Posted by admin on Aug 1, 2013 in |
Now days, herbal medicines are widely used to treat, manage and cure the kidney diseases as well as reduce uremia, acute nephritis & nephritic syndrome. The present study was to evaluate the antiuremic and antioxidative effect of Asparagus racemosus (AR) on acetaminophen induced uremic male rats. The study was designed with 36 male albino rats which were randomly divided into 6 groups. Group I animals were provided normal food and water ad libitum, Group II, III, IV, V and VI received acetaminophen intraperitoneally at the dose of 500 mg/kg body weight/day for 10 days. Methanol, aqueous, hydromethanol, and hexane extract of AR at the dose of 500mg/kg body weight/day were fed orally on the 11th day and continue for next 15 days to group III, IV, V and VI respectively. After 25 days, group-II animals showed significantly increased (p<0.05) plasma urea, creatinine, sodium. Elevation of lipid peroxidation was noted by measuring Malondialdehyde level in both plasma and kidney tissues than Group I, III, and V. Plasma Potassium, plasma and...
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Posted by admin on Aug 1, 2013 in |
Nicorandil is occasionally given for cardiovascular disorders in patients also suffering from diabetes mellitus. Simultaneous use of nicorandil and sulfonylureas may interfere with the euglycemia. The study was done to find the effect of nicorandil on glucose tolerance in normal male Wistar albino rats, under oral glucose load, adrenaline injection and in receiving oral hypoglycemic drugs. Male albino rats 100-120g was divided into control and treatment groups. The control group of rats received either oral 2% gum acacia or normal saline subcutaneously. The treated groups received either glucose (1g/100g), glipizide (0.045mg/100g) or nicorandil (0.18mg/100g) orally alone or in combination. Blood samples were collected from lateral tail vein at 0, 1, 2 and 4hrs interval. Blood glucose estimation revealed that nicorandil significantly reduced glucose tolerance in normal rats and in rats made hyperglycemic by oral glucose load or adrenaline injection. It antagonized the hypoglycemic effect of glipizide and reduces glucose tolerance in rats at human therapeutic...
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Posted by admin on Aug 1, 2013 in |
Esomeprazole is used to treat gastro esophageal reflux disease. Naproxen is a Non-steroidal anti-inflammatory drug (NSAID) used in the treatment of pain or inflammation caused by conditions such as arthritis, ankylosing spondylitis, tendinitis, bursitis, gout, or menstrual cramps. A simple, precise, cost effective RP-HPLC method was developed and validated for the determination of both Esomeprazole and Naproxen in Pharmaceutical compositions. The chromatographic separation was achieved on a Symmetry C18 (4.6 x 150mm, 5mm, Make: XTerra) using a mobile phase consist of a mixture of phosphate buffer (pH 3) and Acetonitrile [60: 40]. The flow rate of mobile phase was maintained 1.0 mL per minute. The wavelength chosen for detection was 285 nm. The retention times of Esomeprazole and Naproxen peaks were around 2.105 and 3.555 mins respectively. The Accuracy was calculated for 50%, 100% and 150% and the % recovery was found to be 98.0%-100.4%. The method was found to be linear over the range of 5ppm to 9ppm per mL for Esomeprazole 125ppm to 225ppm per mL for...
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Posted by admin on Aug 1, 2013 in |
A Streptomyces strain was isolated from soil and was identified as Streptomyces spectabilis on the basis of 16 S rRNA gene sequencing. On screening for its ability to produce antimicrobial compounds, it was found to be active only against Gram positive bacteria. Attempts were made to optimize the culture conditions for the production of antimicrobial compounds. Basal medium supplemented with glucose as a carbon source was found to be the best for growth but not for antibiotic production. Antibiotic production proved to be highest in the medium amended with cellobiose. Jack bean meal (JBM) and peptone were the best nitrogen sources for growth and antibiotic production respectively. Antibiotic production appeared to start after 48 hours and reached a maximum on the fourth day. Maximum growth and antibiotic production occurred when the initial pH was adjusted to...
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Posted by admin on Aug 1, 2013 in |
Pyogenic infection refers to bacterial infection that leads to the production of pus. Antibiotics to treat these pyogenic bacterial infections are routinely prescribed, toxicity of which is serious threat and makes chemotherapy more difficult. Management of pyogenic infections consists of aspiration or surgical drainage followed by appropriate antibiotics. A total of 50 samples were examined, 36 bacterial strains were isolated, 20 Gram positive, Staphylococcus aureus (8), Staphylococcus epidermidis (7), Corynebacterium pyogenes (5) and 16 Gram negative, Escherichia coli (4), Pseudomonas aeruginosa (4), Neisseria meningitidis (4). Morphological and biochemical tests confirmed the isolated microorganisms. The study was carried out using in vitro Kirby Bauer’s disk diffusion method. The percentage resistance of different Gram positive isolates against different antibiotics, penicillin, amoxicillin, ofloxacin, cefazolin, cefuroxime, erythromycin, chloramphenicol, ciprofloxacin, azithromycin and tetracycline was C. pyogenes (76%), S. aureus (61.25%) and S. epidermidis (48.55%). Results for Gram negative bacteria, N. meningitidis (62.5%), E. coli (61.6%) and P. aeruginosa (52.28%), against norfloxacin, gentamycin, ofloxicin, cefixime, cefuroxime, amicacin, nalidixic acid, cefotaxime and ceftriaxone were also observed....
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