Posted by admin on Apr 30, 2023 in |
PCOS (Polycystic ovarian syndrome) is a prevalent female endocrine disorder with global occurrence ranging from 6-26%. It accounts for the majority of anovulatory infertility in women of reproductive age due to enlarged ovaries with small cysts formation, which the National Institute of Health has reported as a cause of 30% of infertility cases in females worldwide. Anovulatory infertility is generally treated with ovulation induction therapy using drugs to promote and facilitate ovulation. However, these medications’ varying success rates and numerous adverse effects compel the search for more promising and safer alternatives. In this research study, 10 ovulation induction agents i.e., Tamoxifen, Metformin, Clomiphene, Pioglitazone, Letrozole, Rosiglitazone, Anastrozole, Prednisone, Dexamethasone, and Spironolactone, were selected to analyze their pharmacokinetics, drug-likeness, bioactivity profile, and toxicity profile through the in-silico approach. The results present information about each drug, including its pharmacological competency and level of toxicity. This study may aid in the direction of future research and the development of more effective ovulation-inducing medications....
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Posted by admin on Apr 30, 2023 in |
Objective: This dissertation work aimed to prepare Levocetirizine HCL (LCH) nasal in-situ gel, a second-generation antihistamine, to improve its retention time, sustain release, enhance the bioavailability, and avoid first-pass metabolism. Process: The in-situ nasal gel was prepared to consume Carbopol as a gelling agent, Hypromellose (HPMC) as a thickening or thickness-imparting agent, and buffering agents to maintain pH. An assessment was conducted to determine the solubility, pH, rheological characteristics, consistency of content, adhesive properties to mucous membranes, gel strength, in-vitro release characteristics, the kinetics of release date, isotonicity, and sterility of the sample. Results: All formulations had a clear appearance with good gelling capacity, and drug content was found to be >87%. The pH values of the formulations were found to be acceptable, ranging from 6.2 ± 0.10 to 6.4 ± 0.44, and no irritation of the mucosal membranes was observed. The shearing character was found between 200-1400 at pH 6.4 and 2000-5500 cps at pH 7.4 when measured at 20 rpm. They also showed adequate gel strength of...
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Posted by admin on Apr 30, 2023 in |
Optimizing the cultural conditions to enhance bioactive metabolite production by Streptomyces rubrus VLK-24 isolated from mangrove sediments as well as characterizing these compounds, has been taken up in this study. The culture broth inoculated in Yeast extract, Malt extract, and Dextrose broth and extracted with ethyl acetate exhibited high antimicrobial activity against test microorganisms, including Gram-negative and Gram-positive bacteria as well as fungi. The crude ethyl acetate extract exhibiting high antimicrobial activity was analyzed by Gas Chromatography-Mass Spectroscopy, which evidenced the presence of 39 compounds according to the available library data, NIST MS Search (ver. 2.0). The results of the study revealed the production of diversified metabolites by the strain; hence it could be a possible source for novel antimicrobial...
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Posted by admin on Apr 30, 2023 in |
For the present study, 10 bacterial strains were isolated from agriculture field soils collected from Guntur, Andhra Pradesh, India, and thereafter screened for amylase activity. Among them, the strain DS5 showed a maximum zone of clearance on the starch hydrolysis test was selected for this study. Identification of bacterial strain DS5 was confirmed by 16S rRNA sequencing analysis. The phylogenetic tree constructed on the basis of 16S rRNA sequences revealed that it clustered with the closest members of Bacillus pumilus DS5 and the sequences were deposited in NCBI Gen bank along with accession number MK430437. Further the effect of incubation period, temperature, pH, carbon and nitrogen sources was optimized. Highest amylase production was found after 48 h of incubation at pH 7.0 and 40 ⁰C temperature. Starch proved to be the best carbon source and peptone as nitrogen source for the highest production of amylase by Bacillus pumilus DS5. Supplementation with different nitrogen and carbon source favoured the increased enzyme yield by submerged...
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Posted by admin on Apr 30, 2023 in |
Diabetes mellitus is a complex disorder where oxidative stress plays a crucial role in the development and progression of the disease. In the present work, the rat model evaluated pancreas and liver tissue damage caused by a high-fat diet and diabetic complications. Male Wistar rats were administered with HFD- corn oil orally for 28 days followed by streptozotocin (STZ) injection to induce diabetes on 29th day. The drug treatment groups were given low dose (250mg/kg) and high dose (500mg/kg) of hydroalcoholic extract of Achyranthes aspera (HEAA), and its therapeutic efficacy in HFD + diabetes model was compared with the standard drug metformin. Various antioxidant parameters were measured, including MDA, protein, GSH levels, SOD, and CAT activities. The histology of pancreas tissues was also analyzed using Haematoxylin, Eosin, and Masson-trichrome staining. The expression of insulin protein in the pancreas was studied by immunohistochemical technique. The effect of drugs on GLUT-2 and PPAR-α gene expression showed considerable improvement in the liver tissues of different groups. From the results, it can be...
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