Posted by admin on Jan 31, 2023 in |
Introduction: Alcohol de-addiction is challenging due to the severe alcohol withdrawal symptoms. This article reports ameliorative effect of KalyanakGhrita (KG) on ethanol withdrawal symptoms in animal model. Material and Methods: After approval from Animal Ethics committee, alcohol dependence was developed in pre Rota road trained Swiss mice by intermitted access of 20% ethanol in 14 days. After confirmation of dependence, the animals were allocated in 6 groups, 6 animals in each group. Group I &II received distilled water & ethanol respectively. Group III to VI received cow ghee, KG in two dose level and Naltrexone respectively along with ethanol till next 14 days. Further alcohol abstinence was given but treatment drugs were continued for next 7 days. To note the effect of KG on withdrawal symptoms, animals was introduced to Rota rod on 0 day and 3rd day for muscular incoordination and to EPM on day 0 day & 7th day after abstinence of ethanol for anxiety assessment. Results: KG higher and lower dose demonstrated significant reduction in anxiety...
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Posted by admin on Jan 31, 2023 in |
The Hemagglutinin (HA) protein of the Influenza virus plays a vital role in viral replication. In this study, numerous small molecules were screened systematically against the HA protein to find a compound that can bind to it selectively. The HA protein’s three-dimensional crystal structure was obtained from RCSB PDB and inputted into the FTMap Web Tool to identify the best possible drug gable hotspot. Over 68 million compounds from the ZINC-15 database were filtered for drug-likeness properties and narrowed down to 250,000 ligands. These chosen molecules were then tested for their binding affinity to the selected hotspot on the HA protein using the CLC Drug Discovery Software. The top 100 molecules with the highest binding affinity were tested for ADMET properties using the admet SAR web tool. Six compounds that satisfied the given conditions were then subjected to a more intensive binding affinity test using the AutoDock Vina program of the PyRx software. The molecule with the highest binding affinity to HA, 6-(4-Methylpiperidin-1-yl) sulfonyl-1-azatricyclo [6.3.1.04, 12]dodeca-4,6,8(12)-trien-2-one, was selected as...
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Posted by admin on Jan 31, 2023 in |
The present study aims to evaluate the in-vitro antioxidant, in-vitro α-amylase inhibition, in-vitro and in-vivo anti-cataract potential of ethyl acetate root extract of Abutilon indicum. Antidiabetic activity was evaluated against alpha-amylase by spectrophotometric assays. DPPH determined antioxidant activity, Reducing power assay, Phosphomolybdenum antioxidant assay, Nitric oxide-scavenging activity, Hydrogen peroxide scavenging, and Hydroxyl radical scavenging assay. Different extract concentrations were made using dimethyl sulfoxide (DMSO) and subjected to an α-amylase inhibitory assay. The in-vitro anticataract potential of Abutilon indicum was determined using glucose-induced cataractous goat eye lens. In the in-vivo group, cataract was induced in rats by 30% galactose diet alone (control) or with the addition of Abutilon indicum (treated group). The results indicate the dose-dependent in-vitro antioxidant activity against DPPH, Reducing power assay, Phosphomolybdenum antioxidant assay, Nitric oxide-scavenging activity, Hydrogen peroxide scavenging, and Hydroxyl radical scavenging comparable with that of standard Ascorbic acid and also appreciable α-amylase inhibitory activity with IC50 values comparable with that of standard Acarbose. An in-vitro study was conducted, which reported that the lens group...
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Posted by admin on Jan 31, 2023 in |
The present investigation was carried out to analyze the bioactive metabolites of red seaweed Champia compressa collected from Mandapam, Ramanathapuram district, Tamil Nadu, India. The collected sample was evaluated for qualitative and quantitative phytochemical screening analysis using five different solvents methods. The preliminary qualitative analysis of Champia compressa solvent extracts revealed the presence of flavonoids, tannins, steroids, cardiac glycosides, terpenoids, coumarins, alkaloids, saponins, and phenols. Further quantitative phytochemical analysis was performed using standard procedures that revealed the presence of the highest phenol content (506.67 mg GAE/g) found in aqueous extract and the lowest in chloroform extract (8.33 mg GAE/g). Similarly, alkaloid content (34.09 mg AE/g) was highest in ethyl acetate extract and lowest (15 mg AE/g) in aqueous extract. The highest steroid content (85.88 mg BSE/g) was observed in hexane extract, whereas the lowest (3.69 mg BSE/g) in chloroform extract, however terpenoid (30.25 mg LE/g) was higher in methanolic extract, and the least (3.38 mg LE/g) found in aqueous extract respectively. The FTIR characterization represents the functional groups of...
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Posted by admin on Jan 31, 2023 in |
Citrus sinensis are treasuries to deliver novel drugs and a significant aromatic medicinal plant reported to possess a broad spectrum of medicinal uses. The compounds present in these plants can deliver the potential therapeutic drug. A free web tool, SWISS ADME predictor, was used to evaluate the pharmacokinetics, drug-likeness, and medicinal chemistry friendliness of phytoconstituents under investigation. The in-silico study has been deployed to screen the phytochemical components D-limonene, α-pinene, and β-pinene in Citrus sinensis. Against the protein target of RNA-dependent RNA-polymerase and Spike receptor binding domain with the aid of AutoDockVina software. All calculations for protein-fixed ligand-flexible docking were done using the Lamarckian Genetic Algorithm (LGA) method and analyzed using BIOVIA Discovery Studio 2016.13. The Phytoconstituents also showed good hydrophilic-lipophilic balance, good bioavailability, and decent GI absorption. The results obtained after docking showed a good binding affinity and implicated the active phytoconstituents for drug discovery and...
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