Posted by admin on Aug 1, 2013 in |
Use of plants as a source of traditional healing systems around the world that utilize herbal remedies is an important source for the discovery of new antimicrobials against resistant strains of bacteria. It is being used as a source of medicinal agents for antibacterial, antihelminthic, astringent, emetic, sedative and stimulant. A 10 g dried leaves powder of Woodfordia fruticosa was extracted separately in each different solvent i.e., aqueous, ethanol and methanol. The extract was stored at 4ºC in airtightbottles until further uses. Total 8 strains including gram positive and gram negative bacteria were selected to assess the susceptibility test against the different drug extract. These are Escherichia coli (ATCC-25922), Pseudomonas aeroginosa (ATCC-27853), Salmonella paratyphi (ATCC-9150), Salmonella typhimorium (ATCC-25241), Shigella sonnei (ATCC-25931), klebseilla pneumonia (ATCC-27736), Staphylococcus aureus (ATCC-25923) and Proteus vulgaris (ATCC-6380). The antibacterial activity was performed in vitro using Agar well diffusion assay and diameter of zone of inhibition was measured. The Methanolic shows good zone of inhibition in almost bacteria. The highest zone of inhibition was observed in...
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Posted by admin on Aug 1, 2013 in |
The rapid and sensitive UV spectrophotometric method was described for the determination of etodolac, non-steroidal anti-inflammatory drugs. The type of solvent, the range of wavelength and the range of concentration were chosen in order to optimize the conditions. The developed and validated method was successfully applied to the analysis of etodolac without derivatization with any compound in commercial formulations. The standard calibration curve of etodolac was constructed by plotting absorbance versus concentration in the determined concentration range with the final dilution. The calibration curve was Absorbance in 277 nm wavelength = 0.0303 Concentration in ppm –0.0201 and linear (R³0.9995) in concentration range of 1-50 ppm of etodolac. The LOD and LOQ values of method were 0.7 ppm and 1.0 ppm, respectively. Moreover, the proposed method was fast with respect to analysis time as compared to more sophisticated chromatographic...
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Posted by admin on Aug 1, 2013 in |
Purpose: The current investigation was carried out to study the hematopathological changes in the developing red blood cells of neonate of Swiss albino mice exposed with lead acetate and vitamins. Methods: Lead acetate was administered orally at 8, 16, 32 mg /kg/BW to selected pregnant females from 10th day of gestation to 21st day of lactation. Vitamin C (166 mg/kg BW) and vitamin E (133mg/kg BW) individually and in combination with lead were also administered from 10th day during the same period. Hematopathological alterations in the RBCs were examined in neonates after birth at postnatal days 1, 7, 14 and 21. Results: The examination of blood smears demonstrated that lead exposure during gestation and lactation led to various hematological disorders in red blood cells of neonates and abnormal types of RBCs like schistocytes, stomatocytes, codocytes, echinocytes with prominent hypochromasia, anisocytosis, macrocytosis etc. were also noticeable. The antioxidants vitamin C and E also induced structural abnormalities independently and also with co-administration of lead in developing RBCs in forms of dacrocytes,...
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Posted by admin on Aug 1, 2013 in |
A simple, sensitive, rapid, accurate and economical polarimeteric method has been developed and subsequently validated for simultaneous determination of Sucrose – in Iron Sucrose Raw material, Iron Sucrose Injection and Inprocess Bulk sample of Iron Sucrose formulations in pharmaceuticals. The usual measure of purity of sucrose is by polarimetry – the measurement of the rotation of plane-polarized light by a solution of sucrose. The specific rotation at 20°C using yellow “sodium-D” light (589 nm) is +66.47°. Commercial samples of sucrose are assayed using this parameter. Readings are not affected by the molecular weight of the Iron Sucrose, the entire procedure takes much less time to perform. The developed method was compared with RP-HPLC method. The described method was linear over a concentration range of 10-70 mg per ml for the assay of Sucrose. Results of analysis were validated statistically and by recovery studies. The limit of detection (LOD) and the limit of quantification (LOQ) for Sucrose were found to be 0.076 and 0.231mg per ml. The results of the study...
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Posted by admin on Aug 1, 2013 in |
Vascular oxidative stress occurs in hypertensive states and the potential role of antihypertensive drugs as antioxidants is currently under review. Hydrogen sulfide (H2S) is a recently recognized member of gasotransmitters, which may act to decrease blood pressure in experimental hypertension. The present study evaluated the antioxidant potential of hydrogen sulfide in SHR. NaHS, donor of H2S, was subjected to a series of in vitro tests to evaluate its antioxidant capacity. For in vivo study Wistar Kyoto (WKY) and spontaneously hypertensive rats (SHR) were divided in 4 groups namely WKY control (I), WKY-NaHS treated (II), SHR control (III) and SHR-NaHS treated (IV). Groups II and IV received NaHS, 56 µM/kg i.p. daily for 4 weeks. Blood pressure, renal cortical blood perfusion and pulse wave velocity were measured in acute studies. Oxidative and antioxidant markers from plasma were measured at the end of 4 weeks. In vitro, NaHS was found to be a free radical scavenger, reductant and inhibitor of lipid peroxidation. In vivo, the SHR control rats had higher blood...
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