Posted by admin on Feb 1, 2014 in |
The objective of this study was to determine antibiotic susceptibility pattern of methicillin resistance Staphylococcus aureus in tertiary care center at Southern Rajasthan. Modified Kirby Baurer disc diffusion method was used for antimicrobial sensitivity testing of all samples. Maximum resistance among Staphylococcus aureus isolates (n=400) was observed against Methicillin (59%), Amikacin (53%) followed by other antimicrobial agents. Earlier Vancomycin, Linezolid and Pristinamycin were 100% sensitivity to MRSA. But results of this study shows that among MRSA isolates 13 % were found resistant to Vancomycin (VRSA), 12 % isolates found resistant to Linezolid and 9 % isolates Pristinamycin resistant. Pristinamycin was found sensitive in 75% of MRSA which were also Vancomycin resistant (VRSA) and Linezolid resistant (LRSA). The emergence of drug resistance and it’s dissemination in MRSA is worrisome. So we need to develop newer agents as well as slow down the spread of resistant strains by various...
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Posted by admin on Feb 1, 2014 in |
During the obtainment of herbal products, standardization of raw plant materials is ensured by the quality of herbal drugs, of the extraction conditions, followed by the determination of the content of one or more components or of the most representative compounds of their composition, which are adjusted to values previously defined, resulting in greater uniformity of plant derivatives, important for the production of safe and effective medicines. This study aimed to analyze the marker (3′, 4′, 7-tri-O-methoxyflavone) content of Peperomia pellucida L. (HBK) in three periods of collection in order to assess the seasonal changes and different extraction methods (maceration and percolation) with different solvent concentrations. The highest incidence of rain over a given period does not influence the marker content present in the sample. The maceration using 70% of ethyl alcohol obtained the highest marker content...
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Posted by admin on Feb 1, 2014 in |
Clitoria ternatea Linn is used both in traditional system and folk medicine to treat various inflammatory diseases such as arthritis and burns. The aim of the present study was to evaluate the analgesic and anti-inflammatory activity on scientific basis of different extracts of Clitoria ternatea Linnusing carrageenan-induced paw edema and tail flick method in rats respectively at various dose levels. From the results obtained among different extracts, only ethanol extract (400 mg/kg) showed maximum inhibition (23.80%) at 6th h and the early onset of action was found at 3rd h with only petroleum ether extract (400 mg/kg) showed statistical significant effect (p<0.001) compared to positive control group. Petroleum ether extracts (100 mg/kg, 200 mg/kg & 400 mg/kg) showed statistical significant inhibition from 4th to 6th h. In analgesic activity, both ethanol & petroleum ether extract showed the same type of effect but ethanol treated extract exhibited long lasting effect up to 2 h. The findings of the study proved justification of the use of the plant in the treatment...
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Posted by admin on Feb 1, 2014 in |
In the present study, different extracts of Lophopetalum javanicum (Zoll) leaves were evaluated for erythrocyte membrane stabilizing and thrombolytic activities. Phytochemical analysis of the plant was also performed to detect presence of different kinds of phytoconstituents. In order to predict anti-inflammatory activity the plant extracts were subjected to check erythrocyte membrane stabilization using heat and hypotonic solution induced hemolysis method. The crude methanolic extract, the aqueous and chloroform, pet-ether soluble fractions of L. javanicum leaves demonstrated strong membrane stabilizing activity compared to acetyl salicylic acid. In thrombolytic study, the crude methanolic extract, pet-ether, chloroform and carbon tetrachloride soluble fractions demonstrated strong thrombolytic activity in human blood specimen. Phytochemical screening revealed the presence of flavonoids, steroids, tannins and triterpenoids as major constituents in the extracts of the plant which may be responsible for the observed biological...
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Posted by admin on Feb 1, 2014 in |
An isocratic, reversed phase HPLC method was developed, using an Inertsil ODS C8 (100 x 4.6) mm,5u column and the mobile phase containing an homogenous mixture of buffer and acetonitrile (65:35,v/v). The flow rate of the mobile phase was maintained at 1.0ml/min and the detection was carried out at wavelength 250 nm. The developed method was validated with respect to linearity, accuracy (recovery), precision, system suitability, selectivity, ruggedness to prove the stability indicating ability of the...
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