Posted by admin on May 1, 2013 in |
Objective: To formulate and evaluate the Levosalbutamol sulphate loaded microspheres of Chitosan and microspheres of Flax seed Mucilage in which both the polymers were checked as potential mucoadhesive agents and crosslinking was done with Glutaraldehyde for prolonging drug release for asthma therapy. Material and Method: Chitosan and Flax seed mucilage (by drying in Hot Air Oven) were used in appropriate concentration and crosslinked microspheres were prepared by Spray drying. They were evaluated for %Entrapment efficiency, Swelling behavior, %Mucoadhesion and In-vitro drug release. The optimized batches were subjected to Particle-size distribution, Scanning-electron microscopy, Aerosolization behavior and Stability study. Result and Discussion: Microspheres having Chitosan polymer: Drug ratio of 2:1 and 0.6% of Glutaraldehyde and that of Flax seed Mucilage: Drug ratio of 3:1 and 0.9% of Glutaraldehyde had good mucoadhesion ability (89.50% and 85.11% respectively). Release rate was retarded up to 8 h and follows non-fickian diffusion. Particle-size distribution of optimized batches was represented by span index (3.822 and 2.953 respectively) and value was nearer to marketed product (1.975). Aerosolization...
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Posted by admin on May 1, 2013 in |
In the present study, Miglitol 25 mg controlled release matrices were prepared by direct compression and in vitro drug dissolution studies were performed to find out the drug release rate and patterns. Hydroxypropylmethylcellulose, Hydroxypropylcellulose and Hydroxyethylcellulose were used as rate controlling polymers. Hydroxypropylmethylcellulose was used as primary rate controlling polymer and effects of addition of Hydroxypropylcellulose and Hydroxyethylcellulose on in-vitro drug dissolution were studied. Tablets were formulated using total polymer content as 30, 35 and 40 percent with 20 percent standard polymer content of Hydroxypropyl methylcellulose in all batches and varying the concentration of Hydroxypropyl cellulose and Hydroxyethylcellulose in the range of 10, 15 and 20 percent. In-vitro drug release was carried out using USP Type II at 50 rpm in 900 ml of acidic dissolution medium (pH 1.2) for 2 hours, followed by 900 ml alkaline dissolution medium (pH 7.4) up to 12 hours. Several kinetic models were applied to the dissolution profiles to determine the drug release...
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Posted by admin on May 1, 2013 in |
The present study was conducted to perform the translation and validation of Multi-Dimensional Questionnaire (MDQ) instrument for use in Indian type 2 diabetic patients. Validated English version ofMDQ instrument was selected for the study. The instrument was subjected to forward and back translation to generate final version in the Hindi language. The instrument consists of three sections: Section I, general perceptions of diabetes and related social support measuring interference, severity and social support. Section II, social incentives related to self-care activities measuring positive reinforcing behaviors and misguided reinforcing behaviors and Section III, self-efficacy and outcome expectancies measuring self-efficacy and outcome expectancies. Validation was done in two hundred fifty Indian diabetic type 2 patients after the pilot testing (n=50). Internal consistency was assessed using Cronbach’s alpha and value of 0.84 was gained for the summary score and alpha values of 0.81, 0.93 and 0.87 were observed for section I, II and III indicating high levels of internal reliability. The results of the study reveal the validation of the MDQ instrument...
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Posted by admin on May 1, 2013 in |
The objective of the current study was to develop simple, precise and accurate stability indicating by isocratic RP-HPLC assay method and validated for determination of Phenobarbitone in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on a analytical C18 R column (250 mm × 4.6 mm i.d., 5 μm particle size) using mobile phase of Acetonitrile: Methanol (65:35, v/v) at a UV detector. The drug was subjected to acid degradation, alkaline degradation, oxidation, photolysis and heat to apply stress condition. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was linear in the drug concentration range of 10-60 μg/ml with a correlation coefficient 0.999. The method was also found to be robust as indicated by the % RSD values which are less than 2%. The stress degradation studies showed that Phenobarbitone undergoes degradation in acid, base, oxidation, dry heat (4.80%, 1.52%, 2.16%, and 1.81% respectively). Degradation products produced as a result of stress studies did not interfere with detection Phenobarbitone and the...
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Posted by admin on May 1, 2013 in |
Medicinal plants have been used as a source of medicine and in widespread use of herbal remedies and healthcare preparations. The present study was conducted to evaluate the antimicrobial potentiality of methanol solvent extracts of mature leaves of Costus igneus against five pathogenic bacterial isolates viz., Escherichia coli, Pseudomonas aeruginosa sp, Klebsiella pneumonia sp, Bacillus subtilis sp, Proteus mirablis sp . The antibacterial potential of Costus igneus methanol extract was tested by using Agar well diffusion method. The methanol extract of Costus igneus (100mg/ml) showed maximum zone of inhibition (7mm) against Escherichia coli sp.where as Proteus mirablis sp showed less zone of inhibition (4mm). The methanol extract of Costus igneus (100mg/ml) showed maximum zone of inhibition (7mm) against Penicillium sp. But Candida albicans sp showed less zone of inhibition (4mm). Phytochemical tests were performed and showed that the antibacterial activity of Costus igneus plant leaves was due to to the presence of phytochemical compounds like alkaloids, terpenoids, tannins, glycosides, and saponins. GC-MS analysis revealed the presence of 20...
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