Posted by admin on May 31, 2023 in |
Healthy diet has been known to play a key role in managing and preventing diseases. The health benefits of consuming common beans (Phaseolus vulgaris) are widely acknowledged and are ascribed mostly to their high dietary fiber contents and polyphenols. Various animal experimental studies have shown that polyphenol in common beans has antioxidant properties and other biological activities. This study was designed to evaluate the antilipidemic potentials of Phaseolus vulgaris on experimentally-induced diabetic rats. 20 male rats of the Wistar strain were divided into 4 groups (n=5). Group A was the normal control; group B was the diabetic control; while groups C and D were diabetic and treated with 400mg and 800mg/kg body weight of aqueous extract of Phaseolus vulgaris seed, respectively. Blood samples were collected on the last day of the experiment for biochemical assay. Results showed a significant increase (p<0.05) in total cholesterol (TC), triglyceride (TG), and low-density lipoprotein (LDL-c) levels of diabetic rats when compared to the control group. Treatment of diabetic rats with Phaseolus vulgaris showed...
Read More
Posted by admin on May 31, 2023 in |
Solubility and stability are the limiting steps for almost all drugs in the pharmaceutical field toward bioavailability. In the present investigation, microwave reacts with the crystallization solvent to change their crystalline state to stable and highly soluble nanocrystals. Microwave Assisted Grinding Nanotechnology (MAGN) was used to prepare stable nanocrystals of Valsartan, BCS class II, by treating them with Chitosan and neusiline US2. Ternary crystalline particles were designed using 32 level full factorial design using Design Expert Software version 12. The optimized batch showed a nano-size range with about a 3.2 times increase in saturation solubility and 2.89 times enhanced cumulative drug release than pure valsartan. Compatibility with excipients, crystallinity and particle size study was confirmed from FTIR, DSC, XRD, and particle analyzer. Physical interactions from FTIR and NMR results also confirmed intermolecular hydrogen bond interaction. The accelerated stability study showed a confirmed stable product due to the absence in any interactions from FTIR and variation of drug content. Formulation of microwave-treated nanocrystals is a novel technique to enhance bioavailability...
Read More
Posted by admin on May 31, 2023 in |
Pyrazole and its derivatives are pharmacologically active and are being used in various disorders and diseases with a few side effects. The wide distribution of pyrazole compounds in nature with fascinating pharmacological action is key in the application of this development of newer derivatives. The main structure has a great variety of compounds that have essential pharmaceutical and agrochemical activities in addition to biological activity like anti-inflammatory, antifungal, antitumor, antiviral, antibacterial, anti-tubercular, and antiphrastic. Protection against various substances that causes oxidative stress in the body is needed in the modernizing world in this direction intermediate obtained from the Vilsmeier Haack reaction (4) was treated with aromatic amines to obtain N-[3-(naphthalen-1-yl)-1-phenyl-1H-pyrazol-4-yl] methylene) derivatives (5a-5l). The in-vitro antioxidant activity of newly synthesized compounds was determined by the Nitric oxide scavenging...
Read More
Posted by admin on May 31, 2023 in |
A Quality design approach to method development involves method goal identification, method scouting and evaluation, method selection, and risk assessment. The present study describes the risk-based HPLC method development and validation of Formoterol fumarate and Aclidinium bromide in a pharmaceutical dosage form. The chromatographic conditions were optimized with the Design Expert software 11.0 version i.e., Kromasil C18 (250×4.6 mm, 5 µm) Mobile phase used was 0.01N Kh2po4 (pH :3.0) (52.4%): Acetonitrile (47.6%), flow rate was found to be 1.0ml/min with retention times of formoterol 2.456 min and Aclidinium 3.573min. The developed method was found to be linear in the range of Formoterol 1.5-9μg/ml, aclidinium 50-300μg/ml with a correlation coefficient of 0.999 for both drugs. The % RSD of intraday and inter-day precision for Formoterol 0.3 & 1.0, Aclidinium 0.7 & 0.4. The robustness values were less than 2%.The assay was found to be 99.91%&99.99% for formoterol and aclidinium bromide. The method validation parameters were in the prescribed limit as per ICH guidelines. Stress studies reveal that both drugs were...
Read More
Posted by admin on May 31, 2023 in |
Antimicrobial resistance is a threat to public health. This study investigated the occurrence of ESBL and colistin resistance among Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa recovered from clinical samples (n=500) in a tertiary teaching hospital in Abakaliki, Nigeria. The samples were bacteriologically analyzed using selective culture media, biochemical tests, and PCR. All isolates were screened for ESBL and colistin resistance genes using both PCR and disk diffusion methods that included standard antibiotic disks for susceptibility studies. E. coli (n=173), K. pneumoniae (n=120) and P. aeruginosa (n=62) were recovered from the clinical samples investigated. The most prevalent ESBL genes detected is blaCTX-M found in 3 isolates of P. aeruginosa, 4 isolates of E. coli and 2 isolates of K. pneumoniae. The colistin resistance gene, mcr-3 was detected in 4 strains of E. coli for the first time in Southeast Nigeria. Both mcr-1 and mcr-3 was not detected in the K. pneumoniae strains. Only the mcr-2 colistin resistance genes were detected in K. pneumoniae strains. There was no detection of...
Read More
