Posted by admin on Jul 1, 2013 in |
The objective of the study is to design gastro retentive drug delivery systems for Amoxycillin trihydrate. It was prepared with the objective to obtain site-specific drug delivery for the stomach and to extend its duration of action. The sustained release of amoxicillin is desired because of its short biological half-life. The preparation was carried out by using guar gum as a carrier in the form of a three-layer matrix tablet. Amoxycillin trihydrate was chosen as a model drug because of its site-specificity. Matrix tablets containing either 30 % (M) of guar gum were prepared by wet granulation technique using starch paste as a binder. Three-layer matrix tablets of Amoxycillin were prepared by compressing on both sides of guar gum matrix tablet granules of Amoxycillin M with either 50 (TLM) of guar gum granules as release retardant layers. Both the matrix and three-layer matrix tablets were evaluated for weight variation, hardness, friability, buoyancy and in-vitro dissolution studies. Optimized formulation of amoxicillin was found to have increased gastric residence prolonging the...
Read More
Posted by admin on Jul 1, 2013 in |
The present work describes a validated reverse phase high performance thin layer liquid chromatographic method for simultaneous estimation of Diclofenac sodium and Famotidine in bulk and in pharmaceutical dosage form. Chromatographic separation of the drugs were performed on aluminum plates precoated with 200-μm layers of silica gel 60 RP-18 F254S as the stationary phase and the solvent system consisted of methanol: water: triethylamine (7.5:3.5:0.5) (v/v/v). Densitometric evaluation of the separated zones was performed at 291 nm. The two drugs were satisfactorily resolved with Rf values 0.54 ± 0.02 and 0.69 ± 0.02 for Diclofenac Sodium and Famotidine, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity over the range of 1500 ng/band to 9000 ng/ band for Diclofenac Sodium and over the range of 500 ng/ band to 3000 ng/ band for Famotidine...
Read More
Posted by admin on Jul 1, 2013 in |
To study the effect of herbal materials as anti-cancer agents, the first step is to evaluate their cytotoxic activity; thus in the present study, the cytotoxic activity of the extracts and fractions of Ferula szowitsiana, F. hirtella and F. oopoda in MCF-7, HT-29, A-549 and HepG-2 cells have been investigated through MTT assay. The result showed that the n-hexane and chloroform fractions of F. szowitsiana and F. hirtella were the most cytotoxic to all cell lines while F. hirtella extracts/fractions demonstrated little or no cytotoxic activity. The results suggest that the non-polar/semi polar constituents might be the cause for the cytotoxic behavior of the species....
Read More
Posted by admin on Jul 1, 2013 in |
Aims: To study effect of piracetam in experimental models of depression. Methods: Mice (n = 6/group) pretreated with distilled water, fluoxetine (28 mg/kg) and piracetam – 100, 200, 300, 400, 500, 750 and 1000 mg/kg for 7 days were subjected to tail suspension test (TST) on day 7. Rats (n=6/group) were pretreated for 7 days with distilled water, fluoxetine (20 mg/kg) and piracetam (300, 400, 500, 750 and 1000 mg/kg) and on day 7, forced swim test (FST) was conducted. Immobility time in seconds was noted in both the models and analysed using one-way ANOVA (level of significance p<0.05). Results: In TST, immobility time was reduced significantly (p<0.01) and in a dose-dependent manner with all but one dose of piracetam compared to vehicle. In FST, significant difference from control group was seen with higher doses of piracetam (500, 750 and 1000 mg/kg) with a dose-dependent trend. The mean immobility durations in the groups with significant improvement were found to be comparable to that of the respective fluoxetine groups in...
Read More
Posted by admin on Jul 1, 2013 in |
A simple, rapid, precise and accurate isocratic reversed-phase stability-indicating HPLC method was developed and validated for the simultaneous determination of Atazanavir (AT) and Ritonavir (RT) in commercial tablets. The method has shown adequate separation for AT, RT from their degradation products. Separation was achieved on a Hypersil BDS-C18, 5 μm, 125 mm × 4.6 mm i.d. column using a mobile phase consisting of buffer (pH3.4) – acetonitrile (50:50, v/v) at a flow rate of 1.5 mL/min and UV detection at 250 nm. The drugs were subjected to oxidation, acid, base hydrolysis, photolysis and heat to apply stress conditions. The linearity of the proposed method was investigated in the range of 7.8–225 μg/mL (r2 = 0.9993) for AT and 2.7-75 μg/mL (r2 = 0.9995) for RT. The limit of detection was 2.4 μg/mL for AT and 0.9 μg/mL for RT. The limit of quantitation was 7.8 μg/mL for AT and 2.7 μg/mL for RT. Degradation products produced as a result of stress studies did not interfere with the detection of AT and RT and the assay can thus be considered...
Read More
