Posted by admin on May 1, 2011 in |
A simple and sensitive extractive spectrophotometric method has been described for the assay of Mianserin hydrochloride (M-HCl) either in pure form or in pharmaceutical solid dosage form. The developed method involves formation of colored chloroform extractable ion-association complex of Mianserin hydrochloride (M-HCl) with Picric acid (PA), Chlorophenol red (ClPR), Bromthymol blue (BrTB), Bromcresol purple (BrCP) reagents. The extracted complexes showed absorbance maxima at optimum wavelength using visible spectrophotometer. Beer’s law is obeyed in the concentration range of 1-42 μg/mL. Correlation coefficient was found to be ≥ 0.9985. In addition we have determined the molar absorptivity, Sandell sensitivity and the optimum conditions for quantitative analysis of the investigated...
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Posted by admin on May 1, 2011 in |
The World Health Organization estimates that a staggering two billion people harbor parasitic worm infections. Parasitic worms also infect livestock and crops, affecting food production with a resultant economic impact. Despite this prevalence of parasitic infections, the research on antihelmentic drug is poor. As per WHO, only few drugs are frequently used in the treatment of helminthes in human beings. Antihelmentic from the natural sources may play a key role in the treatment of these parasitic infections. In view of this petroleum ether (60-80º C) and ethanolic extracts of aerial parts of Enicostemma littorale Blume were evaluated separately for the activity on adult Indian earthworms, Pheretima posthuma, using albendazole as reference standards. Five concentrations (2.5, 5, 10, 25 & 50 mg/ml) of each extracts were studied in activity, which involved the determination of time of paralysis and time of death of the worm. The results indicated that the ethanolic extract was more potent than the petroleum ether...
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Posted by admin on May 1, 2011 in |
Withania somnifera L. Dunal (Family: Solanaceae) is extensively used in traditional Indian medicine as a nerve tonic and is thought to improve memory. We assessed the acetylcholinesterase (AChE) inhibitory activity and kinetics of an aqueous extract of W. somnifera root, in the context of its potential role in regulating the levels of acetylcholine (ACh) in the brain. The assessment of cholinesterase inhibition was carried out using a colorimetric method based on Ellman’s reaction. Our findings demonstrate that W. somnifera extract significantly inhibited AChE in a concentration-dependent manner. A 1mg/mL extract concentration resulted in an 86% inhibition as compared with control. The IC50 value was 0.15mg/mL calculated from the equation of the percentage inhibition curve for the test extract. The Lineweaver-Burk plot indicated that the extract followed mixed non-competitive inhibition kinetics. We conclude that the mode of action exhibited by the aqueous extract of W. somnifera root has a potential therapeutic application that might be beneficial in improving memory and other cognitive functions associated with the cholinergic...
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Posted by admin on May 1, 2011 in |
In the present study we have explored the effect of Urtica dioica, a traditional antidiabetic herb used in India and Bangladesh, on insulinemic and chronic inflammatory status of type 1 diabetic model rats. The mature and fresh leaves of U. dioica was collected from the mountain range of Assam in India and the water extract of the leaves was used at a dose of 1.25g/kg body weight. Adult Long-Evans male rats, bred at BIRDEM Animal house, were used in this study. Type 1 DM was produced with single intraperitoneal injection of Streptozotocin using standardized methods. The experiment for semi-chronic effects was done with continuous feeding of water extract of U. dioica for 8 days. Serum glucose estimated by glucose oxidase (GOD-POD) method, serum insulin was measured by ELISA and CRP (as a marker for chronic inflammation) was also measured by an ELISA technique specific for rats. Serum lipids were measured by enzymatic-colorimetric methods. When compared to Control the water extract of U. dioica showed a significant hypoglycemic effect on...
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Posted by admin on May 1, 2011 in |
Ampicillin is used to treat variety of Bacterial infections in Humans. Piperine, an alkaloid from Piper species is reported to enhance oral bioavailability of co-administered drugs. The present study was aimed to include to Piperine (bioenhancer) as a formulation additive in oral formulations of Ampicillin Trihydrate. So, a Physical mixture of Ampicillin Trihydrate and Piperine (1:1) was tested for their compatibility by DSC, TLC and FT-IR techniques. Stability of the mixture was assessed by the use of ICH guidelines. The above studies have proved that Piperine can be used as a formulation additive (bioenhancer), in oral formulations of Ampicillin...
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