Posted by admin on Oct 1, 2013 in |
A very simple and selective U. V. Method was developed and validated for the estimation of Atovaquone in pure form and the nanosuspension. The adequate drug solubility and maximum assay sensitivity was found in 8.00 Ph IPA Phosphate Buffer in 40:60 ratios v/v. The absorbances were measured at the λmax. of 494 nm in the wavelength range of 200-800 nm. The linear calibration range was found to be 20-140 µg/ml. The same method was applied and validated for the determination of Atovaquone in nanosuspensions. Also no interference was found from the excipients added to the nanosuspensions at the selected wavelength of 494 nm and at the analysis condition .It was found that the developed method was accurate, sensitive, precise, robust and reproducible. It can be applied directly for the estimation of drug containing the pharmaceutical...
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Posted by admin on Oct 1, 2013 in |
The aim of this study was to investigate the physicochemical properties of amlodipine besylate tablets. Tablets containing 5 mg of amlodipine besylate were prepared using Generally Regarded as Safe (GRAS) excipients. Preformulation studies were carried out using Fourier Transform Infrared (FTIR) spectroscopy and High Performance Liquid Chromatography (HPLC). Dissolution studies were performed to assess biowaiver criteria for Biopharmaceutical Classification System (BCS) Class I drug. Both the test and reference products conformed to pharmacopoeial requirements for physical parameter of tablets. FTIR studies revealed that there was no interaction between drug and excipients used in the test product. The percentage of drug remaining in the samples, subjected to the accelerated condition, complied the range specified in the United States Pharmacopeia (USP) monograph of amlodipine besylate. The dissolution of both the products was found to be more than 85 % in pH 1.2 buffer at the interval of 15 min. However, in pH 4.5 and pH 6.8 buffer media, only test product released more than 85 % drug, whereas the reference product...
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Posted by admin on Oct 1, 2013 in |
Objective: Recently, natural plants have received much attention as sources of biological active substances including antioxidants. In the present study we investigated In-vitro antioxidant activity of hexane, ethyl acetate and ethanol extracts of Solanum sisymbrifolium aerial parts. Methods: In-vitro antioxidant activity was evaluated for extracts by using free radicals Superoxide (Riboflavin photo reduction method) and DPPH (The scavenging activity for DPPH free radicals was measured according to the procedure described by Bracaand Sanja). Results: The selected plant extracts produced concentration dependent percentage inhibition of different free radicals and produced maximum activity at a concentration of 800µg and there after the percentage inhibition was raised gradually to its maximum level with higher concentrations. Conclusion: In the present study we found that the extracts of Solanum sisymbrifolium showed good antioxidant activity. Among the three extracts, hexane extract showed better activity than the other extracts on the tested super oxide free radical. The order of activity is in the following manner: Ascorbic acid >hexane extract > ethyl acetate extract> ethanol extract. Ethanol...
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Posted by admin on Oct 1, 2013 in |
Anaemia constitutes serious health problem in many countries. It is widespread public health problem, and the fourth leading cause of hospital admissions and the second factor contributing to death. Anaemia is characterized by low count of haemoglobin. The empirical use of herbal preparations in the treatment of anaemia dates from ancient times. Despite the obvious effectiveness and efficacy of iron supplementation, there are certain limitations which include gastrointestinal side effects like nausea, constipation, vomiting, and stained teeth. HgCl2 was administered for induction of anaemia in rats by oral route at 9mg/kg for 30 days simulaneousaly with test & standard drug. After 1 week blood was withdrawn to check all haematological parameters. The animals were treated with standard and test drug. After 2, 3, 4 weeks blood was withdrawn to check haematological & biochaemical parameters. Preliminary phytochemical screening by various tests was done. Acute oral toxicity was also done at 2000mg/kg and 5000mg/kg.HgCl2 induced significant decrease in the blood parameters indicating anaemia which was significantly increase in test and standard...
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Posted by admin on Oct 1, 2013 in |
Tuberculosis is one of the oldest killer diseases of mankind. It is an airborne communicable disease caused by transmission of aerosolized droplets of Mycobacterium tuberculosis. The increasing incidence of MDR- and XDR-TB worldwide highlighted the urgent need to search for newer anti-tuberculosis compounds/drugs.The World Health Organization (WHO) estimated that approximately 80% of world population relies mainly on traditional medicines, mostly plant drugs in their health care. The higher plant extracts are promising sources of novel anti-TB leads. Screening and estimation of lyophilised aqueous, aqueous ethanol and ethanolic extracts of four traditional Indian medicinal plants Acalypha indica, Adhatoda vasica, Allium cepa and Allium sativum for anti-tubercular activity tostandard strain H37Rv, clinical isolate of Isoniazid mono resistant and poly resistant isolates by the sensitive and reliable Broth Microdilution Method (BMM). Inhibition of M. tuberculosis isolates was observed for two of the four medicinal plants. All the three extracts of Allium sativum were found to be active in broth dilution assays with MIC of 500µg/ml and higher against the tested strains. The...
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