Posted by admin on Apr 30, 2023 in |
Docking has become an indispensable method for predicting a molecule’s efficacy and toxicity. This technique is of great value in reducing the pain and suffering caused due to toxic drugs when tested in animals. Rheumatoid arthritis and the pain associated with the condition is a curse to mankind. In this research, the molecular property and toxicity predictions and evaluation of anti-arthritic and analgesic activities of L-Ascorbic acid derivatives were carried out using in-silico models. The molecular property and toxicity were predicted using the software called as OSIRIS. Molecular properties of compounds like ClogP, solubility, molecular weight and drug-likeness were evaluated. Toxicity parameters like mutagenicity, tumorigenicity, irritancy and reproductive effect were also examined. The anti-arthritic and analgesic activities were analyzed using CDOCKER. All the designed 10 molecules were docked with Hase enzyme for evaluation of anti-arthritic activity and opioid receptor for evaluation of analgesic activity. The results showed favourable ClogP, solubility, molecular weight, and drug-likeness values and excellent dock scores with toxicity profiles except for C4 and C6. Compound C10...
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Posted by admin on Apr 30, 2023 in |
A reverse phase high-performance liquid chromatographic bio analytical Technique (RP-HPLC) was developed and validated as per ICH 2019, US FDA 2018 guidelines for the quantification of amlodipine besylate, Rosuvastatin and Valsartan in human plasma using RP – C18 column. The mobile phase in composition (acetonitrile: water in the ratio 75:25% v/v, pH adjusted to 4.0 with acetic acid) was pumped and run at a flow rate of 0.8 ml/min, and for monitoring of eluents, a detector was set at wavelength 245 nm. The precipitation method was employed for the separation of the analyte from the plasma. A calibration curve was generated from the response and respective conc of standard solutions in the Concrange of LLOQ to ULOQ. The method was studied and validated per regulatory guidelines for selectivity, specificity, accuracy, precision, and stability study. Statistical data of the calibration curve of these drugs in the biological matrix was found within the prescribed limit. Results of accuracy and precision of quality control samples of these combined drugs were found (104.25...
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Posted by admin on Apr 30, 2023 in |
The analgesic activity of vilazodone in albino rats and mice was compared with the standard drug pentazocine and diclofenac, and its anti-inflammatory activity in albino rats was compared with the standard drug indomethacin. The doses of vilazodone used were 1.8mg/kg and 3.6mg/kg in albino rats and 2.6mg/kg and 5.2 mg/kg in albino mice, and the methods to screen analgesic activities were eddy’s hot plate method, tail flick method using radiant heat and acetic acid induced writhing method and formalin-induced peritonitis was used to screen anti-inflammatory activity. The data was analyzed statistically and results were expressed as numbers and percentages. For vilazodone dose of 1.8mg/kg and 3.6mg/kg using eddy’s hot plate method mean reaction time were 6.50± 0.288 and 5.70± 0.285, in tail flick method 6.50±0.288 and 6.45± 0.521. In the writhing method at a dose of 2.6mg/kg and 5.2 mg/kg in albino mice vilazodone showed 30.33±1.15 and 28.16±0.98 analgesic activity compared to control. In formalin induced peritonitis vilazodone 1.8mg/kg and 3.6mg/kg showed 2.2±0.13 and 1.86±0.05 anti-inflammatory activity when compared...
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Posted by admin on Apr 30, 2023 in |
Simple, very Sensitive, rapid, and accurate novel spectro-photometric methods were developed to estimate Lenvatinib in Pharmaceutical dosage form using zero- and first-order derivative spectrophotometry. The solutions of Lenvatinib standard and sample were prepared using analytical grade methanol. The spectrophotometric estimation of lenvatinib was carried out at 240 nm in the zero-order method and 253 nm in the first-order derivative method. Developed methods were validated with respect to linearity, precision, accuracy, limit of detection and quantitation in accordance with International Council for Harmonisation (ICH) guidelines. Linearity was observed in the concentration range of 1-5 µg/mL for zero- and first-order derivative methods. LOD and LOQ of Lenvatinib were found to be 0.017 µg/mL and 0.052 µg/mL for zero-order and 0.165 µg/mL and 0.500 µg/mL for first-order derivative spectrophotometric methods, respectively. The %RSD values for inter-day and intra-day precision study were <2.0%. The method was linear, accurate and precise with recoveries in the range of 98 – 102 %, and minimum values of %RSD indicate the method’s accuracy. Developed spectrophotometric methods were...
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Posted by admin on Apr 30, 2023 in |
Allopathic drugs are generally prescribed for Diabetes Mellitus (Type-II) patients. As the disease is chronic in nature, it necessitates the use of alternative medicine(s) either to supplement the existing allopathic therapy and/or to reduce the associated side effects with their chronic use. The use of herbal drugs is presumed to be safe option due to fewer side effects. However, evaluation of safety of their combination by scientific validation is very essential. Here we evaluated the safety of routinely used second generation drug Gliclazide along with Berberine (herbal drug). The study was conducted in two dissimilar species, rats, and rabbits. In rats and rabbits, the dose of 100 mg/kg of Berberine and 1 mg/Kg Gliclazide dosed separately produced~30% reduction in blood glucose. When both Berberine and Gliclazide were given in combination at similar doses, reduction in blood glucose was found to be ~60%, suggesting significant pharmacodynamic interaction between them. Also, in the rabbit study, Gliclazide pharmacokinetic parameters were studied. We observed significant changes in the pharmacokinetic parameters of Gliclazide (AUC,...
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