Posted by admin on Feb 1, 2013 in |
The objective of the current study was to develop and validate simple and precise UV Spectrophotometric method for estimation of Diclofenac Sodium in the swab samples to validate cleaning procedure. The swabbing procedure was optimized in order to obtain a suitable recovery from stainless steel surface using Tex wipe polyurethane swab stick. Detection wavelength selected was 267 nm. The proposed method was validated in terms of Linearity, precision, accuracy, limit of detection and limit of quantitation. Linearity was studied over concentration range of 0.5 -3 μg / ml and correlation coefficient was found to be 0.999 for regression line. A recovery obtained was 94.58 %. LOD and LOQ for developed method were found to be 0.014715 µg/ml and 0.044592 µg/ml...
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Posted by admin on Feb 1, 2013 in |
The present study was aimed at investigating the chemical composition and antifungal activity of Ethanol Extracted Haramaya propolis (EEHP). The GC-MS analysis of EEHP showed the presence of sixteen compounds and twelve compounds were identified by means of their retention times, by comparison of their mass spectra with the NIST 2005 library data and literature. The major constituents of EEHP were Benzenamine, N, N-dibutyl- (21.94%), Paromomycin (9.74%), 4-Aminobutyramide, N-methyl-N-[4-(1-pyrrolidinyl)-2-butynyl]- (9.26%) and DL-Tryptophan, 5-methoxy (7.43%). The Crude EEHP showed antifungal activities against Fusarium sp., Aspergillus niger and Colletotrichum sp. GC-MS results showed that the antifungal activities of Haramaya propolis could be because of the presence of 3-(α-Hydroxyethyl)-aniline, Benzenamine,N, N-dibutyl, DL-Tryptophan,5-methoxy, 6-Amino-5, 8-dimethoxyquinazoline, Paromomycin and Imidazole,...
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Posted by admin on Feb 1, 2013 in |
A study was undertaken to evaluate the efficacy of five various commercial products of ciprofloxacin (a broad spectrum antibiotic), manufactured by different pharmaceutical companies. The drug was evaluated for their performance based on equivalent MIC breakpoint, which is a measure of efficacy of drugs against Staphylococcus aureus ATCC6538 and Pseuomanas aeruginosa ATCC25668 when tested in vitro.The data showed variation for all manufacturers with significant difference (p <0.0001) in efficacy of these drugs at the final dilution level (0.02-0.1µg/µl) against P.aeruginosa. Thus the ciprofloxacin, prepared by various manufacturers are not equally efficacious in inhibiting the growth of S.aureus and P.aeruginosa. Therefore, manufacturers should pay special attention for stringent quality control of drug, and clinicians are suggested to use these antibiotics judiciously in the treatment of patients for these...
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Posted by admin on Feb 1, 2013 in |
Gastro retentive controlled release drug delivery system of Verapamil HCl was formulated to increase the gastric retention time of the dosage form with controlling the drug release pattern. Different grades of hydroxy propyl methyl cellulose derivatives; Methocel K4M and Methocel K15MCR were incorporated for their gel forming properties. Tablet buoyancy was achieved by incorporating a mixture of gas generating agents; sodium bicarbonate and anhydrous citric acid. In vitro dissolution studies were carried out for eight hours using USP XXII paddle type apparatus using 0.1N HCl as the dissolution medium. All the gastro retentive tablets showed good in vitro buoyancy. Tablets swelled radially and axially during buoyancy study. The release kinetics were explored and explained with zero order, first order, Higuchi and Korsmeyer equations. The release rate, extent and mechanisms were found to be governed by polymer type and content. Formulations were characterized by physical characterization, drug loading content and Fourier Transform Infrared spectroscopy (FT-IR). Good results were obtained in the tests and the FT-IR spectroscopic studies indicating no interaction...
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Posted by admin on Feb 1, 2013 in |
The present work aims at evaluating the anti-inflammatory activity of Samadera indica by HRBC membrane stabilization. The prevention of hypotonicity induced HRBC membrane lysis was taken as a measure of the anti-inflammatory activity. The anti-inflammatory activity of the crude ethanol extract, aqueous extract of leaves part of Samadera indica were compared to that of the standard drug diclofenac. The extract in concentration of 250-1000 µg/ml showed a dose dependent inhibition of haemolysis of erythrocyte induced by hypotonic solution. The ethanolic extract of Samadera indica showed significant anti-inflammatory activity in comparison to aqueous extract and with standard drug...
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