Posted by admin on Dec 1, 2012 in |
The main aim of our study is to evaluate the antiurolithiatic activity of pomegranate (punica granatum, Family: Puniaceae) and garlic (Allium sativum, Family: Liliaceae). For this, we have subjected the seeds of pomegranate and garlic bulbs to maceration using 95% of ethanol, ethyl acetate, petroleum ether and water at a ratio of 1:4 for 24 hours, followed by reflux over a water bath for 3 hours and the obtained extract was concentrated under vacuum. The prepared extracts were then subjected to titrimetry and colorimetry for the estimation of calcium and phosphate respectively. By comparing the result of different extracts, we have found that ethanol extract of garlic has showed better result in inhibiting precipitation of calcium and phosphate. But individual isolation and further studies are to be carried out to confirm this...
Read More
Posted by admin on Dec 1, 2012 in |
The objective of the present study was to investigate the antioxidant and analgesic activity of ethanol extract of Xanthium indicum Koen. Family: Compositae. Ethanol extract of X. indicum plant was prepared and tested for its phytochemical and pharmacological investigation. Preliminary phytochemical investigation showed that extracts possessed the active principles-alkaloid, carbohydrate, glycoside, flavonoid & tannin. The ethanol extract of X. indicum showed significant antioxidant property in the qualitative assay. The extract showed the antioxidant property by the presence of strong yellow spot on a purple background on the TLC plate & it also in the quantitative assay, X. indicum extract displayed free radical scavenging activity in the DPPH assay (IC50 ~141.25 µg/mL) which is comparable to standard (ascorbic acid, IC50 ~14.12 µg/mL). The extract showed dose-dependent reduction ability (Fe3+ to Fe2+ transformation) in reducing power assay; showing a maximum absorbance of 0.492 at a concentration of 100 μg/mL of the ethanol extract comparable to that of ascorbic acid which were used as positive control and gave maximum absorbance of 0.659...
Read More
Posted by admin on Dec 1, 2012 in |
Nosocomial infections even in this modern era antibiotics, continue to remain an important and formidable consequence of hospitalization. Our aim of the study was to investigate the antimicrobial susceptibility against hospital-associated infecting microorganisms. 30 Clinical samples were taken from OPD of GMC Hospital, Bhopal (MP), India. Among 30 clinical isolates we identified bacterial pathogens as Staphylococcus aureus (10), Escherichia coli (10) and Pseudomonas aeruginosa (8). Antimicrobial susceptibility assay was performed by the reference criteria of clinical and laboratory standard institute guidelines. In the present study antibiotic susceptibility results showed that all 10 (100%) S. aureus isolates were resistant to penicillin, 8 (80%) S. aureus isolates were resistant to cefuroxime, 7 (70%) S .aureus isolates were resistant to ciprofloxacin, 5 (50%) S. aureus isolates were resistant to amikacin, 2 (20%) S. aureus isolates were resistant to nitrofurantoin whereas, 0 (0%) S. aureus isolates were found to be resistant to cefazolin. For E. coli isolates all 10 (100%) were resistant to cefuroxime, 7 (70%) E. coli isolates were resistant to amikacin,...
Read More
Posted by admin on Dec 1, 2012 in |
This research was targeted to reveal the phytochemical activity of Casuarina equisetifolia against susceptible pathogenic oral bacteria. Antibacterial effect of the plant was examined by well diffusion method. Different plant parts were screened for the phytochemicals using various polar solvents like ethanol, acetone, methanol, chloroform and water. Studies disclosed that Casuarina equisetifolia encompasses a wide range of phytochemicals with anticariogenic activity. During phytochemical analysis by thin layer chromatography four compounds were obtained in the acetone solvent system with highest Rƒ value of...
Read More
Posted by admin on Dec 1, 2012 in |
Enzyme engineering is a fast-growing application in the pharmaceutical market. Enzymes are key to new processes because they are environmentally friendly and reduce hazardous waste. The uses of immobilized enzyme eliminate the enzyme separation step from the main process thus simplifying and increasing the overall process yield. The use of immobilized enzyme has several advantages as compared with an application of free enzymes. Immobilized enzymes can be recovered from reaction mixture and can be made available for reuse again. This work was undertaken to study the rate and duration of digestion of Bovine Serum Albumin by pepsin in free enzyme and immobilized states. Immobilization of pepsin was done by entrapment in calcium chloride. After studying the observations, it is found that in the reaction catalyzed by free pepsin, after a certain period of time; there was no more increase in optical density even if the enzyme concentration was increased. This indicates that the total liberation of amino acids related with increased absorbance gets in limiting stage due to exhaustion...
Read More
