Posted by admin on Jun 30, 2023 in |
The present study focuses on developing and validating a high-performance thin-layer chromatography (HPTLC) densitometry method for identifying and quantifying ellagic acid, glycyrrhizic acid and hesperidin in the ethanolic extract of Punica granatum, Glycyrrhizic glabra and Citrus sinensis. The separation was achieved by mobile phase for ellagic acid was Methanol: Water: Formic acid (3.8:4.7:0.8) and spotting was done on RP TLC Al plate silica gel 60, for glycyrrhizic acid was ethyl acetate: Formic acid: Glacial acetic acid: Water (14.7:0.8:0.5:1) and mobile phase for hesperidin was n – butanol: Acetic acid: Water (2.8:0.6:0.9) on TLC aluminium plate pre-coated with silica gel 60 F254. Densitometric scanning was carried out at a wavelength of 254nm and 287nm in absorbance mode. The peak corresponding to reference standard and that of the extract had the same RF of 0.19 for ellagic acid, 0.14 for glycyrrhizic acid and 0.58 for hesperidin. Following the International Conference of Harmonization (ICH) requirements for precision, accuracy, and reproducibility, the method was verified. The regression equation showed a linear function with...
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Posted by admin on Jun 30, 2023 in |
Understanding the preponderance of respiratory conditions with the symptoms like sore throat and cough being even more common, owing to the coronavirus outbreak. The improved resolution method was developed to quantify drugs Loratidine and Ambroxol from the most effective dosage regimen to treat sore throat and cough. An accurate, precise method was developed for concurrently estimating drugs from combined tablet dosage form by first-order derivative UV spectrophotometric method. Absorption maxima were determined as 265 nm for Ambroxol hydrochloride (ZCP for Loratadine) and 307 nm for Loratadine (ZCP for ambroxol hydrochloride), using 0.1 N HCl as a solvent for estimation. The analysis results were developed as per ICH guidelines and the drugs obeyed beer lambert’s law in a concentration range of 10 – 125 µg/mL for Ambroxol hydrochloride and 2 – 12 µg/mL for Loratadine with a regression coefficient of 0.9994 and 0.999 respectively. The %RSD values (<2) in precision studies indicate the method’s reproducibility. The LOD values were found to be 1.35µg/ml and 0.37µg/mL, and LOQ values were found...
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Posted by admin on Jun 30, 2023 in |
This entire study is based on the drug delivery system of the pharmaceutical company to design the drugs. In order to identify the effectiveness of the drug, this study has tried to identify the bonds between peptides & proteins. In this context, this study has chosen the drug acetazolamide and based on the chemical properties, it has made a calibration curve of the drugs in water solution. In addition, this study has tried to analyze the recompression factors to identify the percentages of drug release in relevant time...
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Posted by admin on Jun 30, 2023 in |
Marine microorganisms are a valuable source of novel enzymes with ideal characteristics because of the halophilic nature of marine bacteria. The present study aims to isolate, screen, and identify the marine bacterial strains from marine sediment samples that produce an enzyme urease with clinical and industrial applications. Marine sediment samples were collected and cultured on zobell marine agar medium. After incubation seven bacterial strains were isolated from the culture. From this, five isolates were showed urease activity on the urea agar base medium. Morphological and biochemical test of urease-producing organisms was done. Urease-producing bacterial strains (UR S7B) were selected on the basis of their urease test. The selected strain UR S7B was further sequenced for identification. The phylogenetic characterization and 16S rRNA of the strain UR S7B revealed that bacterial cultures belong to Bacillus sp. The morphological studies indicated that the isolate was Gram -ve, rod-shaped and motile organism. The present study concludes that marine bacteria can be a urease source with important...
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Posted by admin on Jun 30, 2023 in |
A cross-sectional study was conducted among 140 study subjects in an urban residential area of East Delhi selected by simple random sampling method to assess the prevalence of self-medication for Covid-19 and associated socio-demographic and economic factors. Data were collected by interviewing the study subjects using a pre-tested semi-structured interview schedule. The Mean+SD age of the study subjects was 41.89+13.54 years (range: 20 to 75 years); 77 (55.0%) were males, and 75.7% were graduates and above. There were 86 with a Covid-19 positivity history. The prevalence of self-medication among them was 33.7% (95% CI: 23.9%, 44.7%), least in 30-39 years (5.9%, 95% CI: 0.1%, 28.7%), maximum in 40-49 years (44.4%, 95% CI: 21.5%, 69.2%) (p=0.101), higher in females (38.1%, 95% CI: 23.6%, 54.4%) than males (29.5%, 95% CI: 16.7%, 45.2%) (p=0.402), socioeconomic class III (52.4%, 95% CI: 29.8%, 74.3%) and least among class I (20.0%, 95% CI: 9.6%, 34.6%) (p=0.017). Self-medication was not associated with education level, working status and marital status. Prevalence of self-medication was high during the...
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