 in |
In Catharanthus roseus, Rutin, Quercetin and Kaempferol are the most important phenolic compounds with antioxidant activity. For simultaneous determination of Rutin, Quercetin and Kaempferol in Catharanthus roseus a simple and rapid HPLC method was developed. Phosphate buffer (pH=5.8) and solvent Acetonitrile are used in ratio of 55: 45 as mobile phase. Rutin, Quercetin and Kaempferol were determined by HPLC by using Athena C18 column and UV absorbance were measured at 254 nm. Retention time (Rt) of standards, Rutin, Quercetin and Kaempferol was found to be 2.357, 6.093 and 9.373 respectively, while in Catharanthus roseus the Retention times of Rutin, Quercetin and Kaempferol are 2.403, 6.143 and 8.903 are found to be matching with standards Retention time values. Thus for quantitative estimation of phenolic compounds, this HPLC method was found to be simple and...
Read More
Posted by admin on Mar 1, 2014 in |
The choice of an antibiotic depends solely on the identification of the species by determination of the sensitivity characteristics of the microorganism. Along with the determination of sensitivity pattern, understanding the susceptibility pattern of particular strain isolated from patient is equally important. Variation in patient and microorganism is known to be key factor for predicting the outcome for individual patient and establishing targets for clinical susceptibility. Dosage adjustment in relation to minimum inhibitory concentration (MIC) of drug, taking into account underlying pathogen might affect the therapeutic response and hence improve clinical outcome of patient. Therefore, E coli positive urine cultures of patients who were prescribed Ciprofloxacin were collected and their MIC was determined by agar well diffusion method. The response of patient was obtained by direct interview with them after 3 days of Ciprofloxacin therapy. There is a direct correlation between MIC and therapeutic outcome of antibiotic therapy. The clinical success rate increases when MIC is <1mg/l whereas, patient becomes non respondent as MIC approaches to >4mg/l which can...
Read More
Posted by admin on Mar 1, 2014 in |
Opportunity is envisaged by some astute pharmaceuticals manufacturers to increase product portfolio and widen the spectrum of treatment of pains to cover analgesia, pyrexia and inflammation using one product. In this research work, paracetamol – ibuprofen combination tablet was designed and formulated, developed and profiled to address this anticipation thus satisfying the requirement of one product fits 3 health challenges. As a consequence of adoption of wet granulation method, granules of acceptable quality profiles were developed and processed and subsequently culminated in production of tablets that met all required critical quality attributes as defined in quality target product profile. Characterization of both granules and tablets showed results that reassured that the requirements of fitness for purpose were met and will lead to product quality and patient safety on the long run. Indeed, content uniformity of active ingredients in each tablet was engendered by equally uniform tablet weight to the extent that relative standard deviation was only 1.29% for uniformity of weight, 1.74% for ibuprofen content and 0.28% for paracetamol...
Read More
Posted by admin on Mar 1, 2014 in |
Kidneys are the excretory organs of our body that serves the important functions such as elimination of waste products, regulation of fluid volume and electrolyte content of the extracellular fluid. Picrorrhiza kurroa (Scrophulariaceae) is a small perennial herb growing in the hilly parts of the north-Western Himalayas region in India and Nepal. The objective of the present study was to evaluate and compare diuretic activity of Picrorrhiza kurroa royle ex. Benth with that of the standard. Two doses of the test extract i.e. 300mg.kg and 500mg.kg body weight orally are used to evaluate the diuretic activity. Furosemide (20mg/kg i.p.) was used as the standard drug to compare the test results. The test extract showed significant diuretic activity (p<0.05) which justify its use in...
Read More
