Posted by admin on Jan 1, 2011 in |
Nigella sativa L. (Black cumin) has showed a broad spectrum of pharmacological effects like antipyretic, analgesic, anti-inflammatory and antimicrobial. These activities may be related to the seeds oil. The most important chemical composition of hexanic extract of seeds was unsaturated fatty acids and essential oil. In this investigation the hexanic extract of seeds was topically applied to evaluate the healing efficiency of seeds oil on the second degree burn wound models in rats. Animal were randomly divided into three groups of six for each group. Silver sulfadiazine was applied as an antiseptic standard drug. Wound healing was assessed by the rate of contraction and histological characteristics in treated and untreated groups. On day 12, the extract-treated animals showed 81.20% reduction in the wound area and were significantly (P<0.05) more than control group 63.31%. Histological study showed fully grown regenerated epidermis on day 12 in treated rats. The present study suggests the burn wound healing action of seeds may be due to anti-inflammatory, antioxidant and antimicrobial activities of major compounds...
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Posted by admin on Jan 1, 2011 in |
Psidium guineense Swartz is a shrub native to Brazil. It is consumed in natura or in the form of pulp, which presents a high content of C vitamin and proteins. The chemical prospection of the aqueous extracts from unripe and mature pulp revealed the presence of tannins and flavonoids. The extracts demonstrated moderate antibacterial activity against the most of strains tested, and the best result was obtained with the unripe pulp extract against Staphylococcus aureus (MR358; MIC 256 μg/mL). The extracts were further tested for in vivo brine shrimp lethality test. The mature pulp extract exhibited significant toxicity (LC50=125...
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Posted by admin on Jan 1, 2011 in |
The aim of the present study was to carry out phytochemical screening and anticonvulsant activity of various extracts of the whole parts of Wedelia chinensis. The extracts (Petroleum ether, Ethanolic & Aqueous) were subjected to various chemical tests in order to identify the main phytoconstituents of the plant. The results of the present investigation revealed that the ethanolic extract contains glycosides and little amount of alkaloids and flavonoids, petroleum ether extract indicates the presence of steroids and aqueous extract was rich in glycosides and saponins. The anticonvulsant activity of ethanolic and aqueous extract of whole plant of Wedelia chinensis at a dose level of 250, 500, 750 mg/kg b.w, p.o. was performed in mice by using MES and PTZ methods. Phenytoin was used as a reference compound (25mg/kg b.w, i.p). Seizures were induced by delivering electroshock of 50 mA for 2 seconds by means of electro convulsiometer through a pair of ear clip electrodes whereas in PTZ model, PTZ at the dose of 80 mg/kg was injected i.p. to...
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Posted by admin on Dec 1, 2010 in |
A simple and sensitive liquid chromatography–tandem mass spectrometry method was developed and validated for estimation of Labetalol in human plasma, an adrenergic β-receptor blocking agent used in the treatment of hypertension, which exhibits both α- and β-adrenoreceptor blocking activity. The analyte and internal standard (Metoprolol) were extracted by liquid/liquid extraction with ethyl acetate. The chromatographic separation was performed on reverse phase Phenomenax Luna column (C18, 5µm, 100 x 4.6 mm) with a mobile phase of 2 mM ammonium formate (pH 5.0) / methanol (20:80 v/v), which was pumped at a flow rate of 0.5 mL / min with split ratio of 20:80. The protonated analyte was quantitated in positive ionization by multiple reactions monitoring with a mass spectrometer. The mass transitions m/z 329.01→161.95 and 267.99→115.86 were used to measure Labetalol and Metoprolol, respectively. The lower limit of quantitation was 3.1800 ng/mL with a relative standard deviation of less than 15%. Acceptable precision and accuracy were obtained for concentrations over the calibration curve ranges (3.1800 to 700.8760 ng/ml). Sample analysis...
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Posted by admin on Dec 1, 2010 in |
Aceclofenac is an analgesic and anti-inflammatory agent used in the management of osteoarthritis, rheumatoid arthritis and ankylosing spondylitis. The objective of the present work was to investigate the effect of different types of carriers such as polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG) 6000 and sodium lauryl sulphate (SLS) as solubilizer on in vitro dissolution of aceclofenac. Aceclofenac solid dispersions were prepared using 3² factorial design by fusion and solvent evaporation method with PEG 6000, PVP & SLS. Prepared aceclofenac solid dispersions were evaluated for physical appearance, drug content uniformity, and in vitro dissolution studies. The dissolution was determined by USP XXIII apparatus using phosphate buffer pH 7.4. The highest aceclofenac dissolution rate, 99.87% in 60 minutes, was obtained from solid dispersion containing SLS (ASS7) prepared by solvent evaporation method. The general trend indicated that there was an increase in dissolution rate for solid dispersions prepared in following order SLS>PVP>PEG 6000. IR and DSC studies showed no chemical change between drug and polymer and aceclofenac is homogeneously distributed in an...
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