Posted by admin on Mar 31, 2023 in |
Misuse and overuse of antibiotics, multi drug resistance bacterial strains have emerged as silent pandemic. Lately, antibiofilm treatment approach using natural compounds have ascended as an alternate strategy to overcome the issue. In this study, the inhibitory effect of bark and leaf extracts of Acacia ferruginea against a model bacterium, Chromobacterium violaceum was evaluated. Results revealed that A. ferruginea extract has a significant inhibitory effect on virulence determinants (biofilm and violacein productions) with no effect on growth.19 unrelated compounds were identified from GC-MS analysis. In molecular docking studies, six hits were found to have potential interactions with CviR, a quorum sensing regulatory protein. One of the major hits, severin, formed H-bond with Ser155 and Asp 97 with highest Gscore -8.862 when compared to the standard ligand, C6HSL (-7.052). Our data showed that the leaf and bark ethanolic extracts of A. ferruginea will be a promising synergetic remedy against bacterial infections from natural sources by curbing quorum...
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Posted by admin on Mar 31, 2023 in |
Peripartum cardiomyopathy (PPCM), a rare condition affecting women in late pregnancy and early puerperium, has high morbidity and mortality. It is characterized by impaired contractility with a low ejection fraction. Compensation occurs through the enlargement of the left ventricle with increasing end-diastolic and stroke volumes. As the compensation of the dilated ventricle fails, it leads to systolic dysfunction, progressing to congestive cardiac failure. Early recognition and aggressive management of these cases in the perioperative period is crucial to improving outcomes of both mother and child. The major anesthetic concern in managing these patients is to avoid myocardial depression and optimize cardiac output, thus maintaining myocardial perfusion with stable intraoperative hemodynamics. Neuraxial anesthesia in these cases, must be balanced with their hemodynamic effects and the ability of the patients to compensate for the physiological strains. Epidural Volume Extension (EVE) – saline technique offers the rapidity and reliability of spinal anesthesia and the flexibility of epidural anesthesia. We present a case series of five patients posted for emergency cesarean section who...
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Posted by admin on Mar 31, 2023 in |
The present work was executed to evaluate the anti-diabetic potency of a polyherbal preparation. The objective of this study is to induce experimental diabetes mellitus using Streptozocin -nicotinamide in normal Albino wistar rats and study the antidiabetic activity of polyherbal formulation by comparison of changes in body weight and levels of glucose between normal and diabetic rats. Hypoglycemic agents from natural and synthetic sources are available for treatment of diabetes. Indian medicinal plants have been found to be useful to successfully manage diabetes. The effect of alcoholic extract of poly herbal preparation containing leaves of Gymnema sylvestre, fruits of Momordica charantia, rhizomes of Curcuma longa, seeds of Eugenia jambolana and fruits of Embilica officinalis was investigated in normal, glucose load conditions and streptozocin -nicotinamide induced diabetic rats. Significant anti diabetic activity was exhibited by the poly herbal formulation. Treatment with the polyherbal Preparation 200 mg/kg body wt and 400 mg/kg body wt for 10 days in diabetic animals has shown significant decrease in serum glucose levels in comparison to...
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Posted by admin on Mar 31, 2023 in |
Different-different modification of Benz-oxazole moiety gives various numbers of novel compounds which shows various biological activities. Some new derivatives (G7-G10) containing Benzoxazole moiety were prepared by the use of different methods and reagents. O- Phenylenediamine treated with Chloroacetic acid to form 2(chloro methyl) benzimidazole (compound B) and 4- methyl, 2- amino phenol with methyl alcohol and carbon di sulfide to form 2-thio, 5- methyl, benz-oxazole (compound G1). Further Compound B treated with (compound G1) to form [(benz-imidazole)-2-methyl thio]-5-methyl, benzoxazole (compound G2). Finally compound G2 treated with a substituted aldehyde to form benzoxazole derivatives (G7-G10). Compound spectral analysis was done by Infrared spectroscopy (IR), 1H-Nuclear magnetic resonance (1HNMR) & Liquid chromatography-mass spectroscopy (LCMS). The serial Dilution method (MIC test) was used for anti-bacterial activity, and Alamar blue assay test was used for Anti-tubercular activity. Compounds G8 and G9 showed potent anti-tubercular activity against M. Tuberculosis, and Compounds G7, G8 and G10 were showed good antimicrobial...
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Posted by admin on Mar 31, 2023 in |
Adrenergic receptors (ADRs) are implemented in important pharmacological responses. The β3adrenergic receptors have been proposed as a suitable drug target for treating diabetes and obesity. However, very little information is available regarding the expression profile of β3 receptors. We have designed primers for studying the β3adrenergic receptors in C57BL/6J mice using various bioinformatics tools in the present study. The primers were designed using three different software, primer 3 Plus, NCBI Primer BLAST, and Primer express, and validated using UCSC in-silico PCR and NCBI -Primer-BLAST. The best primer pair was selected based on primer melting temperature, GC% and primer length. The selected primer pair was custom synthesized. The primer annealing temperature was standardised using gradient PCR. Among the three software used for primer designing, primer express was found to be the best, and the primers designed from primer express were found to have the ideal melting temperature between 58-60°C, 50% of GC content, and 20bp length. From gradient PCR, the primer annealing temperature was found to be 58.3°C. The bioinformatics tool is...
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