Posted by admin on Jul 1, 2011 in |
Antibacterial, antifungal and phytotoxic activities of the prepared pyrazine derivatives of benzil (A), 4, 4’-dibromobenzil (B) and α-diketones of triterpenoids skeleton e.g. 2, 3-diketolupene (C) and 2, 3-diketomethyl- dihydrobetulonate (D) were studied. The prepared 1, 4-pyrazine derivatives (E, F, G & H respectively) have been characterized by UV, IR, NMR (1H and 13C), optical rotation, mass spectra and by comparison with authentic samples. Disc diffusion technique was used to determine in vitro antibacterial and antifungal activities. Phytotoxicity was determined against seed growth technique. In addition, minimum inhibitory concentration (MIC) was determined using serial dilution technique to determine antibacterial potency. The derivatives showed significant antimicrobial activities against Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa (bacterial specimen), Colletotrichum camelliae, Fusarium equisiti, Alternaria alternata, Curvularia eragrostidis and Colletotrichum gloeosporioides (fungal...
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Posted by admin on Jul 1, 2011 in |
The present work describes a reverse phase high performance liquid chromatographic method without use of buffer in mobile phase for the simultaneous estimation of Lornoxicam and Paracetamol in combined tablet dosage form. Chromatography was performed on Chromasil C18 (250 × 4.6 mm i.d. and particle size 5 μm) column in isocratic mode with mobile phase containing acetonitrile: 0.2% triethylamine aqueous solution (adjusted to pH 6.54 using 1 % ortho phosphoric acid) in the ratio of 62:38 v/v. The flow rate was 0.8 ml/min and effluents were monitored at 310 nm. The selected chromatographic conditions were found to be useful in separating Lornoxicam (run time 4.00 min) and Paracetamol (run time 3.05 min). Linearity for Lornoxicam and Paracetamol was found to be in the range of 2-14 μg/ml and 250-875 μg/ml with correlation coefficient of 0.9995 and 0.9991 respectively. Percent recovery of the drugs was found to lie between 99.06-99.64 % and 99.58-100.65 % for Lornoxicam and Paracetamol respectively. The proposed method was validated by different parameters. It was found...
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Posted by admin on Jul 1, 2011 in |
A reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of atorvastatin calcium and ubidecarenone in tablet formulation. The separation was achieved by 250×4.6 mm, 5µ, L-7 pack (peerless C-8) column and Methanol : Acetonitrile (80:20) as mobile phase, at a flow rate of 1.5 ml/min. The objective of this validation of an analytical procedure is to demonstrate that the drug Atorvastatin calcium and Ubidecarenone is suitable for its intended purpose. Developed method was found to be accurate, precise, selective, economical and rapid for simultaneous estimation of atorvastatin calcium and ubidecarenone in...
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Posted by admin on Jul 1, 2011 in |
The aim of this study was to determine the antimicrobial activity of three selected Lebanese plants (Rheum rhaponticum, Olea europaea, and Viola Odorata) against Extended Spectrum Beta Lactamase (ESBL) – producing Escherichia coli and Klebsiella pneumoniae, and to identify the specific plant fraction responsible for the antimicrobial activity. The plants were extracted with ethanol to yield the crude extract which was further subfractionated by different solvents to obtain the petroleum ether, the dichloromethane, the ethyl acetate and the aqueous fractions. The Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) were determined using broth microdilution. The MICs ranged between 2.5 and 80 μg/μl. The majority of these microorganisms were inhibited by 80 and 40 μg/μl of the crude extracts. The dichloromethane fraction of Olea europea exerted a significant inhibitory effect on 90% of the tested strains. Ethyl acetate extracts of all selected plants presented antibacterial activity with high potency. Aqueous extracts of Rheum rhaponticum and Olea europaea exerted antimicrobial activity against the majority of the tested strains while Viola...
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Posted by admin on Jul 1, 2011 in |
The study was undertaken to investigate the hypolipidemic and hepatoprotective effects of the different fractions (Petroleum ether, ethyl acetate and chloroform) of ethanolic extract of Brassica oleracea. The different fractions of extract were administered intraperitoneally as a single dose of 150 mg/kg body weight to alloxan induced diabetic rats and found to reduce blood lipid level (Total cholesterol and Triglycerides) significantly (p<0.05). The plant fractions also exhibited correction of altered biochemical parameters viz., SGOT and SGPT levels in diabetic rats. The effect of plant fractions were compared with standard drug metformin. The phytochemical screening tests indicated that the different constituents such as saponins, tannins, triterpenes, alkaloids and flavonoids etc. were present in the plant which has hypolipidemic and hepatoprotective properties. Thus, this study indicates that various fractions (Petroleum ether, ethyl acetate and chloroform) of the ethanolic extract of Brassica oleracea have favorable effect in hypolipidemic and hepatoprotective...
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