Posted by admin on Jul 31, 2023 in |
Background: An emerging respiratory disease was abbreviated as COVID 19, after it has been first reported in December 2019 in Wuhan city of China. The major route of transmission of COVID-19 is droplet and close contact. Government of India has initiated training for health care workers at a different level. WHO and CDC also initiated a multidisciplinary approach to tackle COVID-19 of which awareness creation is the main. Thus, the main objective of this study is to assess knowledge, attitude, and practices of medical undergraduates within periphery of Gujarat, India towards COVID-19. Methods: In this cross-sectional study, 505 medical students were surveyed online using developed, validated questionnaire during two week period. A p-value of <0.05 indicated statistical significance. Results: Among 505 students, 59% (n= 298) are males and 41% (n=207) are females. Medical students have 85 % very good knowledge towards covid-19 which is done during early phase of lockdown (25th march 2020 to 19th May 2020). About 81.6% positive attitude observed in study participants. We discovered that about...
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Posted by admin on Jul 31, 2023 in |
In this present study, a simple, effective, and accurate method has been developed for estimating itraconazole and terbinafine in bulk and pharmaceutical dosage form using UV Visible spectrophotometer. Two methods were developed: the simultaneous equation method, also known as vierordt’s method, and the other absorbance ratio method, also known as Q value method. In the simultaneous equation method, maximum absorption was found at 229 nm for itraconazole and at 223 nm for terbinafine. In the absorbance ratio method, two different wavelengths were selected, 229 is being the lmax of one drug and 225nm is another iso-bestic wavelength. The linearity was found in the range of 5 -25µg/ml for both drugs and methods, with the correlation coefficient (R2) of itraconazole and terbinafine is 0.999 for simultaneous equation method. For the absorbance ratio method, itraconazole’s correlation coefficient (R2) is 0.9953 and 0.9877, and terbinafine is 0.9903 and 0.9948. All validation parameters were studied after method development, including linearity, accuracy, precision, limit of detection, limit of quantitation, robustness, and ruggedness as per...
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Posted by admin on Jul 31, 2023 in |
Nowadays, polyherbal formulations are in demand for managing and treating many diseases owing to their less side effects. Thus, qualitative and quantitative standardization of these herbal formulations as per WHO and ICH guidelines is essential to ensure the quality and efficacy of the formulations. The present study involves the development of a simple and precise UV/Visible spectrophotometric method for simultaneous estimation of curcumin and ascorbic acid in the polyherbal antidiabetic formulation at their respective λmax 422 nm and 266 nm. The method obeys Beer’s law in the concentration range of 2 to 10 μg/mL for both curcumin and ascorbic acid with a correlation coefficient of 0.9992 and 0.999, respectively. The limits of detection (LOD) and quantitation (LOQ) were found to be 0.225 μg/mL and 0.675 μg/mL for curcumin and 0.12 μg/mL and 0.36 μg/mL for ascorbic acid, respectively. The obtained results demonstrated that the proposed method is simple, sensitive, and cost-effective and can conveniently be employed for the routine analysis of any polyherbal formulations containing curcumin (Curcuma) and ascorbic...
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Posted by admin on Jul 31, 2023 in |
Cutaneous aspergillosis is a rare locally invasive disease in immunocompromised patients caused primarily by Aspergillus species which induce primary infections through injured skin, burns, or locally infect via fungal contaminated adhesive dressings or intravenous catheter insertion, resulting in lesions to erythematous papules. About 80% of aspergillosis is caused by the ubiquitous and fastest-growing airborne fungi Aspergillus fumigatus leads to mortality in immunocompromised patients. Thioredoxin systems are one of the two main antioxidant systems in maintaining their cellular redox homeostasis essential for cell viability and occurring in all life forms. Thioredoxin reductase is one of the components of Thioredoxin system responsible for the normal growth of Aspergillus fumigatus. The present study deals with the in-silico analysis of binding efficiencies between phytochemicals from different medicinal plants with the target protein thioredoxin reductase of Aspergillus fumigatus using bioinformatics tools. The phytochemicals and target protein structure were retrieved from PubChem and PDB (ID:6BPY) databases, respectively. Qikprop module was used for ADME profiling of phytochemicals and the selected phytochemicals were subsequently docked with protein...
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Posted by admin on Jul 31, 2023 in |
A series of novel thiazolidine-4-one-pyrazole hybrids (4a-4j) had been synthesized by Claisen-Schmidt condensation, Schiff’s base, and Cyclization mechanism. The newly synthesized target molecule structures were confirmed by IR, 1H NMR and Mass spectral data. The synthesized hybrids were screened for in-vitro anticancer and anthelmintic activities. The anticancer activity was assessed using MTT assay against MCF7 and SKVO3 cell lines and Doxorubicin as the reference standard. Anthelmintic activity of compounds was studied by using Albendazole as standard. Most synthesized hybrids (4j, 4h, 4b) showed good anticancer and anthelmintic activities. The molecular docking studies were performed using the Ligprep tool of the Schrodinger suite. This study revealed that novel thiazolidne-4-one-pyrazole hybrids (4a-4j) had good interaction with the active site of EGFR receptor. The compound 4j reported the highest dock score of -4.229 with a Glide binding energy of -52.98 Kcal/mol. The pyrazole hybrids’ dock score ranged from -4.229 to -2.19...
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