Posted by admin on Apr 1, 2013 in |
Tannin acyl hydrolase, commonly referred to as tannase (E.C. 3.1.1.20), an inducible extra-cellular enzyme produced by a number of animals, plants and microbes. In this investigation, tannase production under solid-state fermentation by using Aspergillus niger and the waste residue of cashew husk was used as substrate for obtaining the desired fermented product. After termination of fermentation gallic acid was isolated from the tannase, and it was used in the production of an antibacterial drug Trimethoprim by using chemical and bioconversions. Microbial tannase is more stable than tannase from other sources like plants or animals. Tannase from fungal sources are reported to be active in a wide range of pH and temperature. Aspergillus niger was used in this study for the immobilization of tannase enzyme. Tannase production was reached maximum within 24 to 36 h against crude tannin extract obtained from Anacardium occidentale by using Aspergillus niger. Tannase assay was calculated. Activation energy of the immobilized enzyme was lower than that of the free enzyme. Optimum pH and temperature was...
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Posted by admin on Apr 1, 2013 in |
Purpose: The prevalence of allergic rhinitis (AR) is increasing at an alarming rate throughout the world. India has an estimated number of 15-20 million patients with allergic bronchial asthma and 30-80% of these suffer from AR. So, AR is considered as a major chronic respiratory disease due to its prevalence, impact on quality of life (QoL), work/school performance and productivity, economic burden and links with asthma. This research aims at testing the role of classical homeopathy in bringing changes in serum immunoglobulin E (IgE) level and absolute eosinophil count (primary outcome measures) and symptoms score and WHOQOL-BREF score related to AR (secondary outcome measures) by comparing the pre-trial and post-trial data. Method: An open label,single arm, experimental, prospective, non-randomized, non-controlled, before and after comparison pilot study was carried on 34 participants suffering from chronic AR. Institutional ethical clearance was obtained; then thirty four consenting patients were enrolled after screening of 58 patients by eligibility criteria and were allocated to classical homeopathic treatment. Four cases were drop-outs; thirty cases were...
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Posted by admin on Apr 1, 2013 in |
A simple, specific, precise, and efficient method for the Simultaneous estimation of Metformin HCl and Voglibose tablet by a Reverse Phase-High Performance Liquid Chromatography method is developed and validated. Selected mobile phase was in a combination of acetonitrile: buffer pH- 6.5 in the ratio of 62:38. Optimized column is a stainless steel column packed with base octa decylsilyl silica gel of 250X4.6mm and at 254 nm wavelength for metformin and voglibose detection by Spectrofluorimeter and excitation wavelength at 350nm and emission wavelength at 430nm. In our study the validation of analytical method for determination of Metformin and voglibose tablet formulation was performed in accordance the parameters including-system suitability, specificity, linearity of response, accuracy, precision (reproducibility & repeatability), robustness (change of wave length±2 nm). The method is validated according to ICH...
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Posted by admin on Apr 1, 2013 in |
In the present study, the in vitro antimicrobial and cytotoxic activity of root extract of Coccinia grandis were evaluated. Ethanolic crude extract with its various solvent soluble fractions of C. grandis showed significant antimicrobial activity by disc diffusion method on some gram positive and gram negative bacteria. The extracts exhibited 9-12 mm (approximately) of zone of inhibition comparing with standard ciprofloxacin (15-19 mm). In the brine shrimp lethality bioassay, the crude ethanolic extract showed significant cytotoxicity with LC50 of 2.496 mg/ml in comparison to vincristine sulphate (LC50 value 0.451 mg...
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Posted by admin on Apr 1, 2013 in |
Background: Self-medication is an important component of self-care. Though it is widely practiced globally, very few studies have evaluated its pattern and prevalence in dental students. Aim: The study was conducted to compare pattern of self-medication practices between junior and senior dental undergraduate students. Methods: This was a cross-sectional, anonymous, descriptive study with a six month illness recall that evaluated two groups of dental students- Group I: second year BDS students who were unexposed to pharmacology subject; Group 2: students pursuing internship (interns) having a sound knowledge of pharmacology. A questionnaire consisting of both open and close ended questions related to various aspects of self-medication was handed to the students. Consenting students anonymously filled the questionnaire. Comparison between the two groups was done by two- tailed Chi-square test. P values<0.05 were considered as statistically significant. Results: A total of 167 students participated in the study.90% respondents in group 1 (second year) and 94% respondents in group 2 (interns) practiced self-medication. Illnesses for which self-medication was practiced included fever (76%...
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