Posted by admin on Feb 28, 2023 in |
Frying is one of the oldest food processing methods. Its popularity is related to the ease and speed of food preparation and sensory characteristics, such as unique flavor and taste. Nowadays, the consumption of deep-fried food has gained popularity which causes an increase risk of different disease conditions such as obesity, cardiovascular disease, diabetes, hypertension, etc. So, the validation and standardization of fried foods commonly used in the household were more important. In this study, the Physiochemical and Organoleptic changes in repeatedly heated different types of oil such as sesame oil, coconut oil, groundnut oil, and refined Sunflower oil for preparing homemade popular South Indian snacks (Muruku) were studied. Density was found to increase gradually in peanut oil and refined sunflower oil. Viscosity was found to be stable in sesame oil. While the viscosity reduces in coconut oil, it increases in refined sunflower oil. The refractive index of the fresh and fried oil was found to be close enough to all the oils except refined sunflower oil, which had...
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Posted by admin on Feb 28, 2023 in |
To study reverse aging in Saccharomyces cerevisiae (S. cerevisiae) by gene expression analysis of target of rapamycin (TOR1) induced autophagy and replicative life span modelling (RLS) using Alpinia galangal (A. galangal), Commiphora mukul (C. mukul), Acorus calamus (A. calamus) and Morshella esculenta (M. esculenta). Methanolic extraction of plants was carried out using maceration. Activation of yeast was performed using the sugar fermentation method. Pharmacological anti-aging screens in yeast performed by RLS. Primers for Actin (ACT1) and TOR1 were designed using Primer-BLAST. Gene expression analysis (RNA isolation and quantification, reverse transcription, and real-time polymerase chain reaction) of TOR1 in S. cerevisiae was conducted using methanolic extracts. Phenolic and Flavonoid compounds were quantified using reversed-phase high-performance liquid chromatography (RP-HPLC). Cell counting and viability assessment of S. cerevisiae with trypan blueusing methanolic extracts was performed. Gene expression analysis results revealed under expression of TOR1 in S. cerevisiae with mean normalized expression ratios < 1 using all four methanolic extracts. Results from RLS showed increased absorbances due to decreased TOR1 activity. HPLC analysis...
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Posted by admin on Feb 28, 2023 in |
Rheumatoid arthritis is a systemic autoimmune and inflammatory disease that primarily affects the synovial joints and eventually destroys them. Schleichera oleosa is a plant of Indian provenance that exhibits anti-rheumatoid arthritis activity. The phytochemical constituents in the methanol extract of barks from Schleichera oleosa were identified using gas chromatography-mass spectrometry (GC-MS) in the current study. The GC–MS analysis in bark extract identified 21 phytochemical compounds. The PASS server assessed the biological activity of the reported compounds and identified six compounds with anti-arthritic and anti-inflammatory activities. Subsequently, these compounds were screened through ADME/Tox and molecular docking approaches. Two compounds 2,4-Di-tert-butylphenol and 2,6-DI-Tert-butylphenol qualify for all the criteria of ADME/Tox. The molecular docking method identified compounds 2,4-Di-tert-butylphenol and 2,6-DI-Tert-butylphenol had binding affinity ranging between -4.89 to -6.49 kcal/mol with the active site of anti-arthritic target proteins Chitotriosidase-1 and Vascular cell adhesion molecule 1. The findings of this study will create a way to invent herbal medicines for Rheumatoid Arthritis using the Schleichera oleosa plant, potentially leading to the development of new...
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Posted by admin on Feb 28, 2023 in |
Nabumetone (NBT), a non-steroidal anti-inflammatory drug used to treat rheumatoid arthritis, is rapidly metabolized in the liver and is well known for its side effects along with its sensitivity to light; thus, it limits the formulation of a such drug when considered for skin formulations. Recently introduced a new type of carrier for bioavailability benefits and patient compliance, the “transfersomes”. Due to its elastic nature, which can deform and squeeze themselves because of their flexibility through narrow pores of stratum corneum, the transfersomes were prepared by use of phospholipid and sodium cholate, tween 80 as edge activators. The NBT loaded transfersomes were optimized by the three factors and two levels Box-Behnken design using Design-Expert software (version 12). Independent formulation variables such as concentrations of a phospholipid, concentration of sodium cholate, and tween 80 were evaluated. The prepared TFs were evaluated with respect to particle size, % entrapment efficiency, and % drug release. The optimized batch F1 was formulated by incorporation into a Carbopol-940 gel base. The prepared NBT-loaded TFs...
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Posted by admin on Feb 28, 2023 in |
Pain is the most common reason for physician consultation. Among the currently available analgesic drugs, opioids are the most efficacious but have many unacceptable adverse effects. Melatonin has been found to have antinociceptive potential in many animal and clinical studies that need further evaluation. The study aimed to assess the antinociceptive effect of melatonin alone and in combination with pethidine. Twenty Swiss albino mice were divided into four groups, each containing five animals receiving respective drugs. Tail flick and tail clip methods were used to assess antinociceptive effect of the drug. The reaction time of each animal in each group was taken at 0 min, 30 min, 60 min, 90 min and 120 min and the experiment was conducted on day 0, day 7, day 14 and day 28. Mean reaction time averaged over day 0, day 7, day 14 and day 28 was used to make inter-group comparisons. The mean reaction time increased in all groups except group I, in both tail flick and tail clip methods suggesting...
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