Posted by admin on Aug 1, 2010 in |
The non-proliferative activity of isolated saponins, gymnemagenol (C30H50O4) from Gymnema sylvestre and dasyscyphin C (C28H40O8) from Eclipta prostrata leaves were tested under on HepG2 cells under in vitro conditions. Both gymnemagenol and dasyscyphin C exhibited non-proliferative activity on HepG2 cells at 24 h with the IC50 value of 18.5 and 23.5μg/ml respectively. 5-Fluorouracil (5FU) a positive control showed the IC50 value of 1.34μg/ml. The isolated saponins were not toxic to Vero cells. From this study, it can be concluded that the saponins, gymnemagenol and dasyscyphin C have significant non-proliferative activity on HeLa cells under in vitro...
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Posted by admin on Jul 1, 2010 in |
Ondansetron is a serotonin 5HT3 antagonist; anti emetic drug. Bitter taste of the ondansetron is a major problem in ensuring patient compliance. Resinates of ondansetron were prepared with Indion 234 and loading process was optimized. A 3² factorial design was used for formulation development of orodispersible tablet of these taste masked resinates. The amount of subliming agent (camphor) and superdisintegrants (sodium starch glycolate) were taken as formulation variables (factors) for optimizing disintegration time, drug release after 15 minutes and friability as dependent or response variables. A mathematical model was generated for each response parameter. The disintegration time was found to linearly increase with the increase in the amount of superdisintegrants. The percentage friability showed no definite relationship with either amount of subliming agent or superdisintegrants. The optimum formulations were chosen by grid search method and their predicted results were found to be in close agreement with experimental...
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Posted by admin on Jul 1, 2010 in |
This study involves synthesis of 3- [4- aryl substituted- 3 – yl] – thiazolidin- 4 – one – 5- acetic acid by microwave oven induced synthesis method. Synthesized compounds were screened for anti-microbial activity by MIC and zone inhibition tests against various gram positive organism and gram negative organisms respectively. The microbiological assay is based upon a comparison of the inhibition of growth of micro-organism by measured concentration of the antibiotics to be examined with that produced by known concentration of a standard preparation of the antibiotic having a known...
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Posted by admin on Jul 1, 2010 in |
The present work deals with development and standardization of phytochemical screening for quantification of methanolic extract of medicinal plant stem bark of Anthocephalus cadamba (Roxb.)Miq. The scientific parameter is necessary to identify the exact plant material and to find its quality and purity. The present study deals with various, physical evaluation and preliminary phytochemical screening of various successive extracts such as qualitative chemical analysis and different spectroscopic study were carried out and the parameters were reported. HPTLC of extract shows the seven different peaks confirming that the seven compounds present in methanolic extract of Anthocephalus cadamba. The alkaloidal content of stem bark of the title plant has been determined. These studies indicated the possible information for correct identification and standardization of this plant...
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Posted by admin on Jul 1, 2010 in |
A novel method was developed for the determination of fluoroquinolones such as ciprofloxacin hydrochloride (CPL) and ofloxacin (OFX) by isocratic reverse phase-high performance liquid chromatography (RP-HPLC) coupled with UV detection. The developed method was rapid, accurate, reproducible, economical and sensitive for the simultaneous estimation of ciprofloxacin hydrochloride and ofloxacin. Fluoroquinolone antibiotics were separated on an analytical column (SIL 100Å, 125×4.6 mm ID) C18-RP, 5 µm, at ambient temperature. The mobile phase was consisted of Phosphate Buffer (15 mM): Methanol: Acetonitrile: TEA (66:24:10:1% v/v), pH was 6.5 (adjusted with 80% HCl), at a flow rate 1ml/min and sample injection volume was 20-µL. UV detection at 289 nm and retention time (RT) was 3.01 and 6.03min for ciprofloxacin hydrochloride and ofloxacin respectively. The method was validated in terms of stabilizing accuracy, precision (Intra/Inter-day), linearity, specificity, stability and sensitivity. The proposed method has been successfully applied for the analysis of marketed tablets and can be used for the routine analysis of formulations containing any one of the above drugs without any alteration...
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