Posted by admin on Sep 30, 2022 in |
The use of natural compounds has become more preferable for reducing the severe complications of diabetes mellitus due to having a minimum cost and the least side effects. The recent study is aimed to diagnose in-vitro α-amylase inhibition activity and the molecular interactions of various phytocompounds like momordicoside A, momordicoside B, momordicoside C, momordicoside D, momor-dicoside E, gallic acid, β-sitosterol, chlorogenic acid, vicine, gentisic acid, epicatechin, apiole, campesterol, cycloeucalenol, lophenol, cisdihydrocarveol, transnerolidol, daucosterol, arachidic acid, lauric acid, palmitic acid, momordenol, obtusifoliol, γ-linoleic acid, steric acid, oleic acid, capric acid and myristic acid present in seeds of Momordica charantia Linn. The molecular docking of the 3D structure of these compounds has been studied with target diabetic protein i.e., α-amylase. 2D interactions were also studied by using Ligand Interaction Diagram. The interaction fingerprinting was generated using the Interaction fingerprint tool available in Maestro (version 12.5) of Schrodinger Suite version 2020-3. The results demonstrated that bioactive compounds i.e. momordicoside A, momordicoside C, momordicoside D and momordicoside B, with a higher binding affinity...
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Posted by admin on Sep 30, 2022 in |
Sensitive and selective liquid chromatography/tandem mass spectrometry (LC–MS/MS) method was developed and validated for warfarin (WAR) in buffered SD (Sprague Dawley) rat plasma (PBS and Plasma: 50:50, v/v) using propranolol (PRP) as internal standards (IS) with the Plasma protein binding (PPB) assay samples to a determination of free and total warfarin. Plasma protein precipitation extraction technique with acetonitrile containing internal standard was used for extraction of the analyte. A generic gradient method with a short run time of 2.0 min was developed for the analysis of warfarin with a C18 analytical column (Synrgi 4 µm Fusion-RP, 50X2 mm), mobile phase composed of aqueous phase: 0.1% formic acid containing Milli-Q-water and Organic Phase 0.1% formic acid containing acetonitrile. Detection was performed on a triple quadrupole mass spectrometer that employed the Positive electrospray ionization (ESI) technique, operating in multiple reaction monitoring (MRM), with the transitions of m/z 309.2-163.1. The analysis was carried out over a linear concentration range of 1–1200 nM, where the 0.1% of the free fraction of the compound...
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Posted by admin on Sep 30, 2022 in |
Background: Halela (Terminalia chebula Retz) is one of the constituents in the well-known Unani compound medicine Itriphal, which has been used for thousands of years to treat a variety of ailments. In order to improve the therapeutic activity of the drug, the present study adapted the process of Tadbeer / Mudabbar (rectification/purification) which is based on the concept of Islah-e-Advia. This process thus helps in making the drug effective, safe, and specific, or minimizing undesirable characteristics by acting on the drug’s constituents without compromising its medicinal value through various methods (rectification process). Objectives: To further grasp the significance of the notion of Islah-e-Advia, before and after Halela Mudabbar (rectification), a comparative analysis was conducted. Methods: Mudabbar was done by roasting dry Halela powder with Almond oil for 7-10 minutes on a low flame on standard cooking gas. Physico-chemical and quantitative HPLC examinations of gallic acid, ellagic acid, chebulagic acid, and chebulinic acid were performed with Halela before and after the Mudabbar procedure. Results: Quantitative HPLC analysis of gallic acid,...
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Posted by admin on Sep 30, 2022 in |
Anorexia nervosa (AN) is a life-threatening condition with the highest mortality rate of all psychiatric disorders. The core physiological features of ANare the extreme overvaluation of shape and weight. Severe AN causes cardiovascular complications and majorly refeeding syndrome. The prominent features of refeeding syndrome include severe hypo-phosphatemia, hypokalemia and hypomagnesia, and other fluid imbalance abnormalities, vitamin deficiencies, volume overload, and edema. Most cases are brought to clinical attention only when there are severe somatic complaints. According to the Global Leadership Initiative on Malnutrition (GLIM) classification, the patients with AN present chronic disease-related malnutrition without inflammation with different degrees of severity. To be diagnosed with anorexia nervosa a person must meet all of the current DSM 5 criteria. We cannot pinpoint a single cause for anorexia nervosa. It is caused by the complex interaction of various genetic and psychosocial factors, other risk factors include: stress, social isolation, poor emotion regulation strategies, and general psychiatric co-morbidities. In anorexia, Nutritional supplements can improve weight gain, which can reverse the abnormal hematological factors,...
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Posted by admin on Sep 30, 2022 in |
The present work deals with the formulation of containing aqueous extract of Tectona grandis (Teak) Leaves and prepared formulation against some Gram-positive and Gram-negative bacteria. The antibacterial activity of various T. grandis leaves extracts was measured by determining minimum inhibitory concentration (MIC) by cube dilution method and zone of inhibition by disc diffusion method against P. aeruginosa, Aqueous T. grandis Leaves extracts exhibited comparatively higher antibacterial potential in comparison with other extracts. All the prepared herbal formulations were subjected to antibacterial study by determining the zone of inhibition by disc diffusion. Herbal formulations containing gel aqueous exhibited better zones of inhibition against P. aeruginosa in comparison with others. This type of bacteria (germ) is found commonly in the environment, like in soil and water. From the results of this study, it can be concluded that herbal formulations containing gel aqueous T. grandis leave extract are found suitable for use against bacterial infections in the form of topical gels or another suitable dosage...
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