Posted by admin on Apr 30, 2023 in |
For the present study, 10 bacterial strains were isolated from agriculture field soils collected from Guntur, Andhra Pradesh, India, and thereafter screened for amylase activity. Among them, the strain DS5 showed a maximum zone of clearance on the starch hydrolysis test was selected for this study. Identification of bacterial strain DS5 was confirmed by 16S rRNA sequencing analysis. The phylogenetic tree constructed on the basis of 16S rRNA sequences revealed that it clustered with the closest members of Bacillus pumilus DS5 and the sequences were deposited in NCBI Gen bank along with accession number MK430437. Further the effect of incubation period, temperature, pH, carbon and nitrogen sources was optimized. Highest amylase production was found after 48 h of incubation at pH 7.0 and 40 ⁰C temperature. Starch proved to be the best carbon source and peptone as nitrogen source for the highest production of amylase by Bacillus pumilus DS5. Supplementation with different nitrogen and carbon source favoured the increased enzyme yield by submerged...
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Posted by admin on Apr 30, 2023 in |
Diabetes mellitus is a complex disorder where oxidative stress plays a crucial role in the development and progression of the disease. In the present work, the rat model evaluated pancreas and liver tissue damage caused by a high-fat diet and diabetic complications. Male Wistar rats were administered with HFD- corn oil orally for 28 days followed by streptozotocin (STZ) injection to induce diabetes on 29th day. The drug treatment groups were given low dose (250mg/kg) and high dose (500mg/kg) of hydroalcoholic extract of Achyranthes aspera (HEAA), and its therapeutic efficacy in HFD + diabetes model was compared with the standard drug metformin. Various antioxidant parameters were measured, including MDA, protein, GSH levels, SOD, and CAT activities. The histology of pancreas tissues was also analyzed using Haematoxylin, Eosin, and Masson-trichrome staining. The expression of insulin protein in the pancreas was studied by immunohistochemical technique. The effect of drugs on GLUT-2 and PPAR-α gene expression showed considerable improvement in the liver tissues of different groups. From the results, it can be...
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Posted by admin on Apr 30, 2023 in |
Background: Our study aims to document the use of ethnomedicinal plants reported by herbalists in treating ailments that are becoming more prevalent. Using an ethnobotanical approach and in-silico studies, will advise further prevalence and give precedence to future pharmacological studies. Methods: The application of the plants was reported by 75 informants. From the 51 reported plants, 20 plants were frequently cited as having high potential anti viral and anti-inflammatory activity and were validated in in-silico approach. PASS was used for compound prediction, and AMT was calculated with the help of ADMET lab 2.0. Results: Out of the 20 plants validated in in-silico, the results of reported compounds revealed that Aeginetia indica could best act as an antiviral agent with Pa (0.939) and Pi (0.003), followed by Bacopa monnieri with Pa (0.880) and Pi (0.002) and Blumea balsamifera with Pa (0.743) and Pi (0.004). Subsequently, the plant which can act as the best anti-inflammatory ailment is Andrographis paniculata with Pa (0.845) and Pi (0.005), followed by Pisum sativum with Pa...
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Posted by admin on Apr 30, 2023 in |
Vigna aconitifolia L. represents a genus in the Fabaceae family and is one of the most drought-resistant legumes, commonly grown in arid and semi-arid regions of India. Therapeutic effects of Vigna aconitifolia L. have been reported in several publications, but there are only a few reports on the biological activities of the Vigna aconitifolia L. proteins. In the present study, antibacterial activities of the Vigna aconitifolia L., peptides have been investigated. Homogenized seed flour was pulverized, and its protein content was extracted in a phosphate buffer. After centrifugation, by adjusting pH, peptides from the supernatant were isolated. Gel filtration (G-25) and Ion-exchange chromatography on the seed extract gave fairly pure peptide fractions of which inhibitory activities were done by agar well diffusion method against Shigella flexineri, Bacillus cereus, Salmonella typhimurium, Staphylococcus aureus, Enterococcus faecalis, Vibrio cholerae bacterial pathogens. The results indicate that a 30kDa of the peptide was identified and exhibited good antibacterial activity against bacterial pathogens. Among all strains, Shigella flexineri and Staphylococcus aureus exhibit good results with a clear zone of...
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Posted by admin on Apr 30, 2023 in |
The objectives of the present study were to determine the total phenolic content, flavonoid content, and anti-cataleptic activity of chloroform and methanolic extract of the roots of Quisqualis indica. The crude extracts were investigated for total phenolic and flavonoid content using the Folin-Coicalteu method and the Aluminum chloride colorimetric method. It was found that the total phenolic content is greater than the flavonoid content in Q. indica roots. The total phenolic content of a methanolic extract is greater than the chloroform extract as 61.83 μg/ml and 25.40μg/ml, respectively. The flavonoid content in methanolic and chloroform extract was found to be 10.22μg/ml and 2.11μg/ml. The anti-cataleptic activity of the Q. indica roots extracts was evaluated by Wood block test. Catalepsy was induced by clonidine 1 mg/kg, subcutaneously and Chlorpheniramine maleate 10 mg/kg i.p as the standard drug. The vehicle-treated group has shown maximum duration of catalepsy (190.33±3.68) at 180 minute after the administration of clonidine. There was significant inhibition of clonidine induced catalepsy in the animal pre-treated with methanol and...
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