Posted by admin on Sep 1, 2013 in |
This research aims to detect the in-vitro antioxidant activity of the methanolic and aqueous stem extract of Rotula aquatica lour and to estimate its phenolic and flavonoid content. DPPH (2, 2-diphenyl-1-picryl hydrazyl), superoxide radical scavenging capacity and reducing power assay (RPA) were carried out to evaluate in vitro antioxidant activity. Phytochemical screening was carried out according to standard procedures followed by their TLC profiling was carried out. Total phenolic content was estimated by using Folin-Ciocalteu’s reagent and total flavonoid content by Aluminium chloride colorimetric method. It was observed that DPPH and superoxide IC50 value of methanolic and aqueous stem extracts of Rotula aquatica lour were found to be (50.98±0.19)µg/ml, (73.67±0.12)µg/ml and (65.41±0.42)µg/ml, (71.97±0.31)µg/ml respectively and reducing power of the extract was increased with the increasing concentration. Total phenolic and flavonoid contents were found to be 6.80 g and 5.90 g gallic acid equivalent per 100 g and 2.60 g and 1.79 g quercetin equivalent per 100 g of methanolic & aqueous extract respectively by spectrophotometric method. These primary findings...
Read More
Posted by admin on Sep 1, 2013 in |
The core moiety of ritonavir (2S, 3S, 5S)-5-(tert-butyloxycarbonyl)amino-2-amino-3-hydroxy-1,6-diphenylhexane (7) on condensation with oxirane of carbazole (8) in isopropyl alcohol at reflux and deprotected with mineral acid gave compound 10. Coupling of compound 10 with carbamate amino acid (11a-15c) in the presence of EDAC.HCl and HOBt at room temperaturegave ritonavir analogues containing carbazole (16a-20c) as a novel for the human immunodeficiency virus (HIV)-1. These compound were characterized by IR, 1H NMR and Mass...
Read More
Posted by admin on Sep 1, 2013 in |
Fioria vitifolia L. (Linn.),(Malvacae) has been extensively used in folk medicine for the treatment of common cold, flu, and upper respiratory infections and also used as an immune system booster. The methanolic extract of whole plant of Fioria vitifolia was taken underphytochemical investigation. The methanolic extract was partitioned with different solvent system by increasing their polarities using column chromatography, followed by thin layer chromatography for spot identification. Steroids, flavonoids, and triterpenes, gossypin alkaloids have been isolated from the whole part of the plant .The methanolic extract of dried plant of Fioria vitifolia (MEFV) was investigated for anti-inflammatory (carrageenan induced rat paw oedema)and anti-pyretic(brewer’s yeast induced pyrexia)and anti-oxidant activities. It is used in traditional medicine for the relief of pain and inflammation in Tamil nadu, India. Preliminary studies showed the analgesic activity of methanol extract of aerial parts of the plant against Eddy’s hot plate and tail flick models and anti-inflammatory activity against carrageenan-induced model in albino rats. In this follow up studies, the major active principle was isolated by column...
Read More
Posted by admin on Sep 1, 2013 in |
Plasmodium falciparum is the protozoan parasite responsible for the majority of life-threatening cases of human malaria, causing more than one million deaths a year. The global emergence of drug-resistant malarial parasites necessitates identification and characterization of novel drug targets. At present, α-carbonic anhydrase (CA) genes are identified in limited numbers of parasites in both protozoa and helminthes, however, the malarial genes are found in four species of Plasmodium. The CA gene of P. falciparum encodes an α- carbonic anhydrase enzyme possessing catalytic properties distinct of that of the human host CA I and II isozymes. P. falciparum native and recombinant enzymes have been prepared. Eighteen new schiff base ligand (AA01-AA18) related to previously reported potent blood schizontocidal antimalarial agent, 2-tertbutylprimaquine were synthesized and evaluated for in vivo antimalarial activities against drug-sensitive Plasmodium falciparum strain. Acute toxicity studies found that synthesized compounds were less toxic than the parent compound chloroquine, while preserving the desired antimalarial activity. Five of the analogues (AA3, AA6, AA-4, AA-9 and A-10) have exhibited curative antimalarial...
Read More
Posted by admin on Sep 1, 2013 in |
Antibacterial activity and protein content was studied in two species of octopus belonging to the family Octopoda. The highest zone of inhibition of about 34mm and 28mm were obtained against Vibrio parahaemolyticus and theprotein content was 107.16µg/L and 136.20µg/Lin Octopus dolfusii and Octopus aegina, respectively. SDS profile revealed bands of molecular weight of about 32.83 KDa to 72.36 KDa for both the species. Conclusively, the factors analyzed prove that the sample species play an important roles in chemical defensive mechanism against pathogens causing diseases in human and fishes. This study paves a way for further pharmaceutical research against pathogenic bacterial strains producing valuable...
Read More
