Posted by admin on May 1, 2013 in |
Aim: To investigate the possible association of OCT1 rs122083571 polymorphism (C/T allele) with glycemic status and serum metformin levels in type II Diabetes mellitus. Methods: Sixty already diagnosed T2DM patients on metformin monotherapy (500 mg, bd) were recruited for the study and the genotypes for SLC22A1 rs122083571 C/T polymorphism using PCR assay were done. Serum fasting plasma glucose (FPG), Postprandial Plasma Glucose (PPG), Glycated Hemoglobin (HbA1c), Fasting Serum Insulin (FINS), Triacylglycerol (TG), Cholesterol (CHO), Low-Density Lipoprotein (LDL-c), High-Density Lipoprotein Cholesterol (HDL-c), and Homeostasis Model Assessment for Insulin Resistance (HOMA-IR), Body Mass Index (BMI), blood pressure (BP) were determined. Results: In patients with C allele, the BMI,BP ,FPG ,PPG and Insulin levels were significantly higher when compared to that of the T allele subjects .The values of TGL and HDL in both groups of T2DM patients were not significantly different. But the total cholesterol level and LDL level were significantly increased in T2DM patients with C allele. Conclusion: SLC22A1 rs122083571 C allele is associated with poor glycemic status in spite...
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Posted by admin on May 1, 2013 in |
Various locally available vegetables were screened for the enzyme peroxidase. The screening was performed mainly on daikon, cabbage, cauliflower; tomato and sweet potato etc. The juices of these vegetables were extracted and subjected to ammonium sulphate precipitation followed by dialysis to remove any adhere salts. The partially purified enzymes were then subjected to peroxidase assay. All the extracts from vegetables showed the presence of appreciable amount of peroxidase. In order to quantify, kinetic measurements of all the partially purified enzymes were performed using hydrogen peroxide as substrate and guaiacol (ortho-methoxy phenol) as color forming dye. The enzymatic activities of daikon, cabbage, cauliflower, sweet potato and tomato peroxidase having optical densities of 0.056, 0.139, 0.226, 0.139 and 0.089 at 280 nm were found to be 2.2898 U/ml, 3.4381U/ml, 1.1675 U/ml, 0.3741 U/ml and 0.1901 U/ml respectively. In order to compare the above result, the data have been normalized with respect to optical densities of cabbage. Thus, the partially purified enzyme having a optical density of 0.139 shows the peroxidase activity...
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Posted by admin on May 1, 2013 in |
The present study was carried out to determine the presence of phytocomponents in the aerial parts of Cleome burmanni using Gas Chromatography- Mass Spectrometry (GC-MS) analysis. The mass spectrum of the crude chloroform extract was compared with the available library sources (NIST08 LIB, WILEY8 LIB). Sixteen components were identified. Sterols, terpenoids and hydrocarbons were the most prominent. The major components detected were 24(s)- Ethyl-3- alpha, 5-alpha-cyclocholest-22 (E)-en-6-one (19.29%), Delta-4-Sitosterol-3-one (14.75%), Cholest-4-en-3-one (12.35%), Stigmasta-5, 23-Dien-3-ol, (3-beta) (12.17%), Neophytadiene (6.83%), Hexatriacontane (6.22%), 1-alanine, N-(3-fluorobenzoyl) – undec-10-enyl ester (5.96%), phytol (5.67%), tetracontane (4.30%), 1,2- benzenedicarboxylic acid (3.25%). The other components were present in minute...
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Posted by admin on May 1, 2013 in |
Background: Maternal mortality is one of the indicators in the millennium development goal that is raising concern in achieving the set target of reducing the rate by three-fourth by 2015. Women who die due to pregnancy-related causes are in the prime of their lives and are responsible for the health and well-being of their families. Objective: To assess patterns of maternal mortality and associated factors: at public hospitals in Tigray region, Ethiopia, 2010-2012. Methods: A hospital based case control study was done from May 2012 to December 2012 at public hospitals found in Tigray regional state. Cases were mothes who died in hospital between July, 2010 to June, 2012 and controls were mothers who gave birth in the same hospital during same period. Simple random sampling method was used to select the case and controls. A ratio of four controls to one case was considered to assure sufficient sample size. Data were entered, cleared and analyzed using SPSS window 16. Bivariate analysis was used to determine an association between...
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Posted by admin on May 1, 2013 in |
In the present study an attempt has been made to prepare fast dissolving tablets of Sumatriptan Succinate to improve dissolution rate of the drug in oral cavity & hence better patient’s compliance & effective therapy. Sumatriptan Succinate is a 5-HT1D (5-hydroxy tryptamine 1D) receptor agonist, used in the treatment of migraine and cluster headache. It has low bioavailability due to its first pass metabolism. Hence the main objective of the study was to formulate fast dissolving tablets of Sumatriptan succinate to achieve a better dissolution rate and further improving the bioavailability of the drug. Fast dissolving Sumatriptan succinate was prepared using superdisintegrants Crospovidone, Croscarmellose Sodium, and Preglatinised Starch using the direct compression method. The tablets prepared were evaluated for Thickness, Uniformity of weight, Drug content, Hardness, Friability, Wetting time, in vitro and in vivo disintegration time, in vitro drug release. The formulation F3 containing Crospovidone (8%) was found to give the best result since it showed enhanced dissolution, which leads to improved bioavailability, improved effectiveness and hence better patient...
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