Posted by admin on Sep 1, 2010 in |
Thymol is the main volatile compound extracted from dried fruits of Tachyspermum ammi Mill. The compound serves as a biological marker because of its interesting biological activities and is used in traditional medicine, as a bactericide, fungicide, Gastro-intestinal disorders, Bronchitis and asthma, and others. A GC method for the determination of thymol was developed. Chromatographic analysis was carried out on – stainless steel 30m×0.25m×0.25mm packed with polysiloxane column with flow rate 1.0 ml per minute of the carrier gas. Quantification was performed using a FID. The method was validated for specificity, precision, Linearity, accuracy and robustness. The method was found to be precise for different concentrations of thymol. Accuracy was checked by conducting recovery at different level of thymol and the average percentage and recovery was found to be in the range 90%-110%. The Linearity was established over a range of seven different concentrations of the analyte (70%, 80%, 90%, 100%, 110%, 120% and 130% of test concentration) with correlation coefficients of 0.9997 for thymol....
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Posted by admin on Sep 1, 2010 in |
Diabetes mellitus is a disorder of metabolism resulting from insulin abnormality. Tissue levels of chloroquine in diabetes were investigated. Few diseases have such plural effects in the body as diabetes mellitus. Chloroquine distribution in the tissue and blood was investigated in diabetic rats to a certain how diabetes mellitus affects the levels of chloroquine in tissues and blood. This may prove valuable in a possible diabetes- malaria case treatment. Diabetes mellitus was induced in a group of experimental rats using streptozotocin, 60mg/Kg dose. The mean volume of urine passed over 96 hour period after 10 days of streptozotocin administration was 31.74±0.06 ml and that of control rats was 23.28±0.19 ml. The values showed significant statistical difference (p<0.05). Confirmation of diabetes was performed 14 days after streptozotocin administration by determining the blood glucose levels, using glucose oxidase reagent. The diabetic and the control rats were divided into two groups each, which were given 10mg/Kg and 20mg/Kg single dose of chloroquine respectively. 96 hours after the administration of a single dose...
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Posted by admin on Sep 1, 2010 in |
A series of novel N-substituted benzimidazole derivatives were synthesized and screened anti-viral activity against a HIV -1 and -2 in MT-4 cells. New compounds were synthesized through modifying the N-1 hydrogen of benzimidazole moiety with the substitution of sulphanilamide, sulphadimidine, sulphamethoxazole, 2-aminopyridine, pthalamide, benzamide, nicotinamide, anthranilic acid and 2- marcapto- benzimidazole by mannich reaction. The structure of the synthetic compounds was characterized by means of IR and PMR data. The anti-HIV activities of the new compounds were also screened for in vitro anti-viral activity against replication of HIV-1 (IIIB) and HIV-2 (ROD) in MT-4 cells using AZT- as standard and cytostatic activity were also studied by MT- 4/MTT assay. Benzimidazole derivative BSD inhibited the replication of HIV-1 and 2 (EC50= 35.40µg/ml and CC50>125µg/ml) in MT-4...
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Posted by admin on Sep 1, 2010 in |
Keywords: Pterospermum acerifolium, Pharmacognosy, Sterculiaceae, Drug StandardizationPterospermum acerifolium plant is considered to be laxative, anthelmintic, stomachic and used in inflammation, blood disorders, ulcers and leprosy. The leaf was studied for pharmaconostic evaluations, including examination of morphological and microscopic characters, determination of leaf constants, ash values and extractive values. The morphological studies revealed that the leaf is dark green color with a glabrescent texture and characteristic odour. In the microscopic studies, the leaves showed the presence of long, lignified stellate and four armed trichomes, anomocytic and paracytic stomata, 2 layers of radially elongated palisade cells below upper epidermis, collenchyma, vascular bundles and spongy parenchyma. The total ash, acid insoluble ash and water-soluble ash values were observed to be 4%, 1.4% and 2.5% respectively. The leaves were successively extracted with Petroleum ether, chloroform, ethyl acetate, methanol and water in increasing order of polarity. Preliminary phytochemical investigation showed the presence of alkaloids, glycosides, tannins, triterpenoids, carbohydrates and flavonoids....
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Posted by admin on Sep 1, 2010 in |
In recent times, focus on plant research has increased all over the world and a large body of evidence has collected to show immense potential of medicinal plants used in various traditional systems. Solanum nigrum (commonly called Makoi) is also a medicinal plant. A dietary intake of Solanum nigrum supplies our body with nutrients that offer protection against numerous diseases. All parts of this plant are used in the traditional medicine as a remedy for treating various diseases like, cough, cold, asthma, skin diseases and liver problem. Now a day, Diabetes mellitus has become a real problem of public health in developing countries. This is a metabolic disorder characterized by disarrangements in carbohydrate, protein and fat metabolism caused by the complete or relative insufficiency of insulin secretion and /or action. This is due to defective or deficient insulin secretary response. This results into impaired glucose use, which is a characteristic feature of diabetes mellitus i.e. resultant hyperglycemia. Elevated blood glucose level causes dehydration of the tissue cells as glucose...
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