Posted by admin on Sep 30, 2022 in |
Background and Objectives: As per requisition of current regulatory requirements, the simple, rapid and sensitive method by 33 factorial QbD approach was established and validated for Bilastine (BST) and Montelukast (MNT) by RP-HPLC. Method: A simple RP-HPLC method has been developed and validated with different parameters such as linearity, precision, repeatability, LOD, LOQ, accuracy as per International Conference for Harmonisation guidelines (Q2R1). Statistical data analysis was done for data obtained from different aliquots Runs on Agilent Tech. Gradient System with Autoinjector, UV (DAD) & Gradient Detector. Results: Equipped with Reverse Phase (Agilent) C18 column (4.6mm x 100mm; 5µm), a 20µl injection loop and UV730D Absorbance detector at 226nm wavelength and running chemstation 10.1 software and drugs along with degradants were separated via acetonitrile: water 0.1% OPA (45:55), of pH 3 as mobile phase setting flow rate 0.8 ml/min at ambient temperature. The developed method was found linear over the concentration range of 10-50 /ml for BST and 5-25 μg/ml for MNT, while detection and quantitation limit were found to...
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Posted by admin on Sep 30, 2022 in |
Concurrent administration of herbal medicines and conventional drugs is a common practice globally, especially as the patronage of medicinal plants increases across the world. This study aimed to determine the effect of Cryptolepis sanguinolenta administration on the metabolism of chloroquine by evaluating specific Cytochrome P450 isozymes. Reconstituted freeze dried Cryptolepis sanguinolenta was administered to male Sprague-Dawley rats in drinking water for 2 weeks at a dose of 36mg/kg, followed by a single dose of chloroquine (15mg/kg). All the animals were sacrificed by cervical dislocation after 24 h of final drug administration, and the liver was excised for hepatic microsomes preparation. Using the microsomes, the PNPH, AmD and NOD activities were determined as a measure of CYP2E1, CYP2B1 and CYP1A activities, respectively. The study showed that concurrent administration of chloroquine and Cryptolepis sanguinolenta impaired the CYP-mediated metabolism of chloroquine due to competitive inhibition of CYP2B1 by C. sanguinolenta. However, concurrent administration of chloroquine and Cryptolepissanguinolenta showed no significant effect on CYP2E1 and CYP1A. Patients should be advised on the clinical...
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Posted by admin on Sep 30, 2022 in |
Introduction: Covid-19 pandemic has posed new challenges to humanity. The definitive cure is yet to be found, while drug repurposing holds the key so far. Remdesivir had been used for the treatment of novel corona-virus. Ivermectin and Doxycycline have also been recommended for treating Covid-19, but their efficacy has not been studied extensively. Methods: We conducted a retrospective study during the second wave of the pandemic. Data were retrieved and 162 patients who had received Remdesivir were divided into 2 groups. Group I (101 patients) received Tab Ivermectin and Cap Doxycycline and Group R (61 patients) received only Remdesivir. The standard treatment was common for both groups. Mortality, duration of intensive care unit stay, and oxygen requirement were compared between the groups. Results: There was no statistically significant difference observed in demography, vaccination status, investigations on admission, comorbidity, and APACHE scoring between both groups. Group R had higher mortality with 75.41% as compared to Group I with a mortality rate of 55.45%, which was statistically significant. The mean duration...
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Posted by admin on Sep 30, 2022 in |
Background: Stress is an important factor determining the mental health of the individual. During this pandemic, a student’s life has had a questionable influence in academics since an online platform was created to educate them. This study was done to assess the stress level among medical and engineering students. Materials and Methods: A cross-sectional study was conducted to assess the stress level. A 15 close-ended questionnaire was validated before circulating among the students through Google forms to various colleges. Statistical analysis was done using SPSS software version 26.0, Descriptive statistics was done to assess the mean and standard deviation and a chi-square test was done to find an association among the students. The P-value <0.05 was considered to be statistically significant. Results: The study was done among the 182 students to assess their stress level. The study results showed that the stress level was found to be almost same among the medical and engineering students, whereas while assessing the online platform to conduct academic classes, it was shown that...
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Posted by admin on Sep 30, 2022 in |
The present study aimed to study the effect of the drug Xymedon and its conjugate with L-ascorbic acid exhibiting hepatoprotective activity on the apoptosis of rat liver cells against the background of the influence of hepatotoxic agent tetrachloromethane. Though the general effects of Xymedon and its conjugates with various biogenic acids have been studied, the molecular markers affected by the compounds have not yet been established. Experimental methods of investigation included modeling of toxic liver damage by oral administration of carbon tetrachloride to laboratory Wistar rats. To establish the molecular mechanisms of the antiapoptotic effect involved in the hepatoprotective activity of Xymedon and its conjugate with L-ascorbic acid, the level of early apoptosis markers Akt, BAD, BCL-2, p53, Active Caspase-8 Active Caspase-9 in rat liver homogenates was determined by multiplex analysis method using the MagPix system («MerkMillipore», USA). The study showed that Xymedon and its derivative with L-ascorbic acid exhibited an antiapoptotic effect, significantly reducing the number of early apoptosis markers BAD, Active Caspase-9. Also, the derivative with L-ascorbic...
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