Posted by admin on Nov 1, 2012 in |
Punica granatum L. belongs to the family Punicaceae, which is originating from the Middle East, extending throughout the Mediterranean, eastward to China and India. Punica granatum has been used extensively as a traditional medicine in many countries for the treatment of dysentery, diarrhea, helminthiasis, acidosis, hemorrhage and respiratory pathologies. In addition, P. granatum is reported to have antioxidant, anti-atherosclerotic and antiviral properties. The dried pomegranate peels (20gms) were extracted using water (100ml) & ethanol (100 ml). The Ethanolic Extract of Punica granatum (EPG) was active against the tested bacteria. EPG demonstrated promising antibacterial activity against tested gram positive and gram negative bacteria. The E. coli and S. aureus shows more susceptibility to...
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Posted by admin on Nov 1, 2012 in |
A new series of 2, 5-disubstituted-1, 3, 4-oxadiazole derivatives were synthesized using appropriate methods & structures of the synthesized compounds were confirmed by IR,NMR & Mass spectral data.. These ligands were prefiltered for their drug likeness properties by Lipinski’s rule of 5 and it was found that all the ligands satisfied the rule of 5 for oral bioavailability.The prefiltered ligands were subjected for docking studies using integrated web server called docking server to investigate the interactions between the target compounds and the amino acid residues of the Glycogen synthase kinase-3β. The docking studies were done using auto dock between computationally designed 2,5-disubstituted 1, 3, 4-oxadiazole derivatives and Glycogen synthase kinase-3beta (GSK-3β) protein. The Calculated free energy of binding and estimated inhibition constants (Ki) were remarkable when compared with the standard GSK-3 inhibitors. It is calculated by the Lamarckian Genetic Algorithm (LGA). These values & the proposed interactions suggested that the 2, 5-disubstituted-1, 3, 4-oxadiazole derivatives are excellent inhibitors of...
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Posted by admin on Nov 1, 2012 in |
Some new series of 3-(4-substituted-phenyl)- 2-thioxo-2,3-dihydroquinazolin-4(1H)-one, 3-(4-substituted-phenyl)-2-(methylthio)quinazolin-4(3H)-one and 3-(4-substituted-phenyl)-2-hydrazinylquinazolin-4(3H)-one were synthesized by cyclization of 4-substituted-phenylcarbamodithioate with methyl anthranilate followed by reaction with dimethylsulphate and then prepared compound was refluxed with hydrazinehydrate respectively. The starting material 4-substituted-phenylcarbamodithioate was synthesized from 4-substituted-aniline. The title compounds were investigated for antibacterial and antifungal activity by disc diffusion technique. Compound IIa, IIb and IIIa showed moderate activity against B. subtilis, E. coli and A. niger as compared to standard drug. Compound IIIb and Iva exhibited good activity against A. niger. Compound IIIc and IVc was found to be most active compound against E. coli and moderately active against B. subtilis and A. niger, of the prepared...
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Posted by admin on Nov 1, 2012 in |
An accurate, sensitive and precise RP-HPLC –Fluorescence method has been developed and validated for the estimation of Carvedilol Phosphate (CP) from bulk drug and Pharmaceutical Dosage form. The separation was achieved by a Brownlee analytical C18 column (250mm X 4.6mm, 5μm) in isocratic mode, with mobile phase comprises of Acetonitrile : Methanol : Buffer in proportion of 70:20:10v/v/v, buffer was 5mM Potassium Di-hydrogen Phosphate (pH 3.5 adjusted with Ortho Phosphoric Acid). The flow rate of mobile phase was 1.0ml/min and employing fluorescence detection with 280nm excitation and 340nm emission wavelengths. The retention time of Carvedilol Phosphate was 2.20 min.The calibration curve was found to be linear within the concentration range of 10ng/ml to 60ng/ml. The regression data for calibration curve shows good linear relationship with r2 = 0.990. The method was validated in accordance with the requirements of ICH guidelines. Moreover, the proposed analytical method was applied to monitor the formulation commercially...
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Posted by admin on Nov 1, 2012 in |
The present study was designed to evaluate anti-hyperglycemic activity of simvastatin alone and the combination of sub therapeutic doses of simvastatin and glipizide. Hyperglycemia was induced experimentally in albino rats by subcutaneous injection of alloxan in a dose of 175 mg/kg body weight. After 72 hours of alloxan treatment, rats showing hyperglycemia (blood glucose level of 400 mg/dl and above) were included in the study. They were divided into four groups of 6 animals each (n=24). Oral administration of normal saline 0.5 ml, glipizide 2.5 mg/kg body weight, simvastatin 10 mg/kg body weight and sub therapeutic doses of both test (simvastatin 5 mg/kg body weight) and standard (glipizide 1.25 mg/kg body weight) drugs, was done respectively into each of the four groups for 30 consecutive days in order to assess the effect in terms of reduction in blood glucose level. Blood glucose was estimated on 0th, 10th, 20th, and 30th days of study in fixed time intervals. In the test group, there was a gradual fall in the blood...
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