Posted by admin on May 1, 2013 in |
The ethanolic extract of barks of Caesalpinia pulcherrima (Family- Caesalpiniaceae) was evaluated for anti-diarrhoeal and analgesic activities in animal models. The crude ethanolic extract of Caesalpinia pulcherrima showed considerable anti-diarrhoeal activity on castor oil induced diarrhoea in mice and it increased mean latent period and decreased the frequency of defecation significantly (P<0.001) at the oral dose of 500 mg/kg body weight comparable to the standard drug Loperamide at the dose of 50 mg/kg of body weight. The extract of barks also produced significant (P<0.001) writhing inhibition in acetic acid induced writhing in mice at the oral dose of 500 mg/kg body weight where writhing inhibition for the extract of barks was 47.11%, which was comparable to the standard drug Diclofenac sodium at the dose of 25 mg/kg of body...
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Posted by admin on May 1, 2013 in |
Antibiotic resistance in pathogens is on the increase but in the past few decades this problem raised a deepening concern among scientific community in terms of the clinical management of infectious diseases. In this review, the common gram negative clinically significant pathogenic bacteria who have acquired resistance with passage of time like Shigella dysenteriae, Sh. Boydii, Sh. Flexneri, Listeria monocytogenes, Acinetobacter baumannii, acinetobacter, A. baumannii, Morganella morganii, Enterobacteriaceae, Citrobacter freundii, Citrobacter braakii, or Citrobacter amalonaticus, P. stuartii, Providencia rettgeri, P. alcalifaciens, P. rustigianii, E. coli, Salmonella. paratyphi, S. typhi, Salmonella enterica, have been compiled with their plausible ways of causing resistance. We conclude from our literature review that resistance in gram negative bacteria is on the rise and new and advanced antibiotics need to be designed and...
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Posted by admin on May 1, 2013 in |
Aim: To investigate the possible association of OCT1 rs122083571 polymorphism (C/T allele) with glycemic status and serum metformin levels in type II Diabetes mellitus. Methods: Sixty already diagnosed T2DM patients on metformin monotherapy (500 mg, bd) were recruited for the study and the genotypes for SLC22A1 rs122083571 C/T polymorphism using PCR assay were done. Serum fasting plasma glucose (FPG), Postprandial Plasma Glucose (PPG), Glycated Hemoglobin (HbA1c), Fasting Serum Insulin (FINS), Triacylglycerol (TG), Cholesterol (CHO), Low-Density Lipoprotein (LDL-c), High-Density Lipoprotein Cholesterol (HDL-c), and Homeostasis Model Assessment for Insulin Resistance (HOMA-IR), Body Mass Index (BMI), blood pressure (BP) were determined. Results: In patients with C allele, the BMI,BP ,FPG ,PPG and Insulin levels were significantly higher when compared to that of the T allele subjects .The values of TGL and HDL in both groups of T2DM patients were not significantly different. But the total cholesterol level and LDL level were significantly increased in T2DM patients with C allele. Conclusion: SLC22A1 rs122083571 C allele is associated with poor glycemic status in spite...
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Posted by admin on May 1, 2013 in |
Various locally available vegetables were screened for the enzyme peroxidase. The screening was performed mainly on daikon, cabbage, cauliflower; tomato and sweet potato etc. The juices of these vegetables were extracted and subjected to ammonium sulphate precipitation followed by dialysis to remove any adhere salts. The partially purified enzymes were then subjected to peroxidase assay. All the extracts from vegetables showed the presence of appreciable amount of peroxidase. In order to quantify, kinetic measurements of all the partially purified enzymes were performed using hydrogen peroxide as substrate and guaiacol (ortho-methoxy phenol) as color forming dye. The enzymatic activities of daikon, cabbage, cauliflower, sweet potato and tomato peroxidase having optical densities of 0.056, 0.139, 0.226, 0.139 and 0.089 at 280 nm were found to be 2.2898 U/ml, 3.4381U/ml, 1.1675 U/ml, 0.3741 U/ml and 0.1901 U/ml respectively. In order to compare the above result, the data have been normalized with respect to optical densities of cabbage. Thus, the partially purified enzyme having a optical density of 0.139 shows the peroxidase activity...
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Posted by admin on May 1, 2013 in |
The present study was carried out to determine the presence of phytocomponents in the aerial parts of Cleome burmanni using Gas Chromatography- Mass Spectrometry (GC-MS) analysis. The mass spectrum of the crude chloroform extract was compared with the available library sources (NIST08 LIB, WILEY8 LIB). Sixteen components were identified. Sterols, terpenoids and hydrocarbons were the most prominent. The major components detected were 24(s)- Ethyl-3- alpha, 5-alpha-cyclocholest-22 (E)-en-6-one (19.29%), Delta-4-Sitosterol-3-one (14.75%), Cholest-4-en-3-one (12.35%), Stigmasta-5, 23-Dien-3-ol, (3-beta) (12.17%), Neophytadiene (6.83%), Hexatriacontane (6.22%), 1-alanine, N-(3-fluorobenzoyl) – undec-10-enyl ester (5.96%), phytol (5.67%), tetracontane (4.30%), 1,2- benzenedicarboxylic acid (3.25%). The other components were present in minute...
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