Posted by admin on Jul 1, 2013 in |
Aims: To study effect of piracetam in experimental models of depression. Methods: Mice (n = 6/group) pretreated with distilled water, fluoxetine (28 mg/kg) and piracetam – 100, 200, 300, 400, 500, 750 and 1000 mg/kg for 7 days were subjected to tail suspension test (TST) on day 7. Rats (n=6/group) were pretreated for 7 days with distilled water, fluoxetine (20 mg/kg) and piracetam (300, 400, 500, 750 and 1000 mg/kg) and on day 7, forced swim test (FST) was conducted. Immobility time in seconds was noted in both the models and analysed using one-way ANOVA (level of significance p<0.05). Results: In TST, immobility time was reduced significantly (p<0.01) and in a dose-dependent manner with all but one dose of piracetam compared to vehicle. In FST, significant difference from control group was seen with higher doses of piracetam (500, 750 and 1000 mg/kg) with a dose-dependent trend. The mean immobility durations in the groups with significant improvement were found to be comparable to that of the respective fluoxetine groups in...
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Posted by admin on Jul 1, 2013 in |
A simple, rapid, precise and accurate isocratic reversed-phase stability-indicating HPLC method was developed and validated for the simultaneous determination of Atazanavir (AT) and Ritonavir (RT) in commercial tablets. The method has shown adequate separation for AT, RT from their degradation products. Separation was achieved on a Hypersil BDS-C18, 5 μm, 125 mm × 4.6 mm i.d. column using a mobile phase consisting of buffer (pH3.4) – acetonitrile (50:50, v/v) at a flow rate of 1.5 mL/min and UV detection at 250 nm. The drugs were subjected to oxidation, acid, base hydrolysis, photolysis and heat to apply stress conditions. The linearity of the proposed method was investigated in the range of 7.8–225 μg/mL (r2 = 0.9993) for AT and 2.7-75 μg/mL (r2 = 0.9995) for RT. The limit of detection was 2.4 μg/mL for AT and 0.9 μg/mL for RT. The limit of quantitation was 7.8 μg/mL for AT and 2.7 μg/mL for RT. Degradation products produced as a result of stress studies did not interfere with the detection of AT and RT and the assay can thus be considered...
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Posted by admin on Jul 1, 2013 in |
In this research work we elucidated the anti-inflammatory and anti-arthritic activity of hydroalcoholic extract of Asparagus racemosus roots (ARHE) in-vivo. Carrageenan induced paw edema methodology is used to induce inflammation and Diclofenac sodium is used as standard drug whereas Freund’s Complete adjuvant used to induce arthritis and Dexamethasone is used as standard drug. Asparagus racemosus rootswere showed significant anti-inflammatory and anti-arthritic activity at oral dose of (200mg/kg and...
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Posted by admin on Jul 1, 2013 in |
An experimental study was carried out to select a suitable complex nutrient medium for growth and As(III) biosorption by an As(III) resistant strain Aspergillus niger X300 .For this purpose different complex nutrient were examined one by one. The recommended complex nutrients (gm%) were corn steep liquir, 20; rice bran extract, 10; paddy soak liquir, 10; wheat bran extract, 15 beef extract, 30, meat extract, 2 0 potato scale extract, 15 and malt extract,...
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Posted by admin on Jul 1, 2013 in |
Nanotechnology is widely used for delivery of various drugs to the body for increasing the bioavailability, reducing toxicity and controlled release.Nanospheres are the solid particles which are spherical in shape, having the particle size range between 1-1000 nm and having better bioavailability. In nanospheres, the drug is physically and uniformly dispersed in the matrix system of the polymer. Nanospheres of Propranolol Hydrochloride were prepared by using solvent evaporation technique with different concentration of Eudragit RS100 and Eudragit RL100 polymers. The different formulation factors like drug: polymer ratio, concentration of solvent, stirring speed, stirring time on particle size, drug encapsulation, drug efficiency, surface morphology, and process yield and drug release behavior was studied. The in-vitro performance of nanospheres were evaluated by recovery efficiency, particle size analysis, surface topography (using scanning electron microscopy), drug-polymer compatibility (using differential scanning calorimetry) and drug release studies. The single emulsion solvent evaporation method used for nanospheres preparation was suitable in the particle size range between 265.67±3.98 nm, the encapsulation efficiency was 74.67±2.56% (w/w) and the...
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