Posted by admin on Dec 1, 2010 in |
In the present study, Solanum melongena Linn. (Brinjal; Solanaceae), was evaluated for its antiepileptic activity against experimental seizures. The roots ethanolic extract of Solanum melongena protected mice against tonic convulsion induced by maximal electroshock, Pentylenetetrazole and Picrotoxin. It was found that ethanolic extract up to a dose of 300 mg/kg p.o. decreased the duration of tonic extensor phase and delayed the onset of time of jerk against maximal electroshock induced seizure and pentylenetetrazole induced seizure. In Picrotoxin induced seizure, the extract at a dose of 200 mg/kg significantly increased the onset of clonic- tonic convulsion. These findings suggest that extract of the roots of Solanum melongena posses anticonvulsant effect and reduce mortality. The further studies to determine the mechanism(s) and compound(s) involved in the anticonvulsant...
Read More
Posted by admin on Dec 1, 2010 in |
A simple, rapid and accurate reverse phase high-performance liquid chromatographic method for the determination of 5-Flurouracil (5-FU) in pharmaceutical formulations and human plasma samples has been developed and validated. The assay of the drug was performed on a CLC C18 (5 μm, 25 cm 4.6 mm i.d.) with UV detection at 266 nm. The mobile phase consisted of methanol-water mixture in the ratio of 98:2, and a flow rate of 1 ml/min was maintained. The standard curve was linear over the range of 0.9-18.4 µg/ml (r2=0.9966). Analytic parameters have been evaluated. Within-day and between-day precision as expressed by relative standard deviation was found to be less than 2%. The method has been applied successfully for the determination of 5-FU in spiked human plasma samples and pharmaceutical formulations. The method will be useful for routine quality control...
Read More
Posted by admin on Dec 1, 2010 in |
Antioxidant activity of methanolic extract of Tectona grandis root was investigated for its free radical scavenging activity by determining the nitric oxide and superoxide radical scavenging activity. Maximum scavenging of nitric oxide and superoxide radical found were 26.61% and 46.64% respectively at 250 μg/ml concentration. The results were compared with rutin as a standard. These results clearly indicate that Tectona grandis is effective in scavenging free radicals and has the potential to be a powerful...
Read More
Posted by admin on Dec 1, 2010 in |
In the present research investigation we extracted the powdered rhizome of Drynaria quercifolia linn by Soxhletion method using different solvents. Then extracts were subjected to preliminary phyto-chemical investigation. The proximate analysis was carried out for the plant rhizome powder. The total ash value was 9.93%, Acid insoluble ash value was 4.49%, and Water-soluble ash value was 6.96% and extractive values of alcohol and water was found to be 9.87% and 13.94%. The materials were subjected to successive extraction with solvents. The solvents used were petroleum ether, chloroform, methanol and water in the ascending order of polarity. Pet ether extract was Light brown color, highly viscous and sticky and the yield was 3.12%, chloroform extract was brownish black color, viscous and sticky and the yield was 5.72%, methanol extract was brown color and sticky and yield was 19.67%, and the water extract was brown and sticky and yield was 16.33%. All the extract was subjected for qualitative chemical evaluation to detect the phyto-constituents present in them. Pet ether extract revealed...
Read More
Posted by admin on Dec 1, 2010 in |
The aim of present study was to investigate the phytochemical screening and to compare the antimicrobial activity of oils of Clove bud and Cardamom. Clove bud was successively extracted by steam distillation and isolated with Dichloromethane. The phytochemical analysis revealed the presence of alkaloids, glycoside, steroids, carbohydrates, terpenoids, tannins and phenolic compound. The dichloromethane extract was chromatographed over silica Gel (60-120) and eluted with pure toluene, toluene: Dichloromethane (9:1), toluene: Dichloromethane (8:2), toluene: Dichloromethane (7:3), fraction were monitored by T.L.C. similar fractions were combined and concentrated .eleven fractions were obtained and were labelled as f1, f2, f3 to f11. Cardamom fruit was successively extracted with petroleum ether. The phytochemical analysis revealed the presence of alkaloids, glycoside, steroids, protein, carbohydrates, terpenoids, tannins and phenolic compound. The Petroleum ether extract was chromatographed over silica Gel (60-120) and eluted with pure Benzene, Benzene: chloroform (9:1), Benzene: chloroform (8:2), Benzene: chloroform (7:3), Benzene: chloroform (6:4), Benzene: chloroform (5:5), Benzene: chloroform (4:6), and with pure chloroform. Fractions were monitored by T.L.C. similar fractions were...
Read More
