Posted by admin on Jan 31, 2023 in |
A novel, rapid, isocratic, reversed-phase HPLC method was developed to simultaneously determine betamethasone dipropionate, miconazole nitrate and chlorocresol in a topical dosage form. The separation was performed on the C18 prontosil (250 x 4.6mm x 5µ) column. The mobile phase used for this study consists of acetonitrile and phosphate buffer of pH 2 in the ratio of 70:30 v/v, respectively. The flow rate was set as 1 ml per minute. This method used a UV detector with a wavelength of 235 nm. The retention time of chlorocresol, miconazole nitrate, and betamethasone dipropionate was 4.30 min, 5.13min, and 6.49 min, respectively. Linearity was observed for betamethasone dipropionate, miconazole nitrate, and chlorocresol in the concentration range of 1 to 3 µg/ml, 40 to 120 µg/ml and 2 to 6 µg/ml, respectively. The novel, rapid, reversed-phase HPLC method can be used in the regular analysis of any drug or combination in the pharmaceutical dosage...
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Posted by admin on Jan 31, 2023 in |
The present study estimated the antidiabetic activity of methanolic extract of plant fruits of plant Withania coagulans L. Dunal. Withania coagulans (family Solanaceae) is commonly known as “Paneer ke phool” in Hindi and “Indian cheese maker” or “Vegetable rennet” in English, distributed in drier parts of India. The primary aim of this study was to confirm antidiabetic action of Withania coagulans by using the in-vitro method of glucose uptake by yeast cells using methanolic fruit extract of the sample. It is reported that the Withanolides are responsible for its anti-hyperglycemic activity, which has been standardized by the TLC method using 50% Sulphuric acid and dragendroff’s reagents as a spraying agent in this study. The current research found that Withania coagulans methanolic fruit extract improved glucose absorption in yeast cells by 0–90% at various glucose concentrations (5, 10, and 25 mM). Metformin was used as a standard reference. At all glucose concentrations, the highest concentration of WCME (5 mg/ml WCME) demonstrated maximum activity and showed the greatest increase (81.83 percent)...
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Posted by admin on Jan 31, 2023 in |
The oyster mushroom (Pleurotus ostreatus) is a culinary mushroom. Mushrooms are considered a potential source of many essential nutrients and therapeutic bioactive compounds. Pleurotus ostreatus is a macro fungus that belongs to the class Basidiomycetes and the family Pleurotaceae, known as the oyster mushroom. It is the most important commercially cultivated mushroom in the world. Mushroom contains rich nutrients such as proteins, carbohydrates, vitamins, amino acids, lipids and fibers. It is used as food and medicine. Mushroom contains more bioactive compounds such as peptides, polysaccharides, liposaccharides, glycoprotein, lectin, triterpenoid, fatty acids, essential amino acids and nucleosides. They are used traditionally as a medicine for different diseases. These mushrooms have been reported to be antioxidant, anticancer, antimicrobial, anti- inflammatory, antidiabetic, antihypertensive, antihypercholesterolemic, antiatherogenic, anti-hyperglycemic and immunomodulating. This review gives information on the traditional uses, chemical composition and nutritional benefits of oyster mushrooms. These studies reveal that Pleurotus ostreatus is a source of medicinally active compounds with various pharmacological effects. These studies will be helpful to create interest in Pleurotus ostreatus...
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Posted by admin on Jan 31, 2023 in |
Background: The present study aimed to evaluate the hepatoprotective activity of aqueous extract of Acalypha communis in Wistar rats. Methods: The hepatoprotective activity was studied by Thioacetamide (TAA) (100 mg/kg b.wt.i.p) and Rifampicin (RMP) (100 mg/kg, po) induced models. Acute toxicity studies and preliminary phytochemical screening were also studied to evaluate the toxicity. Results: No toxicity profile was observed in rats after oral administration of the ethanolic leaf extract at 5mg/kg body weight. The different doses of 200 mg/kg and 400 mg/kg were administered with the extract of Acalypha communis; there was significant (P < 0.001) reduction in Biochemical parameters with respect to control. Phytochemical screening of the plant extract revealed the presence of tannins, alkaloids, flavonoids, saponins, and terpenoids. Conclusion: It can be concluded that the hepatoprotective activity elucidated by Acalypha communis could be mainly due to the presence of a high-value class of compound like the phenolic group as the major content in the...
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Posted by admin on Jan 31, 2023 in |
Few studies are available to study the drug utilization pattern and adverse drug reactions of anti-epileptic drugs (AEDs) in paediatric population. Hence, this study was planned with the primary objective of studying the drug utilization pattern of anti-epileptic drugs in a tertiary care centre pediatric population. The secondary objective was to assess adverse drug reactions in the same population. Data from 214 pediatric patients visiting Neurology OPD was reviewed in this prospective, cross-sectional, non-interventional, observational study for 6 months. Patients aged 1 to 12 years, of either sex, diagnosed with epilepsy, and prescribed at least one anti-epileptic drug were included in the study. Sodium valproate was the most frequently prescribed anti-epileptic drug, followed by levetiracetam, carbamazepine, phenytoin, and clobazam. 49.07% of patients received monotherapy while 50.93% of patients received polytherapy. Out of the 39 adverse drug reactions (ADR) reported, the most common ADR was drowsiness (23.08%), followed by weight gain (17.95%), lethargy (15.38%), rash (10.26%), irritability (7.69%), decreased concentration, headache, vomiting (5.13%). The most common drug prescribed for generalized...
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