Posted by admin on Feb 1, 2011 in |
A simple, precise, specific and accurate reverse phase HPLC method has been developed for the determination of Ritonavir in bulk and pharmaceutical dosage forms. The chromatographic separation was achieved on Symmetry C18 (4.6 x 100mm, 3.5 μm) column using a mixture of Buffer: Acetonitrile (50:50) as the mobile phase at a flow rate 1.0 ml/min. Linearity was observed in concentration range of 50-150μg/ml. The retention time of Ritonavir was 5.1 min. The analyte was monitored using UV detector at 239 nm. Results of analysis were validated statistically and by recovery studies. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and...
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Posted by admin on Feb 1, 2011 in |
A considerable number of studies have already been performed involving the roots and stems of Berberis aristata and thus the following investigation has been carried out for the phytochemical study of ethanolic extract of heartwood of Berberis aristata. The heartwood (1.8 kg) of Berberis aristata DC. Var aristata (Berberidaceae) was exhaustively extracted in 95% ethanol using Soxhlet apparatus. The column was prepared for isolating various phytoconstituents using solvents of increasing polarity. On the basis of various spectral data analysis i.e., IR, 1HNMR, 13CNMR and +ve ion FAB MS, one of the isolated phytoconstituents was characterized as an aliphatic hydrocarbon,...
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Posted by admin on Feb 1, 2011 in |
Purpose: A study was conducted for Arthritis, mainly focused on Osteoarthritis (OA) and Rheumatoid Arthritis (RA), to check the prevalence, awareness, severity, probable causes, drug utilization and evaluate efficiency of different therapies in population of North Gujarat. Methodology: Detailed questionnaires were designed to collect data randomly from 260 patients of rural and urban area of Palanpur and Mehsana districts. Results: Prevalence of OA was found to be 3.11% where as that of RA was 0.06%. Furthermore, the prevalence ratio (male: female) for OA was 1:1.19, where that of for RA was 1:4. Prevalence of OA and RA is significantly higher in urban area than in rural area. Suffering for most arthritic patients is more than five years indicating its chronic nature. The usage of Allopathic medicine for the treatment for OA and RA is highest as compared to other therapies. Family history for OA and RA patients was found 20% and 28.57% respectively indicating hereditary factor for diseases. More numbers of parts affected in RA patients than that of...
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Posted by admin on Feb 1, 2011 in |
This study is concerned to develop a rapid system for regenerating shoots and callus from mature shoot tip explants of Asteracantha longifolia (L.) Nees, a medicinally important annual herb (family acanthaceae). Effective shoot and callus regeneration was observed by using several concentrations of cytokinins and auxins with MS medium. Shoot tips responded better for callusing almost in all combinations such as BA + IAA (1.0+1.0, 1.5+1.0, 1.5+1.5, 2.0+1.5 mg/lit) and BA + NAA (1.0+1.0, 1.5+1.0, 1.5+1.5, 2.0+1.5 mg/lit). Shooting was observed only in shoot tip explants when media was supplemented with growth regulators in different combinations at different concentrations such as BA (0.5, 1.0, 1.5, 2.0 mg/lit) and BA+NAA (1.0+0.5, 1.5+0.5, 1.0+1.0, 1.5+1.0). However no shooting was observed in MS media by using nodal explants containing different concentrations of BA +NAA + IAA. This approach of plant tissue culture may proved to be a helpful method to develop a rapid system of regeneration for production of medicinally important products. Also the production of aseptic plants which may decline the...
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Posted by admin on Feb 1, 2011 in |
A simple, rapid and reproducible HPLC method was developed and validated for the estimation of Formoterol Fumarate Rotacaps. Symmetry C8 (4.6×150mm, 5 µ) column, in isocratic mode with mobile phase containing Ammonium Acetate Buffer pH 5.0 and Acetonitrile (80:20) was used. The flow rate was 1.0 ml/min and the analyte was monitored at 254 nm. The retention time for Formoterol was about 4.6 mins. The system was validated for precision, accuracy, linearity, limit of detection and limit of quantitation. The Linearity was obtained in the concentration range of 30µg/ml to 180µg/ml with correlation coefficient of 0.999. The percentage recovery of Formoterol was found to be in the range of 98% -102%. Therefore it was concluded that the proposed method can be used for routine analysis of Formotero Fumarate Rota...
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