Posted by admin on Mar 31, 2023 in |
The present study evaluated the bioactive components, antioxidant and anti-diabetic potential in the methanolic extract of edible Brotia costula. The identification of the sample was confirmed with the help of the DNA barcoding technique. The COI gene sequence was submitted in NCBI and obtained the accession number OM056887. Bioactive compounds present in the sample were evaluated and identified with the help of the Gas chromatography-mass spectroscopy (GC-MS) technique. The methanolic extract was tested for antioxidant capacity by its scavenging activity against Diphenylpicrylhydrazyl (DPPH), and its anti-diabetic activity was measured by the methanolic extract Alpha- amylase inhibition method. The GC-MS study revealed the presence of 76 bioactive components, and the compound with the highest peak area is phenol, 2, 4-bis (1, 1-dimethylethyl)-, phosphite. An inhibition percentage of 69.89 % in 500 μg against DPPH of the sample was observed as compared to 85.77% in ascorbic acid. The sample showed a good anti-diabetic potential with an inhibition percentage of 87.80 % in 500 μg of the sample. The findings of this...
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Posted by admin on Mar 31, 2023 in |
Aim: The current research aimed to formulate and evaluate herbal nanoemulsion of poorly soluble Berberine hydrochloride, which can provide a realistic alternative for treating microbial infections. Materials and Methods: The drug was characterized using various analytical techniques such as Ultraviolet Spectroscopy, Fourier transform infrared spectroscopy, and Differential Scanning Calorimetry study. The herbal nanoemulsion was prepared using the water titration method by constructing pseudo-ternary phase diagrams. Formulations were evaluated by thermodynamic stability studies, droplet size analysis, viscosity, drug content, refractive index, polydispersity, and in-vitro release study. Results: On the basis of the analytical study, no interaction between the drug and excipients was found. Based on visual observation nanoemulsion was optimized by using thermodynamic stability study and freeze-thaw cycle. The optimized nanoemulsion (NE-2) shows droplet size of 203.7 nm, Viscosity-94 centipoises, Zeta potential -19mv, polydispersity index- 0.366. The optimized formulation NE-2 shows 91.7% drug release in 24 hours and was further evaluated for antimicrobial activity using Bacillus subtilis and Candida albicans. The zone of inhibition was found to be 56.26± 1.78 mm for...
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Posted by admin on Mar 31, 2023 in |
This work compared the effect of hot aqueous extraction and blending on phytochemical content, AgNPs synthesis, antibacterial and antioxidant properties of ginger and turmeric. Qualitative and quantitative phytochemical screening was conducted on a hot aqueous extract of ginger and turmeric combination (CHAE) and blended ginger & turmeric combination (CBLE). An attempt was then made to synthesize silver nanoparticles by reducing 1mM AgNO3 solution with CHAE & CBLE, respectively. The biosynthesized AgNPs were characterized using UV-Visible spectroscopy, FTIR, and SEM. Using existing protocols, the antioxidant and antibacterial properties of CHAE, CBLE, and biosynthesized AgNPs were evaluated. The result of the phytochemical screening revealed the presence of phytochemicals required for synthesizing silver nanoparticles, such as flavonoids and phenols in CBLE but absented in CHAE. The UV-Visible spectrum confirmed the synthesis of AgNPs using CBLE (AgNPs-CBLE), with the highest absorbance peak observed at 412 nm. The FTIR analysis revealed the involvement of phenolic compounds in the bioreduction of Ag+ as well as the capping/stabilization of the biosynthesized nanoparticles. AgNPs-CBLE were irregularly granulated and...
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Posted by admin on Mar 31, 2023 in |
Itraconazole is a triazole antifungal category drug used for the treatment of local &Systemic fungal infections. Itraconazole is not recommended to neutropenic and other immune-compromised patients who have difficulty swallowing the oral capsule formulation, and it has many side effects. Commercially, Itraconazole topical gel preparation is not available in the market; thus, this formulation is made for better patient compliance, reduces the drug dose, and avoids side effects like liver damage and kidney damage avoiding first-pass metabolism. The gel was formulated by preparing nanocomposite with polymer inclusion complex by microwave-induced diffusion technique. Which ultimately results in enhancing the water solubility of nanocomposites. The resultant polymer-based nanocomposite retains the inherent properties nanoparticles, while providing higher stability, and this nanocomposite is incorporated into the gel base. Prepared nanogel Prepared nanogel is characterized by pH determination, Spreadability, Viscosity measurement, In-vitro diffusion, antifungal activity. Among the formulation, F3 was selected as the best formulation having % CDR of 76.57 % & more Antifungal activity than the marketed formulation. The results indicated that nanocomposite-based...
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Posted by admin on Mar 31, 2023 in |
Objectives: Cyclophosphamide is an alkylating agent known for its reproductive toxicity in males. This study evaluates the protective effects of Withania somnifera extract (WSE) on cyclophosphamide (CP) induced testicular toxicity and DNA damage in male rats. Methods: Adult male rats were randomly divided into four groups Group1-Control, Group 2- Cyclophosphamide, Group 3- Withania somnifera extract, Group4- Cyclophosphamide+ Withania somnifera. After the experimental period of 8 weeks, sperm quality, hormonal analysis, and DNA damage studies were carried out. Histopathological analysis of testes was performed for the assessment of spermatogenic disorders. Results: In CP-treated rats, sperm quality was reduced with decreased testosterone levels with increased DNA damage. Co-administration of WSE with CP improved sperm quality, testosterone levels, spermatogenesis and genotoxicity of cyclophosphamide. The histopathological study also restored most of the degenerative and necrotic changes. Conclusions: This study suggests a prominent role of WS against CP-induced testicular toxicity and DNA...
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