Posted by admin on Jun 1, 2012 in |
The present manuscript describes simple, sensitive, rapid, accurate, precise and economical spectrophotometric method for the simultaneous determination of prednisolone acetate and ofloxacin in combined eye drop dosage form. The method is based on the simultaneous equations method for analysis of both the drugs using methanol as solvent. Prednisolone acetate has absorbance maxima at 243 nm and ofloxacin has absorbance maxima at 228 nm in methanol. The linearity was obtained in the concentration range of 5-17 μg/ ml and 1-13 μg/ml for prednisolone acetate and ofloxacin respectively. The concentrations of the drugs were determined by using simultaneous equations method at both the wavelength set 243nm for PRD and 228nm for OFL. The method was successfully applied to pharmaceutical dosage form because no interference from the eye-drop excipients was found. The suitability of this method for the quantitative determination of prednisolone acetate and ofloxacin was proved by validation. The proposed method was found to be simple and sensitive for the routine quality control application of prednisolone acetate and ofloxacin in pharmaceutical...
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Posted by admin on Jun 1, 2012 in |
A simple, rapid, sensitive and cost effective difference spectrophotometric method have been developed for the determination of Febuxostat in its tablet dosage form. The method is based on the induced spectral changes upon changing the pH of the medium that differ in their UV spectra. Difference spectrum, obtained by keeping Febuxostat in 0.1N NaOH in reference cell and same in 0.1N HCl in sample cell, showed two characteristic peaks at 260nm and 315nm with positive and negative absorbance respectively and viceversa. Difference of absorbance between these two maxima was calculated to find out the amplitude, which was plotted against concentration. The calibration curve is linear over the concentration range of 5-25 µg/ml (r2 = 0.999), with a detection limit of 0.58µg/ml. The method was successfully applied to the commercial pharmaceutical drug without interference from common ingredient accompanying the drug along with its dissolution profile and very cost effective as its prevents the use of hazardous and costly organic...
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Posted by admin on Jun 1, 2012 in |
A new series of flurobenzothiazole incorporated 1, 3, 4 – thiadiazole compounds have been synthesized. The structure of the synthesized compounds was confirmed by UV, IR, 1H NMR, Mass spectral analysis and evaluated for their antimicrobial activity against Proteus vulgaris NCTC 4635, Micrococcus leutus NL98, Aspergillus flavus ATCC 46646by disc diffusion method. The compounds SH8 and SH11 were also evaluated for the anti-inflammatory activity by carrageenan-induced paw oedema method. The synthesized compounds (SH6 to SH11) showed good antimicrobial activity. However the antimicrobial activity of the synthesized compounds against the tested organisms was found to be less than that of respective standard drugs used at tested dose level. The anti-inflammatory activity confirmed that the test compound SH11 showed superior activity in the inhibition of oedema than SH8. However, both the test compounds were found to be less active than the standard drug...
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Posted by admin on Jun 1, 2012 in |
A new, simple, sensitive and reproducible spectrophotometric method has been developed for the estimation of Flucloxacillin in pure form. Method involves the determination of Flucloxacillin by dissolving in water and followed by measuring the absorbance at 273 nm. The linearity was obtained in the concentration range of 100-300 µg /ml. The suitability of method for quantitative analysis of Flucloxacillin was proved by validation. This method was extended to tablet formulation and there was no interference from excipients and diluents. This method has been statistically validated and is found to be precise and...
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Posted by admin on Jun 1, 2012 in |
Solubility is one of the important parameter to achieve desired concentration of drug in systemic circulation for pharmacological response to be shown. Poorly water soluble drugs often require high doses in order to reach therapeutic plasma concentrations after oral administration. Any drug to be absorbed must be present in the form of an aqueous solution at the site of absorption. Most of the drugs are weakly acidic and weakly basic with poor aqueous solubility. Hence various techniques are used for the improvement of the solubility of poorly water-soluble drugs include micronization, chemical modification, pH adjustment, solid dispersion, complexation, co‐solvency, micellar solubilization, hydrotropy etc. The purpose of this work was to describe the enhance solubility of rifampicin by using solid dispersion technique and Physical mixture with PEG6000. Here drug and carrier ratio 1:1, 1:2, 1:3 and 1:10 respectively. The prepared samples were evaluated by SEM, Drug content, In-vitro studies, Wettability and Solubility, IR studies, Angle of repose. In-vitro drug release showed fast and complete release over a period of 2hrs...
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