Posted by admin on Apr 1, 2012 in |
Amylases are among the most important enzymes in present-day biotechnology. The effects of various carbon and nitrogen sources on α-amylase production by submerged fermentation (smF) from Bacillus amyloliquefaciens was investigated. The study revealed that the levels of the α- amylase production varied greatly with the type and concentration of carbon and nitrogen sources used. The highest α-amylase production was obtained in medium supplemented with lactose at 5g/l concentration. The other carbon sources like sucrose and mannitol also favored the α-amylase production. Maximum enzyme production was found with Ammonium chloride (NH4Cl) as the nitrogen source at 6g/l concentration. Yeast extract and peptone also seems to be suitable for the production of α-amylase from Bacillus...
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Posted by admin on Apr 1, 2012 in |
A simple, precise and stability-indicating ultra-performance liquid chromatography (UPLC) method for simultaneous determination of Paracetamol (PCM) and Lornoxicam (LOR) in tablet formulations has been developed and validated. The UPLC separation was achieved on a Waters Acquity UPLC BEH shield RPC18 (2.1x50mm, 1.7µm) column using acetonitrile:30mM Ammonium acetate (40:50, v/v) as mobile phase at a flow rate of 0.5mL/min. The eluted compounds were monitored at 260nm, the run time was within 5.0min, which PCM and LOR and its impurities were well separated. Quantitation was achieved over the wide concentration range 0.5-150μg/mL and 10-48μg/mL with mean recovery of 100.97 ± 0.31% and 99.73 ± 0.71% for PCM and LOR, respectively. Linear regression analysis revealed linearity in the range of 0.5-150µg/band and 10-48µg/band for PCM and LOR respectively. Drugs were subjected to the stress conditions of acid, alkali hydrolysis, oxidation for degradation. PCM was found to stable in base, acid, alkali, oxidative stress conditions and LOR was found to degraded significantly in base, and stable in acid/alkali, oxidative degradation conditions. The degradation...
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Posted by admin on Apr 1, 2012 in |
Metal nanoparticles have been using as an ingredients in the preparation of complementary medicines to cure different diseases is an age old medicinal practices. The plant based Ayurvedic preparations are preferred by 80% of the world population and WHO is encouraging the green medicine due to its less side effects. Hence an attempt has been made to screen this important medicinal plant for secondary metabolites biogenesis of silver nanoparticles (SNPs) and their antimicrobial efficacy. The stem, roots, stembark and leaves were selected for synthesis of SNPs. The SNPs formation was confirmed with the help of UV-VIS spectroscopy and characterized by AFM. The phytosynthesized SNPs were tested for antibacterial and antifungal activities using paper disc diffusion method. The results indicate that the selected plant revealed that it is source for different secondary metabolites like anthocyanins, emodins, triterpenoids, tannins, saponins, luecoanthocyanins, reducing sugars, flavonoids, lignins, anthroquniones, phenols and glycosides. But steroids, coumarins, fatty acids and alkaloids are absent. The SNPs are having more anti microbial efficacy than that of the plant...
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Posted by admin on Apr 1, 2012 in |
Kalanchoe pinnata (Crassulaceae) commonly known as Jakh me hayat. Kalanchoe pinnata pers is present naturalized throughout the hot and moist parts of India, particularly common in Bengal. The plant is antibacterial, Anticancer, Antiparasitic, Anti-insecticidal, Anti-allergic. Phytochemical studies had revealed the presence of alkaloids, flavonoids, triterpenes, glycosides, steroids and lipids. Present study was carried out to determine, the morphological, microscopical and phytochemical profiles. Microscopy of stem show endodermis cortex, collateral types of vascular bundle and lignified xylem vessels, etc. The physical parameter such as ash value and extractive value were evaluated. The anti-inflammatory effect of 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4h H chromen-4-one isolated from the medicinal herb Kalanchoe pinnata pers stem, was evaluated for its anti-inflammatory activity in the Swiss albino-induced rat paw oedema models of acute inflammation. Flavonoid at doses of 200 and 400 mg/kg, the clinical anti-inflammatory indomethacin at 10 mg/kg, or vehicle were administered orally before injection of the pro-inflammatory compound. The test compound showed significant anti-inflammatory activity against paw edema induced by carrageenin, most notably at the highest test dose...
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Posted by admin on Apr 1, 2012 in |
The antibacterial efficacy of the crude methanolic and aqueous extracts of Annona senegalensis, Pers on clinical isolates of E. coli, Salmonella enteriditis and Shigella dysenteriae were determined using Agar Well Diffusion technique on Mueller Hinton Agar (MHA) plates. At the concentration of between 100 and 200mg/ml, the methanolic extract inhibited the growth of all the organisms with an average zone of inhibition diameter ranging from 30±0.2 to 35±0.36mm for E. coli and S. Enteriditis while for S. dysenteriae, it ranged from 21±0.22 to 26±0.36mm. On the other hand, the aqueous extract did not exhibit activity on any of the test organisms. The Minimum Inhibitory Concentration (MIC) of the methanolic extract on the organisms ranged from 0.39mg/ml on E. coli, 3.17mg/ml on S. enteriditis 25.00mg/ml on S. dysenteriae. The result from this study suggests that the crude extract from the stem bark of A. senegalensis could be used in the treatment of gastroenteritis caused by these...
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