Posted by admin on Jun 30, 2023 in |
A novel, simple, accurate, precise, robust High-Performance Liquid Chromatography method was developed to simultaneously estimate reserpine and piperine. This method was applied to standardize polyherbal formulation containing these markers as one of its ingredients. The markers were resolved using Prontosil C-18 column, with mobile phase acetonitrile and water containing 0.05% ortho-phosphoric acid in the ratio 50:50, flow rate of 1 ml/min and detector set at 225 nm. Retention time of reserpine and piperine were found to be 5.4±0.2 min and 13.5 ± 0.2 min, respectively. Linear response was obtained in the tested concentration range of 1 to 20 µg/ml for reserpine and 10 to 100 µg/ml for piperine, with correlation coefficients of 0.999 and 0.998 for reserpine and piperine, respectively. This method can be used to evaluate other formulations containing reserpine and piperine as markers, thus conforming to the need of ensuring safety and quality of herbal...
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Posted by admin on Jun 30, 2023 in |
In this manuscript, a fast, stability-indicating RP-HPLC method with UV detection was developed and validated to quantify doripenem in the bulk drug and its injectable formulation. The analysis was performed using Phenomenex (250 x 4.6mm, 5µm) ODS column with a flow rate of 1 mL min–1 and a SPD 20 A UV detector to monitor the eluate at 316 nm. The mobile phase was acetonitrile and 10 mM ammonium formate (pH=3.5) in the ratio 35:65, in isocratic mode. The linearity range is 25–250 μg mL-1 and the linear regression analysis data for the calibration plot showed good linear relationship with R2 = 0.9999. The LOD and LOQ were 213 and 638 ng mL-1 respectively. The stress conditions employed include acid, alkali, water, hydrogen peroxide, dry heat and UV light. The drug peak was well resolved from the degradation products’ peaks; hence, the method can be used to analyze stability samples...
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Posted by admin on Jun 30, 2023 in |
A wound is an injury of living tissue or a break in the epithelial integrity of the upper layer of skin. This may lead to disturbance of skin anatomical structure and functions. Nowadays, wound healing is a challenging clinical problem. So, effective wound management is required. Different in-vitro, in-vivo and ex-vivo models have been developed to evaluate wound healing activity. The present study aims to evaluate the in-vitro wound healing potential of methanol extract of starfish Protoreaster linckii on the Human L-32 Cell line through scratch assay. The wound-healing properties of the Protoreaster linckii extract at different concentrations (25µg/ml, 50µg/ml and 100µg/ml) were assessed. The wound closure and migration of cells were directly related to the concentrations of the extract. The extract concentration increased from 25µg/ml to 100µg/ml and the percentage of wound healing (40% – 98%) also increased. The results showed that the methanol extract of P. linckii has potential wound-healing activity and it can serve as an alternative therapy against synthetic drugs for wound...
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Posted by admin on Jun 30, 2023 in |
The main aim of the current research is to formulate and evaluate the anti-aging cream containing lactic acid and cocoa butter. Skin health and beauty is regarded as one of the most important variables influencing general “well-being” and “health” perception in humans because of that, various anti-aging techniques have been created in recent years. Uneven pigmentation, increased wrinkles, loss of elasticity, dryness, and roughness are all signs of skin ageing. Lactic acid dissolves the connections that hold old, dull cells together on the skin’s surface, allowing them to be removed. Exfoliation is the term for this procedure. Lactic acid helps your skin shed old cells and replace them with new ones by speeding up cell turnover and stimulating cell renewal. As a result of exfoliation, lactic acid prevents and delay new fine lines and wrinkles from developing. Cocoa butter used in the formulation, gives the skin moisture and helps to remove the dark spots because of its anti-oxidant...
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Posted by admin on Jun 30, 2023 in |
Heavy metal pollutants in tannery effluent can be removed using the bioremediation technique. This study intends to extract bacteria that may reduce heavy metals and molecularly characterize them using 16S rRNA PCR in samples taken from tannery effluent. It also studies their cell-free immobilized enzymatic activity. Thirteen bacterial isolates were identified based on morphological, cultural, and biochemical characteristics. The top four isolates, A, B, sp5, and sp3, were identified as Brevundimonas sp, Proteus mirabilis, and Alcaligenes faecalis by morphological, cultural, biochemical, MALDI-TOF Analysis, and molecular characteristics using 16S rRNA. These organisms showed good tolerance to zinc, nickel, and chromium. When heavy metals like zinc and chromium have reduction properties, by ICP-OES to assess the concentration of heavy metals. Further investigation, beads were immobilized, and its activity was...
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