Posted by admin on Jul 1, 2010 in |
A novel method was developed for the determination of fluoroquinolones such as ciprofloxacin hydrochloride (CPL) and ofloxacin (OFX) by isocratic reverse phase-high performance liquid chromatography (RP-HPLC) coupled with UV detection. The developed method was rapid, accurate, reproducible, economical and sensitive for the simultaneous estimation of ciprofloxacin hydrochloride and ofloxacin. Fluoroquinolone antibiotics were separated on an analytical column (SIL 100Å, 125×4.6 mm ID) C18-RP, 5 µm, at ambient temperature. The mobile phase was consisted of Phosphate Buffer (15 mM): Methanol: Acetonitrile: TEA (66:24:10:1% v/v), pH was 6.5 (adjusted with 80% HCl), at a flow rate 1ml/min and sample injection volume was 20-µL. UV detection at 289 nm and retention time (RT) was 3.01 and 6.03min for ciprofloxacin hydrochloride and ofloxacin respectively. The method was validated in terms of stabilizing accuracy, precision (Intra/Inter-day), linearity, specificity, stability and sensitivity. The proposed method has been successfully applied for the analysis of marketed tablets and can be used for the routine analysis of formulations containing any one of the above drugs without any alteration...
Read More
Posted by admin on Jul 1, 2010 in |
The effect of Adenanthera pavonina (AP) seed extract on the blood cholesterol level of atherogenic diet rats was evaluated. Twelve adult male Wistar rats divided into 3 groups of 4 animals each were used and were treated orally with normal saline (Control group), atherogenic diet (Positive control) and 200mg/kg of AP seed extract (treatment) over a 4-week period. The present study was undertaken to assess body weight, total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL) and very low density lipoprotein (VLDL). They were significantly lower in the extract treated groups compared to the control group. Phytochemical screening showed that the extract contained cardiac glycosides, tannins, saponins, alkaloids and flavonoids. Cyanogentic glycosides and anthraquinones were absent. Histopathological examination showed that the extract did not cause any significant lesion changes in the liver, kidney and even the testes. The study showed that Adenanthera pavonina (AP) seed extract have the potential to cause a blood cholesterol lowering effect. The serum biochemistry changes may suggest that the extract...
Read More
Posted by admin on Jul 1, 2010 in |
The genus Mangifera indica belongs to the family Anacardiaceae and comprises of 40 species distributed in tropical and sub-tropical parts of South East Asia, Africa and Latin America The resin is useful in cutaneous disease .the juice of kernel is snuffed to stop nasal bleeding. A decoction of the leaves with a little honey added is given in aphonia, the loss of voice. Mid ribs of the leaves calcined are used to remove warts on eyelids. Tender leaves, dried and made into a powder, are useful in diabetics. Smoke of the leaves is said to have a curative effect in some infections of throat diseases. Ashes of leaves are a popular remedy for burns and scalds. Dried flowers in decoction powder and useful in diarrhea, chronic dysentery and gleets .Powder of flowers are used for fumigation against mosquitoes. Gum resin of the tree is applied with benefit to cracked feet. Gum resin from the bark is used in catarrhs and when mixed with lime juice and applied, it is...
Read More
Posted by admin on Jul 1, 2010 in |
Withania somnifera Dunal. (ashwagandha) is widely used in Ayurvedic medicine, the traditional medicinal system of India. It is an ingredient in many formulations prescribed for a variety of musculoskeletal conditions (e.g., arthritis, rheumatism), and as a general tonic to increase energy, improve overall health and longevity, and prevent disease in athletes, the elderly, and during pregnancy. In the recent past tremendous attempts are being made to evaluate the scientific standards for Traditional system of medicine. For the standardization of this drug Pharmacological and Phytochemical parameters were carried out such as moisture content, ash values, pH values, extractability in water and alcohol were carried out. Thin Layer Chromatography studies were also carried out to ascertain the quality of this...
Read More
Posted by admin on Jul 1, 2010 in |
Antioxidant and phytochemical properties of an ethanolic extracts from the wild edible mushroom Termitomyces reticulatus and their individual parts (Cap and Stipe) were evaluated. Reducing power, β-carotene bleaching, ABTS and DPPH radicals scavenging activity were used to evaluate their antioxidant activity. Antioxidant components like total phenol, flavonoid, β-carotene and lycopene were also determined and the amount of phenol was correlated with the antioxidant property. All the extracts showed potent antioxidant activities, in which the entire mushroom extract showed more antioxidant property when compared with the other two extracts (Cap and Stipe) and they were in the order, entire mushroom>stipe>cap. Total phenol exhibited a negative correlation with the EC50 values of antioxidant assays in all the extracts. The phytochemical profile of the ethanolic extracts, analysed were found to contain alkaloids, saponins, phenols, anthraquinones, steroids, carbohydrates, proteins and cardiac glycosides. The tannins and toxic content cynogenic glycosides were absent. In conclusion, our results supported the consumption of wild edible mushroom that act as a good dietary supplement, taken as whole or...
Read More
