Posted by admin on Jul 1, 2012 in |
The use of medicinal plants and preparations derived from them as dietary supplements, nutraceuticals, functional foods and herbal medicinal products has become more widely accepted in developing countries. Therefore, it is important to evaluate the adverse effects of these plants and their preparations. The objective of this study was to evaluate the safety of the methanol extracts Plumeria acuminata (MEPA) and Galega purpurea (MEGP) by single the long-term oral administration in mice. The study of chronic toxicity was determined by oral feeding male mice daily with the MEPA and MEGP at the dose of 300, 600 and 1,200 mg/kg body weight continuously for 270 days. The results of chronic toxicity showed no abnormalities in the test groups as compared to the controls. Hematological and blood chemical values in treated groups were normal in comparison with the control group. Non-toxicity effect of MEPA and MEGP were present as no changes in body weight, internal organ weight, and general behaviors. Macroscopic or microscopic of internal organs or tissues in treated rats...
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Posted by admin on Jul 1, 2012 in |
The present research work was aimed at the enhancement of solubility of Candesartan by Self Emulsifying Drug Delivery Systems (SEDDS). Candesartan is a BCS class II drug having low aqueous solubility and high permeability; hence its bioavailability is solubility rate limited. The saturated solubility of Candesartan in various oils and surfactants was determined. The excipients were screened and selected showing maximum solubility and compatibility for Candesartan. SEDDS formulations of Candesartan were developed using different Oils, Surfactants and Co-Surfactant combinations. Pseudoternary phase diagrams were constructed using Triplot V 4.1.2 software and applying Pseudoternary phase diagrams, microemulsification area was evaluated. Formulations were prepared based on phase diagrams using various proportions of oil, surfactants and co-surfactants. The formulations were screened visually for stability and phase separation. Seven formulations were selected for further evaluations like effect of dilution, freeze-thawing, emulsion droplet size and zeta potential. Among the seven formulations three were optimized and filled in hard gelatin capsules. The in-vitro dissolution studies of the SEDDS formulation were performed and the dissolution rate of...
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Posted by admin on Jul 1, 2012 in |
A normal phase high performance thin layer chromatography (HPTLC) method has been developed and validated for simultaneous quantitative determination of two flavonoids viz. rutin and isoquercitrin from dried leaf powder of Jasminum sambac Ait. The analysis was performed on TLC aluminum plates precoated with silica gel 60F254. Linear ascending development was carried out in a twin-trough glass chamber saturated with mobile phase comprised of ethyl acetate: methanol: formic acid (10.0:2.0:1.0 v/v/v) at room temperature 25±2ºC. The densitometric scanning was done at λ=366nm for both the components in reflectance/absorbance mode. The detector response was linear for concentrations ranging from 0.20µg/band to 0.65µg/band and 0.30µg/band to 1.2µg/band for rutin and isoquercitrin respectively with correlation coefficient of 0.999 for both the components. The method was precise as the value of percent relative standard deviation was found to be less than 2. The amounts of rutin and isoquercitrin in the dried leaf powder of Jasminum sambac Ait. were found to be 0.4962mg/g and 0.6461mg/g respectively. The accuracy of the developed HPTLC method was...
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Posted by admin on Jul 1, 2012 in |
Cell wall polysaccharides have been of great importance to society and mankind over the years. In the present study Cell wall polysaccharides were extracted from different stages viz- Immature green, mature green and ripe stages of Prunus armaniaca Linn. A decrease in the yield of pectin was observed during ripening.The pectin Extracted from various stages of fruit ripening was further analysed for in vitro antioxidant activity by different assays. During the ripening of fruit it was seen than the pectin extracted showed increase in total antioxidant, reducing power and scavenging of hydrogen peroxide which can be related to galacturonic acid content by further studies and can be explored as a novel potent...
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Posted by admin on Jul 1, 2012 in |
The leaves of Paederia foetida Linn. (P. foetida) are commonly known as skunk vine or Chinese fever vine, are used for various ailments medicinally throughout Asia and other tropical parts of the world by traditional healers. The plant is mainly used for arthritis and rheumatic disorders. The whole plant shows tonic, astringent and antiphlogistic actions and has been used in tenesmus. This present work presents a detailed pharmacognostical study of the leaf of the crude drug P. foetida. The samples were studied using procedures of light, confocal microscopy, WHO recommended physico-chemical determinations and authentic phytochemical procedures. The physico-chemical, morphological and histological parameters presented in this study may be proposed as parameters to establish the authenticity of P. foetida and may possibly help to differentiate the drug from its...
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