Posted by admin on Jul 1, 2012 in |
Averrhoa bilimbi Linn. (Family: Oxalidiaceae) is a medicinal plant which is extensively used in traditional medicine to cure cough, cold, itches, boils, rheumatism, syphilis, diabetes, whooping cough, and hypertension. In this study, hydromethanolic extract of A. bilimbi fruits was examined for its antioxidant action using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and total antioxidant capacity. Total phenol and flavonoid contents of the extract were also estimated. Moreover, brine shrimp lethality bioassay was used to investigate the cytotoxic potential of A. bilimbi. The extract exhibited strong DPPH radical scavenging activity with and IC50 value of 20.35μg/ml as opposed to the IC50 value of the reference standard, ascorbic acid (12.6μg/ml). It also displayed remarkable total antioxidant capacity (417.093±6.577mg/g in ascorbic acid equivalent (AAE)). Total phenol and total flavonoid contents of the extract were 106.16±2.818 mg/g in gallic acid equivalent (GAE) and 276.73±25.25 mg/g in quercetin equivalent (QE), respectively. A. bilimbi fruit extract also showed strong cytotoxic potential with an LC50 value of 5.011μg/ml in brine shrimp lethality bioassay. Our results suggest that,...
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Posted by admin on Jul 1, 2012 in |
Indian Gooseberry, Emblica officinalis is a, fruit widely accepted in Kerala for its medicinal & nutritive values. The fruit has dark green colored spherical seed surrounded by pale green colored tissue. Different varieties of food products, pickles being the major one, are used all over the world .The present work on gooseberry originated from a real medical case history. The medical reports say that a normal person who was eating gooseberry on a normal course all at a sudden became allergic only to this fruit since two years. This person was hospitalized two times for anti- dot injection as the allergic symptoms go beyond control. The major symptoms the patient showed are; (a) itching; (b) swelling of the whole bodily parts & (c) drowsiness. These allergic effects were due to gooseberry and which confirmed by medical examination. Based on our preliminary information, allergy due to Indian gooseberry was the first case reported in our locality. This made our interest to investigate further the chemistry & physiology of allergic reactions...
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Posted by admin on Jul 1, 2012 in |
Lornoxicam, a widely prescribed anti-inflammatory and analgesic drug belongs to class II under BCS and exhibit low and variable oral bioavailability due to its poor aqueous solubility. It is practically insoluble in water and aqueous fluids. As such its oral absorption is dissolution rate limited and it requires enhancement in solubility and dissolution rate for increasing its oral bioavailability. The objective of the present study is to enhance the solubility and dissolution rate of lornoxicam employing Hydroxy propyl β-cyclodextrin (HPβCD) and two surfactants (SLS and Tween 80) alone and in combination. The individual main effects and combined (interaction) effects of HPβ-cyclodextrin and surfactants on the solubility and dissolution rate of lornoxicam were evaluated in a series of 22 factorial experiments. The solubility of lornoxicam in the four selected fluids as per 22 factorial study in each case was determined (n=4). Lornoxicam-βCD-surfactant inclusion complexes were prepared employing the selected combinations of HPβCD and surfactant in each case as per a 22 factorial design and the inclusion complexes prepared were evaluated...
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Posted by admin on Jul 1, 2012 in |
Schiff base ligand (E)-4-[(hydroxyl phenylimino)methyl]benzene-1, 2-diol (H3L) was prepared from the condensation reaction of Protochatechu aldehyde (3, 4-dihydroxybenzaldhyde) with 2-amino phenol. From the direct reaction of the ligand (H3L) with Co(II), Ni(II) and Cu(II) chlorides and Fe(III) and Zn(II) nitrates in 2M:1L molar ratio, the five new neutral complexes were prepared. The characterization of the newly formed compounds was done by 1HNMR, UV-Vis and IR spectroscopy and elemental analysis. The in-vitro antibacterial activity and antifungal activity of the metal complexes were studied and compared with that of free ligand. The Cupper complex shows the higher biological...
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Posted by admin on Jul 1, 2012 in |
Mitragyna speciosa Korth. is a member of the Rubiaceae family. More than 25 alkaloids have been isolated from Mitragyna speciosa. Mitragynine is the major alkaloid of this plant and is responsible for antinociceptive action. No single study is available about the effect of mitragynine on aminopyrine N-demethylase activity in rat hepatocytes. Experiments were undertaken to evaluate the effect of mitragynine in different age groups (adult & old) of Sprague- Dawley (SD) male and female rat hepatocytes. In vitro this evaluation was assessed by different concentration of mitragynine (0.0025µM-250µM).Hepatocytes were prepared by collagenase perfusion technique. Aminopyrine N-demethylase activity was determined by measuring the quantity of formaldehyde formed. Results showed that a significant increase in aminopyrine N-demethylase activity was observed in the adult male, female and old female SD rat hepatocytes treated with 250µM mitragynine (p< 0.05). However, the old male rat did not show any significant change at any concentration of mitragynine. In conclusion this study indicates the induction of hepatic drug metabolizing enzymes by mitragynine is affected by the...
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