Posted by admin on Jul 1, 2011 in |
Cassava ((Manihot esculenta Crantz) leaf stalk is a renewable raw material and it is a major industrial crop in Tamilnadu rich in antioxidant compounds. To utilize this source, an environmentally sustainable procedure has been developed for the extraction of anthocyanin and to analyze its antioxidant properties. So, the present research was focused on cassava for total phenol contents, flavanoids, anthocyanins and it’s antioxidant activities were studied using leaf stalks. Three difficult solvents were tried to extract total phenol and anthocyanin from cassava leaf stalks. In the case of total phenol content acetone extract found to have maximum phenol content followed with acidified methanol and methanol. In the case of anthocyanin content, acidified methanol extract gave maximum yield followed with methanol and acetone extracts. The presence of phenolic compound and anthocyanin determine the free radical scavenging activity in cassava leaf stalk extract. The cassava leaf stalks, which was found to be a waste material after harvesting the tubers can be used as a valuable source for anthocyanin extraction, which will...
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Posted by admin on Jul 1, 2011 in |
Reactive oxygen species (ROS) are thought to underline the process of ageing and the pathogenicity of various diseases, such as neurodegenerative disorders and cancer. The use of traditional medicine is widespread and plants still present a large source of natural antioxidants that might serve as leads for the development of novel drugs. In this study, an ethanolic extract of leaves from Dillenia indica L. which belongs to family “Dilleniaceae”, was tested for in vitro antioxidant activity. Free radical scavenging assays, such as hydroxyl radical, hydrogen peroxide, 2, 2-diphenyl-1-picryl hydrazyl (DPPH), and reducing power assay were studied. The extract effectively scavenged hydroxyl, hydrogen peroxide radicals. It also scavenged DPPH radicals. Furthermore, it was found to have reducing power. All concentrations of leaf extract exhibited free radical scavenging and antioxidant power, and the preventive effects were in a dose-dependent manner. The results obtained in the present study indicate that the ethanolic extract of Dillenia indica L. could be considered a potential source of natural antioxidant....
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Posted by admin on Jul 1, 2011 in |
Purpose: The present study was undertaken to investigate diuretic effect of aqueous and methanol extracts of the dried seeds of erythrina indica in normal rats. Method: Aqueous and methanol extracts of erythrina indica seeds were administered to experimental rats orally at doses of 25 and 30 ml/kg p.o. Furosemide (10 mg/kg) was used as positive control in the study. The diuretic effect of the extracts was evaluated by measuring urine volume, sodium and potassium content, conductivity and chloride content. Result: Urine volume was significantly increased by the two doses of aqueous and methanol extracts from root and bark of erythrina indica in comparison to control group. While the excretion of sodium was also increased by both extracts, potassium excretion was only increased by the aqueous extract at a dose of. 25 ml/kg. There was no significant change in the conductivity and pH of urine after administration of the erythrina indica extracts. The diuretic effect of the extracts was comparable to that of the reference standard (Furosemide). Conclusion: We can...
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Posted by admin on Jul 1, 2011 in |
A simple, precise, rapid and accurate Reverse Phase HPLC method was developed for the estimation of Teicoplanin in Parenteral dosage form. An Xterra RP C18, 250×4.6 mm, column with 5 µm particle size and the mobile phase consisting of 0.02M Potassium Dihydrogen Orthophosphate + 0.02M Dipotassium Hydrogen Orthophosphate + 0.5% Triethyl Amine in water pH: 3.2 adjusted with Orthophosphoric Acid: Acetonitrile (40:50). Acetonitrile in the isocratic mode was used. The flow rate was 0.5 ml/min and the effluents were monitored at 210 nm. The retention time was 4.178 min. The detector response was linear in the concentration of 24.1-289.2 mcg/mL for Teicoplanin, the respective linear regression equation being Y (-242937.9235) = 71026.949x + 174544.8363 for Teicoplanin. The Limit of Detection (LOD) and The Limit of Quantification (LOQ) were 1.205 mcg/mL and 3.615 mcg/mL respectively for Teicoplanin. The percentage assay of Teicoplanin was 99.64 %. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple,...
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Posted by admin on Jul 1, 2011 in |
Phytochemical constituents present in the aqueous and ethanolic extract of Syzygium cumini seeds were investigated which were found to be responsible for its medicinal efficacy. The major phytoconstituents present in S. cumini seeds were further determined by GCMS analysis which showed the presence of polyphenols, sesquiterpenes, n-alkanes. The antibacterial activity of ethanolic extract of S. cumini seeds were tested against common human pathogens by agar well diffusion method and minimum inhibitory concentration required to inhibit the growth of various pathogens were also evaluated. The seed extract was found to have high antibacterial activity. The total polyphenolic content of the ethanolic extract of S. cumini seed was determined by Folin-Ciocalteau method. The antioxidant activity of S. cumini seed ethanolic extract was evaluated by DPPH free radical scavenging assay, reducing power assay and total antioxidant capacity. S. cumini seed extract was found to have very high and potent antioxidant activity and might be due to the presence of high phenolic components in seed. The cytotoxicity assay of S. cumini seed ethanolic...
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