Posted by admin on Aug 1, 2013 in |
A simple HPTLC method has been developed for the estimation of Gallic acid and Piperine in methanolic extract of Zeal herbal granules [ZHG], a Polyherbal formulation. The separation was performed on TLC aluminum plates precoated with silica gel 60 F254, good separation was achieved in the mobile phase of toluene: ethyl acetate: formic acid (11: 15: 1v/v/v) and densitometry determination of Gallic acid (0.50 Rf) and Piperine (0.70 Rf) was carried out at single wavelength scanning at 254 nm. The method validated as per ICH guideline, shows regression co-efficient value for Piperine 0.9992 and 0.9979 for Gallic acid in range of 40-90 µg/mL Recovery of Piperine and Gallic acid in final product found in range of 98-102%. Precision study (interday & intraday) showed that the relative standard deviation is less than 2%, showing method is well precise. Quantification study shows 0.23 % w/w of Piperine and 0.37 % w/w of Gallic acid in ZHG. Present study shows that developed method is simple, precise, specific, robust and accurate, and could...
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Posted by admin on Aug 1, 2013 in |
The critical nature of pharmaceutical dosage forms requires that both formulation and process variables are optimized in order to achieve desired quality outcome. In this study, process optimization is examined with a view to position the final product in such a way as to deliver the expected performance. Six formulations were designed with similar excipients and nifedipine as model drug. Each formulation went through wet granulation process, during which the granulation time, amount of granulation liquid and wet milling using different sieve sizes were modified to reflect the parameters being investigated. Results vindicated the importance of modification of process variables to the extent that significant difference was observed in the physicochemical parameters, especially in vitro dissolution and drug release rates. Data from in vitro dissolution testing was fitted into different kinetic models so as to predict the order and mechanism of drug release. It could be concluded from this research work that through logical process optimization, it is possible to identify critical process parameters that can be modified to...
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Posted by admin on Aug 1, 2013 in |
Aim: To evaluate the in vitro antioxidant, antimutagenic and cytotoxic property of hydro-alcoholic extract of Thymus vulgaris L. by subjecting it to free radical scavenging, Ames and MTT assay respectively. Methods: Powdered Thyme leaves were extracted in a Soxhlet apparatus using 70% ethanol to obtain a dry extract. The extract was assessed for its total phenolic, tannin, flavonoid and flavonol content using quantitative assays. The antioxidant potential of Thyme was determined using DPPH•, ABTS•+, FTC and metal chelating assays. The in vitro inhibitory effects of Thyme on lipid peroxidation of liver and kidney along with erythrocyte hemolysis were performed following standard procedures. Further, the antimutagenic property of Thyme was investigated using Ames assay against TA 98 and TA 100 mutant strains of Salmonella typhimurium. The in vitro cytotoxicity assay of Thyme was examined following the MTT assay against Hep 2 and Hep G2 cell lines. Results: The hydro-alcoholic extract of Thyme registered 91.42 ± 0.90, 63.23 ± 1.02, 55.01 ± 0.42 and 65.69 ± 0.43 mg/g extract of total...
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Posted by admin on Aug 1, 2013 in |
Objectives: Arformoterol is the (R, R) – enantiomer of formoterol, a short and long acting β-2 agonist bronchodilator and is distinguishable from the racemic (R, R/S,S) – diasteriomer of formoterol, which is most commonly used. Some in vitro data show significant bronchodilatation, inhibition of inflammation and marked baseline airway reversibility with arformoterol. Yet, there is a dearth of study literature demonstrating its role as immediate reliever medication, in patients with exacerbation of non-severe asthma. The aim of this study was to compare the efficacy and safety of arformoterol with salbutamol nebulisation therapy, as a rescue medication, in acute non-severe asthma, not requiring hospitalisation, at a tertiary care centre. Methods: 40 patients, with exacerbation of non-severe asthma, were randomly allotted to Group A=20 and Group B=20. After every 30 minutes, 3 doses of salbutamol or arformoterol nebulization were given, respectively. Peak expiratory flow rates (PEFR) were measured at the baseline and 6 min, after each dose. Results: In the demographic profile, there were comparable results, with no statistically significant difference...
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Posted by admin on Aug 1, 2013 in |
Ethanol extract of the leaves of Taxus baccata, Phyllanthus debilis, Plectranthus amboinicus were studied for their antimicrobial property against multidrug resistant bacterial strains (MDR) from clinical specimens. Of these three plants, the most potent antimicrobial plant was Phyllanthus debilis followed by Taxus baccata (MIC range 80 – 400μg/ml) having broad spectrum activity, whereas Plectranthus amboinicus had no effect against MDR isolates. Taxus baccata and Phyllanthus debilis can be used as antimicrobial agents. Further confirmation needs ethano-pharmcological...
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