Posted by admin on Jun 30, 2023 in |
Heavy metal pollutants in tannery effluent can be removed using the bioremediation technique. This study intends to extract bacteria that may reduce heavy metals and molecularly characterize them using 16S rRNA PCR in samples taken from tannery effluent. It also studies their cell-free immobilized enzymatic activity. Thirteen bacterial isolates were identified based on morphological, cultural, and biochemical characteristics. The top four isolates, A, B, sp5, and sp3, were identified as Brevundimonas sp, Proteus mirabilis, and Alcaligenes faecalis by morphological, cultural, biochemical, MALDI-TOF Analysis, and molecular characteristics using 16S rRNA. These organisms showed good tolerance to zinc, nickel, and chromium. When heavy metals like zinc and chromium have reduction properties, by ICP-OES to assess the concentration of heavy metals. Further investigation, beads were immobilized, and its activity was...
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Posted by admin on Jun 30, 2023 in |
Background: Children with mild intellectual disabilities demonstrate wide diversity in academic and behavioural performance. They develop adequate social, personal, and communication skills. As adults, they function in a variety of contexts. Persons with moderate intellectual disabilities have limited intellectual abilities, have problems in abstracting and generalizing, and are not ready for much academic work until their early teens. They show rather clear-cut deficits in adaptive behaviour. The children with mild intellectual disabled show underachievement in all academic areas, have difficulty in working with abstract ideas, and have problems generalizingtheir learning to new situations. The study aims to explore the effect of social skills on the problem behaviour and academic performance of children with mild intellectual disability in aninclusive setting. Materials and Methods: 400 Children with intellectual disability and their teachers in the inclusive set-up both in rural and urban areas of Trichy District were chosen for the study. The data was collected through the Social Skills Rating System (SSRS)-Gresham & Elliott, 1990). Obtained data were transcribed and analyzed using Descriptive and Differential...
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Posted by admin on May 31, 2023 in |
This study aimed at identifying promising breast cancer inhibitors through in-vitro and in-silico studies. Ocimum basilicum. L is a traditional herb used for varied ailments. The present study is designed to evaluate the anti-carcinogenic potency of Palustric acid and Andrographolide against the MCF-7 cell line by in-vitro analysis. Further in-silico examination was performed to detect and formulate Receptor-ligand complex of estrogen using molecular docking technique. In-vitro study was conducted using MTT assay and microscopic examinations to determine the cell viability and morphological changes in MCF-7 cells. In-silico, scrutiny was performed using virtual screening, Docking, ADME, and molecular dynamic simulation to evaluate the stability of the compounds. Palustric acid and Andrographolide against the MCF-7 cell line by in-vitro analysis. Further in-silico examination was performed and showed an outstanding anti-cancer potential, with dose- and time-dependent patterns in MTT assay and through the fluctuating organization of MCF-7 cells. In-silico analysis showed that the selected lead compound-complex exhibited good stability and was a highly potent inhibitor against the target breast cancer receptor. This...
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Posted by admin on May 31, 2023 in |
In the present study, biosurfactant-producing microorganisms such as KM1 (Bacillus licheniformis – Kasemedu 1), KM2 (Bacillus subtilis – Kasemedu 2), ER1 (Pseudomonas aeruginosa – Ennore site 1), ER1B (Pseudomonas mendocina – Ennore site 1) and ER9 (Streptococcus lactis – Ennore site 2) were subjected to optimization studies. The optimization study was carried out using various carbon, nitrogen sources, pH, temperature, hydrocarbons, and trace elements. The effect of dry cell biomass, biosurfactant production, emulsification activity, and surface tension were studied. Among the five isolates, KM2 and ER1 grew well in sucrose at 1% concentration. Production and activity of the biosurfactant were found to a maximum at pH 7.0, and an additional increase in pH during bacterial growth does not affect the surfactant activity till pH 8.5±0.2; however, a decrease in pH showed a significant effect on the surfactant activity. The optimum temperature was identified as 37°C for all the isolates and below and above this temperature markedly affects the biosurfactant production and activity. Observations on reduction in surfactant activity with...
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Posted by admin on May 31, 2023 in |
The relevance of traditional medicines and their folkloric applications for the well-being of humanity have been highlighted in the current study. To generate the polyherbal extract for the in-vitro assessment of anticancer activity, the researcher has investigated the standardization criteria for the individual plant drug and extract obtained from these herbs. According to the literature and earlier studies, all four of the herbs utilized to create polyherbal extract have the potential to cure a variety of malignancies in addition to liver carcinoma. The physiochemical and pharmacological value of each medicinal herb and the polyherbal extract of these four plant drugs (Vinca, Jackfruit, Lawsonia, and Ashwagandha) are revealed in the same research findings. Based on the results of phytochemical testing, it is evident that the majority of these phytochemicals employed include the alkaloids and steroids in n-hexane extract, which also suggests the possible anticancer effects of each drug separately. The MTT dye assay assessed the polyherbal extract’s cytotoxic impact on the Huh7 cell line. The polyherbal extract’s anticancer results are...
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