Posted by admin on Feb 1, 2011 in |
A simple high-performance liquid chromatography RP-HPLC and two reproducible ultraviolet spectrophotometric methods were developed and validated for the estimation of nebivolol HCl and valsartan simultaneously in combined pharmaceutical solid dosage form. The wavelength fixed for spectrophotometric measurement of valsartan at 246.6 nm, nebivolol HCl at 280.2 nm for the simultaneous equation and for the Q-analysis measurement the two wavelengths fixed are 246.6 nm and 275.0 nm. The linearity obtained for nebivolol HCl in the range of 0.5-2.5 µg/ml and 5-25 µg/ml for valsartan by spectrophotometric method. The nebivolol HCl and valsartan were analyzed by HPLC using reverse phase C18 column (25 cm x 4.6 mm i.d., 5 µm particle size), by isocratic mobile phase consisted of 50mM ammonium acetate buffer adjusted to pH 3.5 and acetonitrile (30:70 v/v), at a flow rate of 0.8 ml/min in the detection wavelength of 275 nm. The loratidine was used as an internal standard. The linearity and range of nebivolol HCl are 2-12 µg/ml and 5-30 µg/ml for valsartan by HPLC method. The...
Read More
Posted by admin on Feb 1, 2011 in |
The methanolic extract of Nerium indicum showed the presence of alkaloids, tannins, phenols, and starch in phyto-chemical investigation. Total phenolic content of the drug was estimated by Folin Ciocatteu’s method and was found to be 55.20 µg/10 ml. Analgesic activity was performed using three models they are Writhing, Tail-flick activity and Hot plate models. The methanoilc extract showed 44.51% inhibition in Writhing model, 50.09% for 30 sec’s and 62.45% in 60 sec and hot plate method showed 47.31%. Analgesic activity was carried out and the results were encouraging, which stated that the methanolic extract of Nerium indicum showed significant peripheral analgesic activity. The mechanism of analgesic activity action of Nerium indicum may be due to its inhibitiory effect on the synthesis of prostaglandins and...
Read More
Posted by admin on Feb 1, 2011 in |
In the present study a new series of coumarin derivatives have been synthesized by condensation of ethyl acetoacetate and resorcinol. The chemical structures of the synthesized compounds were confirmed by means of IR, 1H-NMR and Mass spectral analysis. These compounds were screened for their Analgesic and Anti-inflammatory activities. Among the synthesized compounds II (a-1 to...
Read More
Posted by admin on Feb 1, 2011 in |
The study was undertaken to investigate the antihyperglycemic, oral glucose tolerance test (OGTT) and glycogenesis effects of the different fractions (Petroleum ether, ethyl acetate and chloroform) of methanolic extract of Momordica charantia. The different fractions of the extract were administered intraperitoneally as a single dose of 150 mg/kg body weight to alloxan induced as well as glucose induced diabetic rats and found to reduce blood glucose level significantly (p<0.05). The different fractions of Momordica charantia to the alloxan-induced diabetic rats resulted in the significant elevation of liver glycogen content which was decreased by 50.60% in diabetic control. The plant fractions also improve the glucose tolerance in the glucose induced rats. The effects of plant fractions were compared with standard drug metformin. The phytochemical screening tests indicated that the different constituents such as saponins, tannins, triterpines, alkaloids and flavonoids etc. were present in the plant which has antidiabetic and glycogenesis properties. Thus, this investigation paves the way for plant based diabetic treatment and indicates that various fractions (Petroleum ether, ethyl...
Read More
Posted by admin on Feb 1, 2011 in |
The direct delivery of therapeutically active drug to the pulmonary tract has proved to be a life saving tool for many Asthmatics. Salbutamol being a drug of choice in severe Asthma attacks, in the present study attempt was made to prepare MDI of Salbutamol in suspension formulation and compare it with the marketed MDI of salbutamol suspension. The proportion of C.F.C. propellant blend (p11 & p12) in 30:70 proportion gave optimum vapor pressure; in the range of 70-75 psig was selected. Oleic acid was selected as a surfactant and its concentration was optimized. A series of physicochemical tests were performed simultaneously on both the preparations. Results were compared. The formulations were further subjected to in-vitro evaluation whereby net respirable fraction was determined. Logic behind this study was to create a better suspension than that available in the market. In all qualitative as well as quantitative tests the formulated suspension was comparable with marketed MDI. However our formulation retained minimum amount of drug (8.57%) on adaptor compared to the marketed...
Read More
