Posted by admin on May 1, 2013 in |
The objective of the current study was to develop simple, precise and accurate stability indicating by isocratic RP-HPLC assay method and validated for determination of Phenobarbitone in solid pharmaceutical dosage forms. Isocratic RP-HPLC separation was achieved on a analytical C18 R column (250 mm × 4.6 mm i.d., 5 μm particle size) using mobile phase of Acetonitrile: Methanol (65:35, v/v) at a UV detector. The drug was subjected to acid degradation, alkaline degradation, oxidation, photolysis and heat to apply stress condition. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was linear in the drug concentration range of 10-60 μg/ml with a correlation coefficient 0.999. The method was also found to be robust as indicated by the % RSD values which are less than 2%. The stress degradation studies showed that Phenobarbitone undergoes degradation in acid, base, oxidation, dry heat (4.80%, 1.52%, 2.16%, and 1.81% respectively). Degradation products produced as a result of stress studies did not interfere with detection Phenobarbitone and the...
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Posted by admin on May 1, 2013 in |
Medicinal plants have been used as a source of medicine and in widespread use of herbal remedies and healthcare preparations. The present study was conducted to evaluate the antimicrobial potentiality of methanol solvent extracts of mature leaves of Costus igneus against five pathogenic bacterial isolates viz., Escherichia coli, Pseudomonas aeruginosa sp, Klebsiella pneumonia sp, Bacillus subtilis sp, Proteus mirablis sp . The antibacterial potential of Costus igneus methanol extract was tested by using Agar well diffusion method. The methanol extract of Costus igneus (100mg/ml) showed maximum zone of inhibition (7mm) against Escherichia coli sp.where as Proteus mirablis sp showed less zone of inhibition (4mm). The methanol extract of Costus igneus (100mg/ml) showed maximum zone of inhibition (7mm) against Penicillium sp. But Candida albicans sp showed less zone of inhibition (4mm). Phytochemical tests were performed and showed that the antibacterial activity of Costus igneus plant leaves was due to to the presence of phytochemical compounds like alkaloids, terpenoids, tannins, glycosides, and saponins. GC-MS analysis revealed the presence of 20...
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Posted by admin on May 1, 2013 in |
Self-medication is global problem but in developing regions, where drugs are available to customers easily it arises greater risks and challenges. Peshawar and Mardan are two big and heavily inhibited cities of a developing country, Pakistan hence in this study we aim to find the level of self-medication and general knowledge about antibiotics of the people in these two cities. We conducted our study on 500 participants from these two cities (250 from each) through a simple questionnaire. Our results confirmed that 78% of participants were using self-medication we also report that 45.2% of the participants prefer self-medication to a physician prescription. Our results also showed that 25.6% of the participants never check the expiry date on the antibiotics they use during self-medication. We also found that 63.6% of the participants believed that they know what type of antibiotic to take for which medical complications however in contrast 34.4% of participant reported that antibiotics could be used in dengue fever. We conclude that the knowledge and perception of inhabitants...
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Posted by admin on May 1, 2013 in |
Calliandra portoricensis a medicinal plant, is widely use in the treatment/management of various diseases in Nigeria. The ethyl acetate extract of the Calliandra portoricensis root bark were tested to evaluate the cytotoxicity, antioxidant (in vitro) and free radical scavenging activity. The different assay methods, including total antioxidant activity, free radical (nitric oxide, hydroxyl radicals and lipid peroxidation) scavenging assays were used to evaluate the antioxidant potential of the ethylacetate extract. While cytotoxic activities were evaluated using brine shrimps lethality assays. The methanol extract showed total antioxidant capacity of 2.362µg/mg of plant extract expressed as ascorbic acid equivalents (AAE) compared to 1µg/mg ascorbic acid; the extract exhibited two-fold scavenging activity with IC50 of 364.175 and 313.52µg/ml for nitric oxide and hydroxyl radicals compared to IC50 of 231.31µg/ml and 228.78µg/ml of ascorbic acid as standard respectively. The extract conferred 50% protection at the concentration of 51.92μg/ml on lipid peroxidation induced by FeSO4 in liver mitochondria. The Brine shrimp lethality bioassay of the extract showed cytotoxic activity of (LC50 = 0μg/ml) which...
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Posted by admin on May 1, 2013 in |
Plant derived drug molecules are frequently showing their role in treating the disease conditions with minimal side effects comparing to the synthetic molecules. Forest and trees are natural assets that provide a variety of benefits to the mankind. Currently available anthelmintic drugs require improved management, high cost and also worms developing resistance to these drugs. So, there is a need of investigation of new anthelmintic molecules. Blumea densiflora (compositae) and Salvinia cucullata (salviniaceae) are selected as test drugs for evaluation of anthelmintic activity. Helminth infections are distressing huge population in the world. These infections are contributing to the disorders like pneumonia, anaemia, eosinophilia and under nourishment. Indian earthworm, Pheretima posthuma was used for evaluating the anthelmintic activity due to its anatomical and physiological resemblance with the intestinal roundworm parasites of human beings. All the extracts shows significant anthelmintic activity compared to the standards. They exhibited varying degree of activity in a dose dependent manner and causes paralysis of the worms followed by death. The aqueous extract of Salvinia cucullata...
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