Posted by admin on Nov 1, 2013 in |
Aim: To compare safety and efficacy of topical quick penetrating solution (QPS) of heparin 1000 IU/ml versus heparin gel 200 IU/g in the management of post infusion superficial thrombophlebitis. Methods: Total 202 patients of early, medium and advance stage ofsuperficial thrombophlebitis were randomized to receive either treatment. Treatments were applied 3 times daily until healing or for a maximum of 7 days. Primary efficacy endpoints were change in length of the venous lesion, proportion of patients with complete healing; while secondary efficacy endpoints included local symptoms, change in the grade of the lesion, global assessment by patients and investigator. Safety of treatment in all patients was evaluated. Results: Mean change in length of the venous lesion from baseline was significantly higher in heparin QPS group as compared to heparin gel on day 3 (P =0.0144). 90.0% patients in heparin QPS group were healed at day 7 which was significantly higher as compared to 65.7% patients in heparin gel group (P=0.00001). Heparin QPS was also found better in secondary efficacy...
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Posted by admin on Nov 1, 2013 in |
An isocratic HPLC method with UV detection at 225nm is described for simultaneous determination of Guaiphenesin, Chlor-pheneramine Maleate & Dextromethorphan HBr. Chromatographic separation of all three drugs were achieved on Purospher star RP-18e column (Merck) using Mobile phase as 74% Buffer (prepared by dissolving 1.8g dipotassium hydrogen orthophosphate in 1 litre of HPLC grade water, adjusting pH-3 with phosphoric acid) & 26% Acetonitrile. Method offers simplicity, good linearity, symmetric peak shape, and good resolution is achieved in a run time of 13 minutes. The method has been validated for Specificity, Linearity, Accuracy, and Precision. Stress testing which covered Oxidation, acid and alkali hydrolysis, photolysis and thermal degradation was performed on individual molecules under test to prove the specificity of the method and the degradation products formed were resolved effectively. It has a very good linearity with correlation coefficient value more than 0.999, % recovery for Guaiphenesin, Chlorpheneramine Maleate & Dextromethorphan HBr obtained were between 99-101%, and the relative standard deviation for 5 replicates obtained was less than...
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Posted by admin on Nov 1, 2013 in |
Two Simple, accurate, precise and sensitive spectrophotometric methods for estimation of Montelukast sodium (MON) have been developed and validated. The zero order spectroscopic method (Method I) and area under curve method (Method II) have developed. The proposed methods are validated according to ICH Q2B guidelines. The Montelukast sodium gives maximum absorbance at 241nm in chloroform and observed linearity 2-20 μg/ml for zero order method and 4-20 μg/ml for area under curve method. The stability of drug in chloroform has been studied and drug shows good stability in Chloroform. The recovery by method I and method II are 99.52 ± 0.069, 101.93± 0.11 respectively. The both spectrophotometric methods can be applied for routine analysis of Montelukast sodium in tablet formulation and in bulk...
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Posted by admin on Nov 1, 2013 in |
The present study was conducted to evaluate the hepatoprotective activity of ethyl acetate extract of stem bark of Crataeva magna (CM) in CCl4 induced toxicity in Wistar albino rats. Stem bark of CM were collected, and subjected to continuous hot extraction in a Soxhlet apparatus, for 72 h with solvents like chloroform, ethyl acetate and distilled water separately. Liver damage was induced in rats by administering CCl4 subcutaneously (s. c.) in the lower abdomen in a suspension of liquid paraffin (LP) in the ratio 1: 2 v/v at the dose of 1 ml CCl4/kg b. wt. of each animal. CCl4 was administered twice a week, on every first and fourth day of all 14 days. The extract at the dose of 200 mg/kg and 400 mg/kg b. wt. was evaluated by inducing hepatotoxicity with CCl4 and using silymarin (100 mg/kg) as the reference standard. Biochemical parameters like, SGOT, SGPT, SALP and serum bilirubin level were analysed. A section of liver was subjected to histopathological studies. Based on the above...
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Posted by admin on Nov 1, 2013 in |
In the present study, screened PGPR and Fungi were influence the growth of Mung bean (Vigna radiata) plant in the pot. Two rhizobacteria viz. Rhizobium sp., Pseudomonas putida and three fungiAspergillus niger, Rhizopus sp. and Trichoderma viride were isolated and purified. The effect of inoculation of different strains of bacteria and fungus on growth responses of Vigna radiata under pot condition was enumerated. The result revealed that the single and dual inoculation of these microbial strains enhances the plant growth in terms of root and shoot length and dry-biomass. The maximum increase in root length (up to 86.57%), shoot length (up to 56.91%), root dry weight (up to 94.42%), and shoot dry weight (up to 56.09%) was observed in response to dual inoculation of Pseudomonas putida with Trichoderma viride compared to uninoculated...
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