Posted by admin on Sep 1, 2012 in |
Aim of present investigation was to isolate natural mucoadhesive agent from Ocimum americanum seeds and characterize through in vitro mucoadhesion methods, FTIR and DSC studies. Mucoadhesion force of isolated natural mucoadhesive agents and synthetic polymer Carbopol 934P was determined using in vitro mucoadhesion methods viz. Wihelmy’s method, falling sphere method and modified physical balance method. The research study reveals that the natural mucoadhesive agent isolated from Ocimum americanum seed was shown promising mucoadhesion strength. The formation of hydrogen bond by natural mucoadhesive agent with mucosa was confirmed by FTIR spectra showing carboxyl and hydroxyl groups. Natural mucoadhesive agent may be useful to formulate mucoadhesive drug delivery systems as it bears excellent mucoadhesion property and advantageous over synthetic polymers for less toxicity, biocompatibility, biodegradability and...
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Posted by admin on Sep 1, 2012 in |
Paracetamol is a widely used non-prescription analgesic and antipyretic medicine. The study was conducted to assess the comparative in-vitro quality control parameters through the evaluation of weight variation, hardness, friability, disintegration time and dissolution profile between the commercially available tablet brands of paracetamol. Tablets of two manufacturers of the formulation were evaluated in two groups A and B. The similarities were found between both the groups. Both tablet brands of paracetamol (1.0 to 1.6%) showed acceptable weight variation and friability (below 1%). Both tablet brands were somewhat different in their hardness, disintegration time and dissolution profile. It can be concluded that standard quality control parameters always should be maintained not for paracetamol but also for all kinds of medicine for getting better drug...
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Posted by admin on Sep 1, 2012 in |
To analyse the situation of antibiotic resistance, a total of 77 E. coli isolates from urine, pus, sputum and endo-tracheal aspirate were screened for their antibiograms for antibiotic resistance, multiple antibiotic resistance (MAR) index for evaluating the spread of resistance and plasmid profiles for the presence and characterization of plasmids. Very high resistance level (>90%) was detected against ampicillin, amoxicillin, ceftazidime, norfloxacin, tetracycline while imipenem and amikacin recorded the least resistance levels of 2.3% and 13.9% respectively among the isolates. An increased resistance to amoxicillin, tetracycline, cotrimoxazole and norfloxacin were observed in this geographical area which however displayed a lower resistance in other countries. The MAR index varied considerably, the lowest was 0.18 and the highest was 0.89. Plasmids of 10 size ranges were detected in the isolates. Some isolates possessed single sized plasmid while other possessed multiple plasmids. Isolates with high multi- antibiotic resistance profiles were found to possess multiple plasmids. This study shows that regular antimicrobial sensitivity surveillance is necessary and acquisition of plasmid could greatly contribute...
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Posted by admin on Sep 1, 2012 in |
The methanol extract of Centella asiatica (Family:Umbelliferae) was studied for its phytochemical and pharmacological Activities. This study of the extracts indicates the presence ofReducing Sugar Tannins, Alkaloids, Sapponins and.The pharmacological study include Antimicrobial activities.The extract of the plant showed activity against a wide a variety of microorganism’s .All the activities were compared by measuring the zone of inhibition with the standard antibiotic. Based on the findings of Antimicrobial activity, we can say that the plant may contain novel compounds that possess potent anti-mutagenic activity and anti-oxidative activity. The obtained results support for the uses of this plant as traditional medicine. Infectious diseases are the second leading cause of death worldwide. Treatment of infections continues to be problematic in modern time because of the severe side effects of some drugs and the growing resistance to antimicrobial agents. The activities observed could be attributed to the presence of some of the phytochemicals detected which have been associated with antibacterial...
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Posted by admin on Sep 1, 2012 in |
A Reversed Phase Ultra Performance Liquid Chromatographic (RP-UPLC) method was developed for the determination of glipizide (GLI) in in-vitro study during formulation development. The chromatographic separation was achieved on a Acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 µm) column, with a mixture of 55 volumes of methanol and 45 volumes of buffer contain 0.001M sodium dihydrogen phosphate in isocratic elution with flow rate of 0.3 mL/min. The eluted compound was monitored at a wavelength of 276 nm using a UV detector. The method described herein separated glipizide and glipizide related compound-A from all other formulation components within a run time of 1.2 min. The RP-HPLC method was developed and validated according to the International Conference on Harmonization (ICH) guidelines. This method was successfully applied in the quantitative determination of glipizide in in-vitro study during formulation development. The procedure described herein is simple, selective, and reliable for routine quality control analysis as well as stability...
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