Posted by admin on May 31, 2023 in |
This study aimed at identifying promising breast cancer inhibitors through in-vitro and in-silico studies. Ocimum basilicum. L is a traditional herb used for varied ailments. The present study is designed to evaluate the anti-carcinogenic potency of Palustric acid and Andrographolide against the MCF-7 cell line by in-vitro analysis. Further in-silico examination was performed to detect and formulate Receptor-ligand complex of estrogen using molecular docking technique. In-vitro study was conducted using MTT assay and microscopic examinations to determine the cell viability and morphological changes in MCF-7 cells. In-silico, scrutiny was performed using virtual screening, Docking, ADME, and molecular dynamic simulation to evaluate the stability of the compounds. Palustric acid and Andrographolide against the MCF-7 cell line by in-vitro analysis. Further in-silico examination was performed and showed an outstanding anti-cancer potential, with dose- and time-dependent patterns in MTT assay and through the fluctuating organization of MCF-7 cells. In-silico analysis showed that the selected lead compound-complex exhibited good stability and was a highly potent inhibitor against the target breast cancer receptor. This...
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Posted by admin on May 31, 2023 in |
In the present study, biosurfactant-producing microorganisms such as KM1 (Bacillus licheniformis – Kasemedu 1), KM2 (Bacillus subtilis – Kasemedu 2), ER1 (Pseudomonas aeruginosa – Ennore site 1), ER1B (Pseudomonas mendocina – Ennore site 1) and ER9 (Streptococcus lactis – Ennore site 2) were subjected to optimization studies. The optimization study was carried out using various carbon, nitrogen sources, pH, temperature, hydrocarbons, and trace elements. The effect of dry cell biomass, biosurfactant production, emulsification activity, and surface tension were studied. Among the five isolates, KM2 and ER1 grew well in sucrose at 1% concentration. Production and activity of the biosurfactant were found to a maximum at pH 7.0, and an additional increase in pH during bacterial growth does not affect the surfactant activity till pH 8.5±0.2; however, a decrease in pH showed a significant effect on the surfactant activity. The optimum temperature was identified as 37°C for all the isolates and below and above this temperature markedly affects the biosurfactant production and activity. Observations on reduction in surfactant activity with...
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Posted by admin on May 31, 2023 in |
The relevance of traditional medicines and their folkloric applications for the well-being of humanity have been highlighted in the current study. To generate the polyherbal extract for the in-vitro assessment of anticancer activity, the researcher has investigated the standardization criteria for the individual plant drug and extract obtained from these herbs. According to the literature and earlier studies, all four of the herbs utilized to create polyherbal extract have the potential to cure a variety of malignancies in addition to liver carcinoma. The physiochemical and pharmacological value of each medicinal herb and the polyherbal extract of these four plant drugs (Vinca, Jackfruit, Lawsonia, and Ashwagandha) are revealed in the same research findings. Based on the results of phytochemical testing, it is evident that the majority of these phytochemicals employed include the alkaloids and steroids in n-hexane extract, which also suggests the possible anticancer effects of each drug separately. The MTT dye assay assessed the polyherbal extract’s cytotoxic impact on the Huh7 cell line. The polyherbal extract’s anticancer results are...
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Posted by admin on May 31, 2023 in |
The present investigation aimed to provide an approach for the solubility and bioavailability enhancement by Comparative solubility of cyclodextrins with Lornoxicam (LXM) by forming ternary Inclusion complexation (TIC). The phase-solubility and saturation solubility studies were executed in the existence of β-CD, HP-βCD and polymers. The drug/CD complexes were formulated and characterized further to confirm complex formation using DSC and FTIR. The solid dispersions (SDs) were formulated using double hydrophilization and accessed using FT-IR, DSC, and in-vitro testing. The equilibrium phase solubility and saturation solubility study for both binary and TIC showed a more significant solubility enhancement of LXM-HP β-CD- poloxamer-407(PXM 407) than LXM-β-CD. The FT-IR and DSC analysis confirmed the formation of a TIC amongst LXM-HP-βCD-PXM 407. The TIC demonstrated a higher Complexation Efficiency (CE) and stability constant than binary. The drug dissolution rate (DR) profile showed significant drug release from developed TIC and improved DR to a greater extent. From comprehensive study findings, it can be stated that employing the TIC of LXM-HP β-CD with PXM 407 might...
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Posted by admin on May 31, 2023 in |
Aegle marmelos (L) Correa also known as Bale, belongs to family Rutaceae. The plant is used to treat several diseases like bronchitis, asthma, malarial, astringent, laxatives, digestive and febrifuge. Leaves are used as antipyretic, antimicrobial, antidiabetic, antihistaminic, anti-inflammatory, antioxidant, and hyperlipidaemic activity. The plant contains diterpenoids, triterpenoids, and phenolic compounds. The present investigation was carried out to isolate, purify, and characterize active constituents from the Aegle marmelos (L) Correa plant. Powdered material of the whole Aegle marmelos (L) Correa plant was taken to prepare extract using Soxhlet apparatus. Various phytochemical tests were performed for alkaloids, glycosides, phenolic, flavonoids, etc. TLC was done for the isolation of compounds. GC-MS, thin layer chromatography, and Nuclear magnetic resonance further characterized the isolated compounds. The anti-asthmatic was evaluated by using in-vitro models. The Petroleum ether extract of Aegle marmelos (L) Correa significantly inhibited histamine contraction and confirmed the significant anti-asthmatic...
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