Posted by admin on Sep 1, 2013 in |
Plants have been used as a source of natural products for various therapeutic processes from a long time. The proposed work illustrates the study of the importance of natural products in terms in-vitro approaches. On account of this study the phytochemical constituents of various extracts of Hemigraphis colorata (Blume) are evaluated. The plant was traditionally known for its wound healing activities. Crude plant extract was prepared by percolation method using a Soxhlet apparatus. The solvents used were Hexane, Chloroform, Acetone and Ethanol .The in-vitro antioxidant capacity was analysed through DPPH and reducing power assays. Anti-inflammatory properties of various extracts from the whole plant are analyzed through human RBC membrane stabilisation method. The anti-oxidant and anti-inflammatory activities were more for the ethanolic extracts of Hemigraphis colorata when compared to acetone, chloroform and hexane extracts.The in-vitro cytotoxicity analysis of ethanolic extract was also carried out in the present study using trypan blue...
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Posted by admin on Sep 1, 2013 in |
Back ground: Alcoholic cirrhosis is a condition of irreversible liver disease due to the chronic inflammatory and toxic effects of ethanol on the liver. Acute alcoholic hepatitis occurs in habitual drinkers, often a period of increased alcohol intake. Although, the clinical features may mimic acute viral hepatitis. Progression of liver fibrosis to cirrhosis is dangerous as well as potentially life-threatening condition that occurs when inflammation and scarring damage. Several biochemical parameters are altered in this condition .A combination of tests are useful for diagnosis of cirrhosis. Aim & objective: The aim of the study evaluates the possible association between the acute phase proteins along with other biochemical parameters in alcoholic liver disease. Materials and methods: A total 30 chronic alcoholic liver disease patients between the ages of 32 and 60 years are selected for the study group, 30 healthy age matched healthy individuals are selected as a control group. All the parameters are analyzed by conventional standardized methods and compared between the two groups. Results: The mean levels of...
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Posted by admin on Sep 1, 2013 in |
Background: Medicinal plants in Tripathi hills and other developing countries have been scientifically demonstrated to have medicinal benefits but few or none have been translated to products for clinical use. Most herbal products developed by local herbalist and sold to the public are not standardized and lack efficacy and safety data to support use. Objective: to evaluate the medicinal plants for its wound management and test its preclinical safety and efficacy using rat models. Methods: Thirty Wistar albino rats were randomly divided into five groups and wounds were surgically created on the mid dorsal region. The wounds were treated topically and group I served as control group administered with distilled water, group II served as standard, Group III served with MESP, group IV treated with MESI and the group V was treated with METR. The effects of the treatment on the rate of wound closure were assessed. Results: the plant extracts had significantly higher rate of wound closure which was better than distilled water. The significancy was found to...
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Posted by admin on Sep 1, 2013 in |
Nomuraea rileyi (Farlow) Samson is an entomopathogenic fungus capable of producing a variety of enzymes including proteases and chitinases. Protease derived from microorganisms such as fungi, bacteria, and yeast has established wide spread applications in fields such as in the food, detergent and other industries. This study investigates the strategy for partial purification and characterization of protease enzyme from Nomuraea rileyi. The fungus was primarily screened on skim milk agar plate and a clear zone of skim milk hydrolysis confirmed the protease production. The protease activity of 89 U/100 ml was observed in crude sample and then subjected to partial purification by ammonium sulphate precipitation followed by dialysis. The protease enzyme was partially purified to 3.64 fold with a yield of 3.5%. The partially purified protease exhibited optimum activity at pH 8.0 and temperature at 40ºC. Proteases can be used for various commercial purposes such as detergent, waste treatment, leather industry for dehairing the animal skins and degradation of natural proteins. In the present study protease were tested upon...
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Posted by admin on Sep 1, 2013 in |
Quantitative and qualitative improvement of rare and valuable bioactive plant products is promisingly possible using In-vitro cell culture techniques. The present investigation was conducted with the aim to elicit the synthesis ofandrographolide by subjecting cultured cells of Andrographis paniculata to Salicylic acid (SA) and Chitosan mediated abiotic stressCell suspension culture of Andrographis paniculata (Burm. f.) Nees. was treated with salicylic acid and chitosan at late exponential phase. The results were analyzed using High Performance Liquid Chromatography technique. The suspension culture was treated with 0.05mM, 0.5mM and 1.5mM concentrations of salicylic acid for 24, 48 and 72 hrs of treatment duration. The treatment of chitosan was administered to the cell suspension of Andrographis paniculata with the concentrations of 5 mg, 10 mg and 20 mg per 50 ml of suspension culture and each concentration was studied for 24, 48 and 96 hrs of treatment duration. The results revealed that salicylic acid (0.05 mM) for 24 hrs of treatment duration showed as maximum as 18.5 fold increment in andrographolide content (37.0...
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