Posted by admin on Nov 1, 2010 in |
Methanolic extracts of the tuber of Amorphophallus paeoniifolius were investigated for its antihelmintic activity against Pheretima posthuma and Tubifex tubifex. The extract with the concentrations of 25, 50 and 100 mg/ml were tested in the bioassay, which involved determination of time of paralysis and time of death of the worms. The extract exhibited significant antihelmintic activity at highest concentration of 100 mg/ml. Piperazine citrate (10 mg/ml) was included as standard reference and distilled water as control. The extracts were found not only to paralyze (Vermifuge) but also to kill the earthworms...
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Posted by admin on Nov 1, 2010 in |
Cleome rutidosperma is traditionally used in the treatment of paralysis, epilepsy, convulsions, spasm, pain and skin disease. Extraction of the aerial parts of the plant Cleome rutidosperma using water and ethanol and evaluation of their antioxidant activity has been envisaged in this present research work. Both the extracts exhibits significant antioxidant activity in DPPH, Nitric oxide and hydroxyl radical induced In-vitro assay methods. The DPPH radical inhibition (%) was 53.13, 57.13 and 79.12 for CRA, CRE and ascorbic acid respectively. The Cleome rutidosperma extracts (CRA and CRE) showed significant free radical scavenging action against nitric oxide (NO) induced release of free radicals at the concentrations 250μg/ml, showing 29.22% and 63.32% of NO inhibition, respectively. The CRA and CRE extracts (25-400μg/ml) significantly scavenged the hydroxyl radical generated by the EDTA/H2O2 system, when compared to that of ascorbic...
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Posted by admin on Nov 1, 2010 in |
A simple, precise, accurate and rapid high performance thin layer chromatographic method has been developed and validated for the simultaneous estimation of Lamivudine and Abacavir sulphate in combined dosage forms. The stationary phase was precoated silica gel 60F254. The mobile phase used was a mixture of (Acetone: chloroform: methanol 4: 4: 2 v/v/v). The detection of spot was carried out at 265nm. The method was validated in terms of linearity, accuracy, precision and specificity. The calibration curve was found to be linear between 500 to 3000 ng with regression coefficient of 0.9998. The proposed method can be successfully used to determine the drug content of marketed...
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Posted by admin on Nov 1, 2010 in |
A high performance liquid chromatography method was developed for the simultaneous separation and quantization of Atorvastatin calcium and Amlodipine besilate in combined pharmaceutical tablet dosage form. The chromatographic estimation was achieved by using mobile phase consisting of a mixture of phosphate buffer (1ml ortho phosphoric acid in 1000 ml 0f water) acetonitrile and methanol (53:43:4, v/v) at a flow rate of 1 ml/min and UV detection at 246 nm, using photodiode array detector. The determination was carried out by using Grace Smart RP C-18 column (250 X 4.6 mm, 5 µm). The retention time of Amlodipine besilate and Atorvastatin calcium was 3.337 and 6.067 respectively. Amlodipine besilate and Atorvastatin calcium calibration curves were linear with correlation coefficient 0.9989 and 0.9981 over a concentration range of 40-60 µg/ml and 80-120 µg/ml respectively. Recovery was between 99.60-100.02 for AML and 99.05-100.52% for ATV,...
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Posted by admin on Nov 1, 2010 in |
Diabetes induced neuropathic pain is recognized as one of the most difficult type of pain to treat and conventional analgesics are well known to be partially effective or ineffective. Synthetic or natural cannabinoids are promising drugs to treat neuropathic pain. It has been reported that the effect of cannabinoid analgesics is not compromised in various experimental neuropathic pain model. The aim of present study was to explore the ameliorative potential of cannabis sativa extract in hyperglycemia-induced neuropathic pain. Streptozotocin (STZ) (20 mg/kg, i.p. x 4 days) was administered to induce experimental diabetes in the rats. The pain threshold in diabetic and non-diabetic rats was measured using paw withdrawal latency test. After 28 days of STZ injection, diabetic rats exhibited a significant thermal hyperalgesia and mechanical allodynia along with increased plasma glucose and decreased body weights as compared with controls rats. Moreover, STZ administration was noted to increased oxidative stress (MDA & nitrite level) and decreased antioxidant enzyme levels (glutathione, catalase and superoxide dismutase) significantly in diabetic rats as compared...
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