Posted by admin on Mar 1, 2014 in |
The aim of the present study was to prepare solid lipid nanoparticles (SLNs) for the topical delivery. Tristearin was used as solid lipid with soya lecithin by using surfactant, Poloxamer 188 (1%) and Tween 80 (0.5%). Solid lipid nanoparticles (SLN) loaded with Flucanazole were prepared by solvent diffusion- emulsification method. The properties of the SLNs such as particle size, zeta potential (ZP), Polydispersity index (PI) and drug % entrapment efficiency (% EE) were investigated. The morphology of SLNs was observed by transmission electron microscopy (TEM) and Scanning electron microscopy (SEM). The drug release behavior was studied by in vitro method using franz diffusion cell with dialysis membrane. The results show the formulation F2 had smallest particle size of 122±3.42 nm with Zeta potential -24.03±1.84 and Polydispersity index 0.668±3.21. The % Entrapment efficiency of formulation F2 was found to be 76.53±0.24. The average particles sizes of the nanoparticles were found to increase on storage, which may be due to aggregation of particles. This effect was encountered lower in the case...
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Posted by admin on Mar 1, 2014 in |
Gum Arabic (GA) is a water-soluble polysaccharide, obtained from stems of Acacia senegal trees as gummy exudates. It is a beneficial adjunct to the low-protein diet for chronic renal failure patient because it reduces serum urea nitrogen level. It is also have a good protective activity against CCl4 and acetaminophen-induced hepatotoxicity in rats but it failed protect kidney from gentamicin-induced nephrotoxicity. The goal of current study was to investigate the cardioprotective of gum Arabic aqueous extract against Cardiotoxicity induced by doxorubicin in rats. Wistar albino rats were divided into four groups, 5 rats in each group: Group A (as a control), group B (Doxorubicin (DOX)-only treated rats, 15mg/Kg, IP as a single loading dose), group C (GA 10g/Kg body weight, orally for 4weeks prior DOX treatment) and Group D (GA-only treated group). Histopathological examination and serum biomarker enzymes like Creatine Kinase (CK), Lactate Dehydrogenase (LDH), serum aspartate transaminase (AST) and serum alanine transaminase (ALT) levels were monitored at the end of study to evaluate cardiotoxicity. The study showed that...
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Posted by admin on Mar 1, 2014 in |
Purpose: To compare the efficacy and toxicity of chronomodulated concomitant chemotherapy using weekly Cisplatin at 0600 hour and 1800 hour along with radical external beam radiotherapy (EBRT) in the management of locally advanced head and neck carcinoma (LAHNC). Material and method: Previously untreated, histopathologically proven 60 patients of LAHNC (stage III-IVB) were taken for definitive treatment by concurrent chemo-radiation. These patients were randomly assigned (by draw of lots) either of two groups; group I, the 0600 hour cisplatin administration and group II, the 1800 hour cisplatin administration group, each in dose of 30 mg/m2. EBRT was given as 66Gy/ 33Fr/ 6.5 weeks on telecobalt machine. Night shift workers were excluded. Response to treatment and toxicity were investigated. Observations were made at the end of treatment and 6 months of follow up. Results: At the end of treatment, complete tumor response (CRT) in group II were better (40.0% versus 26.7%) and complete node response (CRN) were comparable (34.8% versus 34.6%). Acute skin and mucosal reactions (grade 3) were 3.3% versus...
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Posted by admin on Mar 1, 2014 in |
Cephalexin remain as one of the most versatile class of compounds against microbes and therefore, are useful substructures for further molecular exploration. A novel series of Schiff bases of cephalexin were synthesized. Thus condensation of 5-thia-1-azabicyclo [4.2.0] oct-2-ene-2-carboxylic acid, 7-[(amino phenyl acetyl) amino]-3-methyl-8-oxo, monohydrate with appropriate aldehydes afforded the Schiff bases. The purity of the compounds was determined by TLC. With elemental analysis and spectral data the structure of the synthesized compounds were elucidated. All the synthesized compounds were subjected for the screening of antimicrobial activity against 3 bacteria and two pathogenic fungi. Most of the synthesized compounds were found to possess moderate antibacterial and antifungal...
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Posted by admin on Mar 1, 2014 in |
A fast, sensitive and accurate reverse phase liquid chromatographic method has been developed and validated for the simultaneous determination of Brinzolamide and Timolol maleate from ophthalmic preparation. Chromatographic separation of these two drugs were achieved on Zorbax Eclipse Plus, Agilent Technology (150mm x 4.6mm, 5μm) as stationary phase with a mobile phase comprising of triethylamine phosphate buffer : Acetonitrile : Methanol (70:20:10(v/v) at a flow rate of 1.0ml / min and PDA detection was set at 274 nm. Injection volume was 10 µl. Column oven temperature was maintained at 40°C. The retention time of timolol, and brinzolamide were 4.0 (±0.5) min, and 10.5 (±0.5) min respectively. Theoretical plate for Brinzolamide and Timolol were 5832 and 4876 respectively. The tailing factor was 1.09 and 1.21 for Brinzolamide and Timolol respectively. Resolution between the two compounds was 16.12. The developed method was validated according to the ICH guidelines and the proposed method can be applied for the routine quality control analysis of Brinzolamide and Timolol maleate from combined dosage...
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