Posted by admin on Jan 1, 2014 in |
ABSTRACT: Stress exerts detrimental effects on several cellular functions through impairment of antioxidant defences, leading to oxidative damage and onset of many cardiovascular and neurological diseases. The restraint stress or immobilization stress is widely accepted model to induce emotional stress and the deleterious effect of restraint stress on the biological system is well documented. The precise molecular and cellular events induced by restraint stress needs to be evaluated. Aim: To determine the effect of sub-acute restraint stress on antioxidant status in rats. Methodology: Adult male Wistar albino rats weighing about 180-200g were taken for the study and was divided into two groups – Control group (n=6) and Sub-acute restraint stress group (n=6). Restraint stress were given in wire mesh restrainers for 15 days (6 hrs/ day), the blood from the jugular vein was collected for estimation of antioxidant status (SOD, GPx, CAT, Vit C and Vit E) in rats. Results: After 15 days of restraint stress, there was a significant (P<0.05) increase in LPO in restraint stress when compared...
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Posted by admin on Jan 1, 2014 in |
Microspheres are the carrier linked drug delivery system in which particle size ranges from 1-1000μm. Microspheres constitute an important part of drug delivery systems by virtue of their small size and reliable means to deliver the drug to the target site with specificity. However, the advantages of microspheres are limited due to their short residence time at site of absorption. This can be achieved by coupling bioadhesion characteristics to microspheres and developing “mucoadhesive microspheres”. Glipizide is an oral hypoglycemic drug with short half-life. So to provide prolonged action glipizide microspheres were prepared by ionic gelation technique using different concentration of mucoadhesive polymers. The prepared microspheres were characterized for various physicochemical prarameters such as particle size, percentage yield, encapsulation efficiency, scanning electron microscopy, in-vitro drug release and kinetics study. The in-vitro drug release studies were performed in buffer media and shown the controlled release pattern of drug up to 10 hours. Drug release from the microspheres was found following zero order release kinetics with non-fickian release mechanism. The satisfactory results...
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Posted by admin on Jan 1, 2014 in |
A food borne illness or disease is exactly what the term indicates a disease or illness caused by the consumption of contaminated foods or beverages. It would seem rather obvious that a food borne microbial pathogens, or a microbial toxic product, or another poison such as a poisonous chemical that has somehow contaminated the food and beverages leads to one of the many different food borne illness. Many microorganisms present are associated with infection, spoilage and contamination of food in home, restaurants, dhabas and other various sites. We collected and isolated bacterial and fungal strains by various morphological characteristics, biochemical identification etc. Disc diffusion method was used to study antibiotic resistant capability against infectious microorganisms. In the antibiotics Kirby Bauer (disk diffusion) test only 3 tested S. aureus displayed a inhibition zone against some antibiotic, and they resistant against by Amoxicillin, Oxacillin, Ciprofloxin, but sensitive to Doxycycline, Erythromycin. We tested Bacillus subtilis and Pseudomonas also by antibiotic disc diffusion method and observation of investigators in which only few antibiotics...
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Posted by admin on Jan 1, 2014 in |
Olanzapine is a thienobenzodiazepine class of drugs, which has been approved by the Food and Drug Administration (FDA), for the treatment of schizophrenia, depressive episodes associated with bipolar disorder, acute manic episodes, and maintenance treatment in bipolar disorder. Recently many formulations developed such as parenteral formulations to improve compliance in the treatment of schizophrenia and to treat agitation in patients with schizophrenia and bipolar mania. Olanzapine palmate long acting injection (depot) is a novel formulation of the atypical antipsychotic Olanzapine. The aim of the study is to prepare tablet with similar elegancy, therapeutically effective, bioequivalent formulations to that of zyprexa. Here Olanzapine tablets were prepared by wet granulation method using following ingredients: Lactose monohydrate, Mannitol, L-HPC, Microcrystalline cellulose, Crospovidone, HPC EX, HPC LF, Magnesium Stearate, HPMC, Poloxamer, pectin. The tablets were evaluated for weight variation, thickness, hardness, friability, disintegration, dissolution and stability study for the best formulation F10 which is having similar release profile as that of zyprexa. Content uniformity of F10 formulation is 99-99.6%. Total impurities for the...
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Posted by admin on Jan 1, 2014 in |
The aim of the study was to evaluate the effect of Musa sapientum methanolic fruit extract on volume of gastric juice and pepsin activity in aspirin induced peptic ulcer in albino rats. The extract was prepared by percolation method. Volume of gastric juice was estimated by the method of Deshpande SS et al (2003). Pepsin activity was studied with the methods of Debnath PK et al (1974) and Lowry OH et al (1951). Twenty four healthy albino rats of 100-200 gms were divided into 4 groups of six animals each. Group I or control (3% gum acacia, 5 ml/kg orally for 7 days). Group II or experimental control (aspirin 400 mg/kg orally single dose on the 7th day). Group III or MSE group (MSE 100 mg/kg orally for 7 days and aspirin 400 mg/kg orally single dose on the 7th day). Group IV or standard (ranitidine 150 mg/kg orally for 7 days and aspirin 400 mg/kg orally single dose on the 7th day). On 8th day the stomachs of...
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