Posted by admin on Feb 1, 2013 in |
A High Performance Liquid Chromatographic method was developed for determination of Diclofenac Potassium in in-vivo study during bioavailability study. The chromatographic separation was conducted on Shimadzu Prominence LC 20 connected with PDA detector; using mixed column ODS/Cyano; ACE, (100 x 4.6 mm, 5 µm). The mobile phase was isocratic consisted of Methanol: 50 mM potassium dihydrogen phosphate buffer, in ratio of (50 : 50 v/v) and was delivered to the system at a flow rate of 1.5 ml/min, with an injection volume of 20 µl and the detection wavelength (λ max) was 280 nm. Diazepam was used as internal standard. All assays were performed at ambient conditions. The calibration curve of Diclofenac Potassium in plasma was linear with correlation coefficient (r2) = 0.9996; over a concentration range of 0.25 – 3 ug/ml. The retention times for Diclofenac Potassium and the internal standard (Diazepam) were found to be 5.95 and 7.90 minutes, respectively. The mean recovery was found to be 90.57%.The relative standard deviation (RSD) was found to be <...
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Posted by admin on Jan 1, 2013 in |
Dalbergia spinosa Roxb. (Leguminosae) is a mangrove plant native to Asia and enjoys number of traditional uses. This paper describes the investigation of antibacterial and cytotoxic activity of the ethanol extract of D. spinosa leaves. Antibacterial activity of the ethanol extract of D. spinosa leaves was tested by disc diffusion assay and broth macrodilution assay. The extract was also tested for brine shrimp lethality. The extract showed antibacterial activity against a wide range of Gram-positive and Gram-negative bacteria in both assays. The minimum inhibitory concentrations (MIC) against these bacteria were in the range of 250-500 μg/mL. The LC50 against brine shrimp nauplii was 84.14 μg/mL. The above data demonstrates that the plant has some important biological activities with sufficient potency and further studies can be performed to identify the active...
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Posted by admin on Jan 1, 2013 in |
Felodipine is a long-acting 1, 4-dihydropyridine calcium channel blocker, used to control hypertension by selective action on peripheral resistance. The conventional felodipine tablet gives a rather high peak and comparatively low trough levels, due to rapid absorption and distribution. More sustained plasma concentrations might thus produce a more even effect on blood pressure. The main aim of the study was to improve dissolution rate of the dosage form in a controlled manner over extended period of 24 hrs. Matrix tablets were prepared by direct compression method,using hydrophobic polymers like Glyceryl monostearate and Carnauba wax. The prepared formulations were evaluated for hardness, thickness, weight variation, friability and in-vitro dissolution studies. Among all the formulations F8 was selected as optimized formulation based on the evaluation parameters and in-vitro release profile of 100% drug release for 24 hrs. The FTIR and DSC results of optimized formulation showed no drug-excipient interaction. For optimized formulation(F8), the drug release mechanism was explored and explained by zero-order (r2=0.984), first-order (r2=0.947), Higuchi (r2=0.967) and Korsmayer-peppas (r2=0.982 &...
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Posted by admin on Jan 1, 2013 in |
The present time is an era of revolution as rapid changes and developments have been noticed in almost all spheres around the globe. In most of the countries the private sector has played a pioneer role to introduce several changes. The pharmaceutical industry through research and development has brought out a variety of products. There is much of experimentation, researches and trials. The improved technology in pharmaceutical industry requires a wide variety of essential manufacturing equipments and material. In the process of manufacturing, since a wide range of activities are involved this has an adverse impact on the environment. Thus it brings along matters of concern. With the changing time, the era of modernization is coming up with many more problems. To cope with such problems, the production of drugs is also being carried out on a massive scale. The procedures involved in these activities and manufacturing of drugs causes several, perhaps unnoticeable, environmental tribulations. So, the pharmaceutical companies should take it as a social and moral responsibility to...
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Posted by admin on Jan 1, 2013 in |
The main objective of this study was to prepare controlled release matrix type ocular inserts of mizolastine for the treatment of seasonal allergic conjunctivitis.The films were prepared by solvent casting technique using Eudragit RL100 and RS100 in different ratios with dibutylphthalate as the plasticizer. The films were evaluated for the physicochemical parameters. In-vitro studies were carried out using Franz-diffusion cell (bi-chamber compartment model)and ex-vivo studies of the optimized formulation were carried out using goat’s cornea. In vivo studies were performed using rabbit as the animal model. Formulations F4andF6, which showed controlled and prolonged in vitro drug release, were subjected to in vivo study. In vitro and in vivo correlation was found to be good, revealing the efficacy of the formulations.Formulation F6 was found to be promising, as it achieved the objective of the present...
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