Posted by admin on Apr 1, 2011 in |
Solid dispersions traditionally have been used as effective methods to improve the dissolution properties and bioavailability of poorly water-soluble drugs. Furosemide a loop diuretic belonging to Biopharmaceutical Classification System (BCS) Class IV, has very poor water solubility. The aim of the present study was to improve the solubility and dissolution rate of a poorly water-soluble drug, furosemide, by solid dispersion technique as well as to evaluate the potential of Eudragit RL-100, RS-100 and Drug coat L -100, S -100 (methacrylic acid polymers) as carriers for solid dispersions. Solid dispersions were prepared by solvent evaporation technique. The solid dispersions were characterized for particle size, particle size distribution, solubility studies and interaction studies such as FTIR spectroscopy. Solid state characterizations i.e., X-Ray diffraction study; Differential Scanning Calorimetry and Scanning Electron Microscopy were also carried out for the best formulation. In contrast to the very slow dissolution rate of pure furosemide, the dispersion of the drug in the polymer considerably enhanced solubility. The aqueous solubility of furosemide was favoured by formulating it...
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Posted by admin on Apr 1, 2011 in |
Three Simple, precise, accurate and economical UV methods have been developed and validated for the quantitative estimation of Dorzolamide HCl in bulk and pharmaceutical dosage form. Dorzolamide HCl has the absorbance maxima at 253 nm in zero order spectra (Method A). In the first order derivative spectra, showed absorbance maxima at 238 nm (Method B) and in the second order derivative spectra, showed peak maxima at 278 nm (Method C). Distilled water was used as solvent for all the methods. Beer’s law was found to be obeyed in the concentration range of 3-24 μg/ml. The developed method was validated according to ICH guidelines and was found to be accurate, economic and precise. The proposed method can be successfully applied for the estimation of Dorzolamide HCl in bulk and pharmaceutical dosage...
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Posted by admin on Apr 1, 2011 in |
Repaglinide is widely used as a hypoglycemic drug for the treatment of chronic type 2 diabetes mellitus. The objective of the present study was to improve the bioavailability of repaglinide by nasal delivery using degradable starch microspheres. Degradable starch microspheres were prepared by an emulsion polymerization method and formaldehyde was used as a cross linking agent. The formulations were characterized for their encapsulation efficiency, surface morphology, particle size and ex vivo drug release pattern. The relative bioavailability in rats was studied in lead formulations. The repaglinide microspheres formulations showed significant increase in...
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Posted by admin on Apr 1, 2011 in |
The present study undertaken to predict the three dimensional structure of γ-Hexachlorocyclohexane dehydrochlorinase (linA) in Sphingomonas paucimobilis UT26. γ-Hexachlorocyclohexane dehydrochlorinase (linA) catalyzes the initial steps in the biotransformation of the important insecticide γ-hexachlorocyclohexane (γ-HCH) by the soil bacterium Sphingomonas paucimobilisUT26. Stereo chemical analysis of the reaction products formed during conversion of γ-HCH by linA was identified from structural database using homology modeling or comparative modeling approach. Based on the knowledge of the template, a three-dimensional model was predicted and processed in to energy minimization, Ramachandran plot analysis, quality assessment and finally deposited into Protein Model...
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Posted by admin on Apr 1, 2011 in |
The methanolic extract along with some organic soluble fractions of the bark of Dillenia indica Linn. were tested against four Gram-positive and seven Gram-negative bacteria and against three pathogenic fungi. n-Hexane and dichloromethane fractions showed remarkable activities against all the tested bacteria but n-Hexane fraction showed highest activity against Shigella dysenteriae and its zone of inhibition was 15.51 ± 0.75 mm. Other showed moderate or little activity. Methanol crude extract showed highest activity against fungus Candida albicans with a zone of inhibition 13.13±1.75mm. Lowest minimum inhibitory concentration (MIC) values were observed in n-hexane fraction against Shigella dysenteriae and Staphylococcus aureus and were 0.312 in both cases. Lowest LC50 value 19.02±1.16 of n-hexane fraction indicated the highest toxicity in comparison with the other...
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