Posted by admin on Mar 1, 2012 in |
Comparative study of free radical scavenging activity and phenolic acid content of Bulgarian endemic Alchemilla jumrukczalica and the commercial herbal – Alchemilla vulgaris complex was carried.The methanol extracts of studied samples showed significant antiradical activity. The concentration of the extracts needed for 50% inhibition of DPPH was found to be 12,09 µg/ml and 19,62 µg/ml for A. jumrukczalica and A. vulgaris complex, respectively.Ten free and seventeen bonded phenolic acids were identified and quantified by performed of gas chromatography mass-spectrometry (GC-MS). Gentisic, protocatechuic, salicylicand trans-cinnamic acids were represented in the greatest quantityamong the identified phenolic acids. This is the first detailed analysis on qualitative and quantitative composition of phenolic acids inAlchemilla...
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Posted by admin on Mar 1, 2012 in |
A simple, precise and stability-indicating HPLC method was developed and validated for the simultaneous determination of anti-hypertensive drugs Atorvastatin Calcium, Olmesartan Medoxomil, Candesartan, diuretics Hydrochlorothiazide and Chlorthalidone. The separation was achieved on Cosmosil PAQ (Length 150 mm × Diameter 4.6 mm Particle size 5 μm) column with gradient flow. The mobile phase at a flow rate of 1.0 mL min−1 consisted of 0.05 M sodium dihydrogen phosphate buffer and acetonitrile (Gradient ratio). The UV detection was carried out at 220 nm. The method was successfully validated in accordance to ICH guidelines. Further, the validated method was applied for commercially available pharmaceutical dosage...
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Posted by admin on Mar 1, 2012 in |
The present study was carried out to illustrate the relative evaluation of phytochemical constituents and antimicrobial activity of leaves of Eclipta alba and Aphanamixis polystachya and bark of Premna integrifolia in two different solvents. A preliminary phytochemical analysis was done and concluded that the presence of alkaloids, tannins, flavonoids and glycoside in both extracts of the above plants. The antimicrobial activity was tested using disc diffusion method against eight pathogenic bacteria using Kanamycin as antibiotic standards. The outcome showed that the antibacterial activity was effective in chloroform and ethyl acetate fractions of tested extracts. The MIC was determined in two different solvents of both extracts and observed that chloroform and ethyl acetate possess minimum inhibition in both extracts. From the current investigation, it can be concluded that Eclipta alba, Aphanamixis polystachya and Premna integrifolia crudeextracts have quite effective antimicrobial activity and potent phytochemical constituents....
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Posted by admin on Mar 1, 2012 in |
Efavirenz, a widely prescribed anti retroviral drug belongs to class IΙ under BCS and exhibit low and variable oral bioavailability due to its poor aqueous solubility. Its oral absorption is dissolution rate limited and it requires enhancement in the solubility and dissolution rate for increasing its oral bioavailability. The objective of the study is to evaluate the feasibility of formulating efavirenz –βCD– Poloxamer 407 /PVP K30 inclusion complexes into tablets and to evaluate the effects of βCD, Poloxamer 407 and PVP K30 on the dissolution rate and dissolution efficiency of efavirenz tablets in 23 factorial study. A comparative evaluation of wet granulation and direct compression methods was made for the preparation of tablets employing drug – βCD – Poloxamer 407 / PVP K30 inclusion complexes. Drug – βCD- Poloxamer 407 / PVP K30 inclusion complexes were prepared by kneading method. Tablets each containing 100 mg of efavirenz were prepared by wet granulation and direct compression methods employing various βCD complexes as per 23 factorial design and the tablets were...
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Posted by admin on Mar 1, 2012 in |
This study was subjected to investigate in vitro anti-inflammatory, antioxidant and cytotoxic properties of methanol extracts of two Bangladeshi bean pods namely Lablab purpureus L. sweet ‘white’ and ‘purple’. Protease Inhibition method was used to determine in-vitro anti-inflammatory properties. DPPH free radical scavenging method, total flavonoids, Total antioxidants and reducing power of the two samples were assessed for antioxidant properties and cytotoxicity potentials were assessed by the brine shrimp lethality test.In in-vitro anti-inflammatory investigation there is a linear relation of %inhibition for the white bean pods which indicates having positive anti-inflammatory property. In DPPH test the lowest and highest IC50 values are 430.00µg/ml and 853.13μg/ml, with L. purpureus sweet ‘purple’ and L. purpureus sweet ‘white‘ respectively and compared with standard ascorbic acid. In Cytotoxicity test LC50 value was found 960.06 μg/ml for L. purpureus (L.) sweet ‘purple’ and 66.5 µg/ml for L. purpureus sweet ‘white’, So L. purpureus sweet ‘white’ is more potent. The total Flavonoid contents of the test samples are 42.55±5.77 and 32.09±0.36 mg/g quercetin equivalents for...
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