Posted by admin on Feb 1, 2014 in |
The present study describes a simple, accurate and precise RP-HPLC Technique for the simultaneous determination of Flupirtine maleate and Paracetamol in pharmaceutical dosage form. The method involves an isocratic elution of drug in a stationary phase of Phenomenex, C18 (150mm × 4.6mm, 5µm) column using a mobile phase composition of methanol and 0.1% (v/v) orthophosphoric acid in the composition ratio of 60:40 v/v with a flow rate of 0.8 mL/min at 270 nm of detection. The injection volume is 20 µL. the method has been validated for specificity, linearity, range, precision, accuracy, limit of detection, limit of quantification, ruggedness and robustness. The retention times for Flupirtine maleate and Paracetmol are about 3.07 and 4.63 minutes respectively. Quantitative linearity was observed over the concentration range of 10.08 to 302.51 µg/mL for Flupirtine maleate and 4.99 to 99.80 for Paracetamol respectively. The regression equations of concentration of Flupirtine maleate and Paracetamol are found to be y = 1774x+4755, y = 39182x + 64154 respectively where y is the peak area and...
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Posted by admin on Feb 1, 2014 in |
1, 5-Dihydrobenzothiazepines are synthesized by conventional and microwave assisted synthesis methods. By microwave assisted synthesis, a considerable increase in the reaction rate has been observed and that too, with better yields. The compounds have been screened for antimicrobial and cytotoxic activity. 1, 5-Dihydrobenzothiazepines are prepared by the reaction of 1, 3-diarylprop-2-enones with o-aminothiophenol. All the products were tested for purity by tlc and characterized by elemental analysis, IR, 1H-NMR, 13C-NMR and mass spectral...
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Posted by admin on Feb 1, 2014 in |
The use of medicinal plants as raw material in the production of drugs is again gaining popularity. Monechma ciliatum has many traditional uses and applications in African folk’s medicines, e.g. the seed’s powder macerated in water and drunk or burnt as an inhalation for treatment cold and allergic conditions. The aim of this study is to formulate a suitable dosage form (tablets) from Monechma ciliatum seed’s ethanolic extract. In the process of formulating of low cost, safe, effective and reproducible dosage form the wet granulation method was used. After preformulation studies, two formulae were prepared, formula-1 by using starch as a binder and disintegrant, formula-2 by using polyvinyl pyrrolidine (PVP) and cross carmellose cellulose (CCS) as a binder and disintegrant respectively. The use of starch as disintegrant in tablets of formula- 1, gave the disintegration time of 8: 33 min: sec, while the disintegration time for tablets of formula- 2 was 11: 667 min: sec by using the high cost super disintegrant CCS. Coloring agent was not needed, as...
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Posted by admin on Feb 1, 2014 in |
Natural polymer composite films from a mixture of chitosan and gelatin of various compositions were solution casted using acetic acid with and without theophylline. The films were characterized by FT-IR, TG, differential scanning calorimetry, SEM, XRD and swellability in phosphate buffer. SEM micrographs indicated uniform dispersion of the drug in the polymer blend and drug crystals were seen at higher magnification. TG, DSC and FT-IR studies implied polymer-polymer and polymer-drug weak interactions in the casted film. In vitro transdermal delivery of drug from theophylline loaded films were evaluated spectrophotometrically in phosphate buffer (pH=5.4) using Franz diffusion cell. The study revealed that at initial stage of release (upto 6h) there was only moderate change on the drug release profiles with increased content of gelatin in the film. But at higher release times (10h) the release rate was enhanced with increased gelatin-chitosan ratio. This was attributed to the improved swellability of the film in the buffer. The drug release followed a non-Fickian mechanism. The experimental observations implied that the gelatin-chitosan composite...
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Posted by admin on Feb 1, 2014 in |
Objective: To study the leaves of Simarouba glauca for their antibacterial, antioxidant, haemolytic, thrombolytic activities and to perform phytochemical evaluation. Methods: The three extracts (chloroform, methanol, ethyl acetate) of Simarouba glauca were screened for antibacterial activity against five pathogenic microorganisms by well diffusion method. In vitro antioxidant activity of extract was studied using H2O2 radical scavenging assay. The haemolytic activity was determined using agar diffusion techniques on blood agar plate, thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis. Results: Among the different extracts tested, the methanol extract of leaves showed significant antimicrobial activities. The most susceptible micro-organisms were found to be Gram negative bacteria (Stenotrophomonas maltophilia, Citrobacter), Gram positive bacteria (Enterococcus faecalis). H2O2 scavenging activity of Simarouba glauca was found to increase with increasing concentration of the extract. IC50 values of H2O2 scavenging activity was 6.72±0.1 µg/mL which was found in chloroform extract. The haemolytic activity was found to be higher in ethyl acetate extract than methanol, chloroform. The chloroform and ethyl acetate extracts shows 23.68...
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