Posted by admin on May 1, 2012 in |
In recent days, it has been given great emphasis on the scientific evaluation of traditional or complementary and alternative medicines in order to justify the traditional use, safety, efficacy, quality, and to know its therapeutic values that have not yet explored. Chandanasav, an ancient Ayurved formulation, is commonly used for the treatment of malnutrition, presence of semen in the urine, and treatment of heart and autoimmune diseases. Till now, no research information is available on the immuno-stimulatory activities of this preparation. The present study was designed to evaluate the preliminary immunostimulating potential of Chandanasav for polyclonal immunoglobulin M (IgM) production and proliferation of murine spleen cells. BALB/c mice splenocytes were treated with 0, 0.25, 0.5, 0.75, 1, 1.5, 2, 3 and 4% (v/v) of Chandanasav and the cells were sub-cultured at 37oC in a humidified atmosphere containing 5% CO2 for 120 h. IgM production in cultured supernatant was determined by Enzyme-Linked Immunosorbent Assay (ELISA) and splenocytes proliferation was measured by 3-(4, 5-dimethyl thiazol-2-y)-2, 5-diphenylterazolium bromide (MTT) assay. Chandanasav at...
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Posted by admin on May 1, 2012 in |
The antioxidant activity of methanol extract of whole plant of Synedrella nodiflora as well as its petroleum ether, carbon tetrachloride, dichloro-methane and aqueous soluble partitionates were evaluated by DPPH of (1, 1-diphenyl-2-picrylhydrazyl) and phosphomolybdenum total antioxidant assay and compared with standard antioxidants butylated hydroxytoluene (BHT) and ascorbic acid (ASA). The total phenolic content was also determined and expressed in gallic acid equivalent (mg of GAE/g of sample). A great variance was observed for polyphenol content as well as antioxidant activity (1.574-9.4136 mg GAE/g and DPPH IC50 10.52-31.25 μg/ml) depending on the nature of solvent used to fractionate the crude extract. The result demonstrated that dichloromethane soluble fraction revealed the highest amount of phenolic compounds (9.4136 mg GAE/g) and also had significant free radical scavenging activity (IC50 10.52 μg/ml). A positive correlation was observed between total phenolic content and total antioxidant activity of S. nodiflora having correlation coefficient (R2) of 0.9270. The general toxicity of the extractive was studied by brine shrimp lethality bioassay and from the results (LC50 0.023-0.122...
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Posted by admin on May 1, 2012 in |
In Ethiopian traditional medicine, the aqueous decoction of the leaves of Cineraria abyssinica Sch. Bip. exA. Rich (Asteraceae) is used for treatments of various ailments including diarrhea, however, to date, there appear to have been no reports on the phytochemistry and the antimicrobial activity of the plant. The main aim of this study was, therefore, to carry out preliminary phytochemical screening and antimicrobial activities on leaf extracts of C. abyssinica. The in vitro antimicrobial activities of the aqueous and 80% methanolic crude extracts of the leaves of C. abyssinica were investigated against Gram positive and Gram negative bacteria by agar disc diffusion method. Both the aqueous and 80% methanolic extracts showed various degrees of potent antibacterial activities comparable to the standard drug ciprofloxacin against all of the bacteria tested except Bacillus species. Preliminary phytochemical screening of the plant revealed the presence of polyphenols, flavonoids, coumarins, saponins, terpenoids and phytosterols. The acute toxicity studies showed the nontoxic nature of the plant up to 3 g/kg. Therefore, the present study revealed...
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Posted by admin on May 1, 2012 in |
The curative parts of medicinal plants are not simply its woody stem or its leaves but the number of chemical compounds (phytoconstituents) it produces and uses for its own growth like flavonoids, alkaloids, gum, mucilage, protein, carbohydrate etc. The genus Phyllanthus is widely used in medicine but still lot of work left to explore this genus. So the species, Phyllanthus emblica which is widely used in Indian traditional system are selected for various screening. The mentioned plant materials are rich source of polyphenolic compounds and some typical type of polyphenolic are responsible for various pharmacological activities. The isolation and characterization of Phytoconstituents was done from the alcoholic extract by the chromatographic method. The structure of the isolated compound was established on the basis of physical, chemical test and spectroscopic evidences (IR, UV, 1HNMR, MS). A flavonoidal structure was isolated from the alcoholic extract of the plant. The yellow colored compound which solubilizes in methanol, ethanol and water having m.p. 315oC and Rf value 0.19 in mobile phase Isopropanol: formic...
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Posted by admin on May 1, 2012 in |
Two chemical compounds have been isolated and their structures were elucidated by spectroscopic studies including VLC, TLC and NMR. The isolated compounds are Ortho-hydroxy-N- benzyl- 16- Methyl- 11, 14- diene-octadecamide (1) and 9, 12-diene–octadecanoic acid...
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